Anti-GDNF 抗体 [EPR2714(N)] (ab176564)
![Western blot - Anti-GDNF antibody [EPR2714(N)] (ab176564)](https://www.abcam.co.jp/ps/products/176/ab176564/Images/ab176564-204269-anti-gdnf-antibody-epr2714n-western-blot.jpg "Western blot - Anti-GDNF antibody [EPR2714(N)] (ab176564)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2714(N)] to GDNF
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GDNF antibody [EPR2714(N)]
GDNF 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2714(N)] to GDNF -
由来種
Rabbit -
特異性
GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot. -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human GDNF aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P39905 -
ポジティブ･コントロール
- SH-SY5Y and HepG2 whole cell lysate (ab7900), SH-SY5Y cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2714(N) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab176564の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot. |
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 24 kDa. GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot. |
ターゲット情報
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機能
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. -
組織特異性
In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. -
関連疾患
Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. -
配列類似性
Belongs to the TGF-beta family. GDNF subfamily. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 2668 Human
- Entrez Gene: 14573 Mouse
- Entrez Gene: 25453 Rat
- Omim: 600837 Human
- SwissProt: P39905 Human
- SwissProt: P48540 Mouse
- SwissProt: Q07731 Rat
- Unigene: 248114 Human
see all -
別名
- Astrocyte derived trophic factor antibody
- Astrocyte derived trophic factor 1 antibody
- Astrocyte-derived trophic factor antibody
see all
画像
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Western blot - Anti-GDNF antibody [EPR2714(N)] (ab176564)All lanes : Anti-GDNF antibody [EPR2714(N)] (ab176564) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 24 kDa -
![Flow Cytometry (Intracellular) - Anti-GDNF antibody [EPR2714(N)] (ab176564)](https://www.abcam.co.jp/ps/products/176/ab176564/Images/ab176564-204268-anti-gdnf-antibody-epr2714n-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-GDNF antibody [EPR2714(N)] (ab176564)Intracellular Flow Cytometry analysis of permeabilized SH-SY5Y cells labeling GDNF (red) with ab176564 at a 1/10 dilution, or negative control rabbit IgG (green)
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![Anti-GDNF antibody [EPR2714(N)] (ab176564)](https://www.abcam.co.jp/ps/products/176/ab176564/Images/ab176564-3-benefits-of-recombinant-antibodies.png)
Anti-GDNF antibody [EPR2714(N)] (ab176564)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab176564 は 17 報の論文で使用されています。
- Ke N et al. Downregulation of miR-211-5p Promotes Carboplatin Resistance in Human Retinoblastoma Y79 Cells by Affecting the GDNF-LIF Interaction. Front Oncol 12:848733 (2022). PubMed: 35311096
- Zhang S et al. Nerve regeneration in rat peripheral nerve allografts: An assessment of the role of endogenous neurotrophic factors in nerve cryopreservation and regeneration. Eur J Neurosci 55:1895-1916 (2022). PubMed: 35332602
- Lv W et al. miR-22-5p regulates the self-renewal of spermatogonial stem cells by targeting EZH2. Open Med (Wars) 17:556-565 (2022). PubMed: 35415251
- Lin C et al. S1P promotes corneal trigeminal neuron differentiation and corneal nerve repair via upregulating nerve growth factor expression in a mouse model. Open Life Sci 17:1324-1332 (2022). PubMed: 36313859
- Arato I et al. Effect of EPA on Neonatal Pig Sertoli Cells "In Vitro": A Possible Treatment to Help Maintain Fertility in Pre-Pubertal Boys Undergoing Treatment With Gonado-Toxic Therapies. Front Endocrinol (Lausanne) 12:694796 (2021). PubMed: 34093450