Anti-GDI1 抗体 [EPR13195] (ab181213)
![Western blot - Anti-GDI1 antibody [EPR13195] (ab181213)](https://www.abcam.co.jp/ps/products/181/ab181213/Images/ab181213-215107-anti-gdi1-antibody-epr13195-western-blot.png "Western blot - Anti-GDI1 antibody [EPR13195] (ab181213)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13195] to GDI1
- Suitable for: Flow Cyt (Intra), IP, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GDI1 antibody [EPR13195]
GDI1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13195] to GDI1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- U87 MG, HeLa, SH-SY5Y and Human fetal brain lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR13195 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181213の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/40.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP |
1/30 - 1/50.
|
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| WB |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IP
1/30 - 1/50. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa). |
ターゲット情報
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機能
Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. -
組織特異性
Brain; predominant in neural and sensory tissues. -
関連疾患
Defects in GDI1 are the cause of mental retardation X-linked type 41 (MRX41) [MIM:300104]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
Defects in GDI1 are the cause of mental retardation X-linked type 48 (MRX48) [MIM:300104]; also known as MRX3. -
配列類似性
Belongs to the Rab GDI family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 2664 Human
- Entrez Gene: 14567 Mouse
- Entrez Gene: 25183 Rat
- Omim: 300104 Human
- SwissProt: P31150 Human
- SwissProt: P50396 Mouse
- SwissProt: P50398 Rat
- Unigene: 74576 Human
see all -
別名
- 1A antibody
- FLJ41411 antibody
- GDI-1 antibody
see all
画像
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Western blot - Anti-GDI1 antibody [EPR13195] (ab181213)All lanes : Anti-GDI1 antibody [EPR13195] (ab181213) at 1/10000 dilution
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : Human fetal brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa -
![Flow Cytometry (Intracellular) - Anti-GDI1 antibody [EPR13195] (ab181213)](https://www.abcam.co.jp/ps/products/181/ab181213/Images/ab181213-215108-anti-gdi1-antibody-epr13195-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-GDI1 antibody [EPR13195] (ab181213)Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling GDI1 with ab181213at 1/40 dilution (red), or a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as a secondary antibody.
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![Immunoprecipitation - Anti-GDI1 antibody [EPR13195] (ab181213)](https://www.abcam.co.jp/ps/products/181/ab181213/Images/ab181213-215109-anti-gdi1-antibody-epr13195-immunoprecipitation.jpg)
Immunoprecipitation - Anti-GDI1 antibody [EPR13195] (ab181213)Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181213 at 1/50 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Anti-GDI1 antibody [EPR13195] (ab181213)](https://www.abcam.co.jp/ps/products/181/ab181213/Images/ab181213-4-benefits-of-recombinant-antibodies.png)
Anti-GDI1 antibody [EPR13195] (ab181213)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab181213 は論文での使用が確認できていません。