Anti-GDF15 抗体 (ab39999)
Key features and details
- Goat polyclonal to GDF15
- Suitable for: WB, IHC-Fr, ICC/IF, IHC-P, Flow Cyt (Intra)
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-GDF15 antibody
GDF15 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to GDF15 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Fr, ICC/IF, IHC-P, Flow Cyt (Intra)more details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse
-
免疫原
Synthetic peptide: QKTDTGVSLQTYDD, corresponding to C terminal amino acids 286-299 of Human GDF15
-
ポジティブ･コントロール
- Flow Cyt (Intra): HeLa cells. WB: Human prostate lysates, A549 cell lysate, CaCo-2 cell lysate, LNCaP cell lysate.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab39999の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 0.1 µg/ml. Detects a band of approximately 30-35 kDa (predicted molecular weight: 34 kDa).
1 hour primary incubation is recommended for this product. |
| IHC-Fr | (1) |
Use at an assay dependent concentration.
See Abreview. |
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| IHC-P | (1) |
Use a concentration of 7 µg/ml.
|
| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 µg/ml. Detects a band of approximately 30-35 kDa (predicted molecular weight: 34 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-Fr
Use at an assay dependent concentration. See Abreview. |
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-P
Use a concentration of 7 µg/ml. |
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
ターゲット情報
-
組織特異性
Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. -
配列類似性
Belongs to the TGF-beta family. -
細胞内局在
Secreted. - Information by UniProt
-
参照データベース
- Entrez Gene: 9518 Human
- Entrez Gene: 23886 Mouse
- Entrez Gene: 29455 Rat
- Omim: 605312 Human
- SwissProt: Q99988 Human
- SwissProt: Q9Z0J7 Mouse
- SwissProt: Q9Z0J6 Rat
- Unigene: 616962 Human
see all -
別名
- GDF 15 antibody
- GDF-15 antibody
- Gdf15 antibody
see all
画像
-
Western blot - Anti-GDF15 antibody (ab39999)Lanes 1-2 : Anti-GDF15 antibody (ab39999) at 0.1 µg/ml
Lane 3 : Anti-GDF15 antibody (ab39999) at 0.3 µg/ml
Lane 1 : A549 cell lysate
Lane 2 : CaCo-2 cell lysate
Lane 3 : LNCaP cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 34 kDaDetected by chemiluminescence.
-
Western blot - Anti-GDF15 antibody (ab39999)Anti-GDF15 antibody (ab39999) at 0.5 µg/ml + Human prostate whole cell lysate (protein in RIPA buffer) at 35 µg
Predicted band size: 34 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF15 antibody (ab39999)Immunhistochemical analysis of paraffin embedded Human placenta stained with ab39999 at 7 µg/ml. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
-
Immunocytochemistry/ Immunofluorescence - Anti-GDF15 antibody (ab39999)This image is courtesy of an abreview submitted by Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.ICC/IF image of human bone marrow smear stained with Ab39999(1/250). The smear was permeabilized in 0.1% PBS-Triton X for 1h and incubated with 10% donkey serum for 1hr at 24°C to block non-specific protein-protein interactions. The sections were then incubated with ab39999 used at 1/250 dilution, overnight at +4°C. The secondary antibody was Alexa Fluor® 568 donkey anti-goat used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Flow Cytometry (Intracellular) - Anti-GDF15 antibody (ab39999)Flow cytometric analysis of paraformaldehyde fixed, 0.5% Triton permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GDF-15 using ab39999 at 10 μg/ml followed by Alexa Fluor 488 secondary antibody (blue) at 1 μg/ml. Unimmunized goat IgG was used as isotype control, followed by Alexa Fluor 488 secondary antibody (black).
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (9)
ab39999 は 9 報の論文で使用されています。
- Mishra R et al. Transplanted allogeneic cardiac progenitor cells secrete GDF-15 and stimulate an active immune remodeling process in the ischemic myocardium. J Transl Med 20:323 (2022). PubMed: 35864544
- Lin XF et al. Therapeutic effect of psoralen on muscle atrophy induced by tumor necrosis factor-a. Iran J Basic Med Sci 23:251-256 (2020). PubMed: 32405369
- Malik VA et al. GDF15 promotes simultaneous astrocyte remodeling and tight junction strengthening at the blood-brain barrier. J Neurosci Res 98:1433-1456 (2020). PubMed: 32170776
- Xiang Y et al. In vitro expansion affects the response of human bone marrow stromal cells to irradiation. Stem Cell Res Ther 10:82 (2019). PubMed: 30850008
- Gabriel KN et al. Association and regulation of protein factors of field effect in prostate tissues. Int J Oncol 49:1541-1552 (2016). ICC/IF ; Human . PubMed: 27634112
- Zhou YM et al. Growth differentiation factor-15 (GDF-15), novel biomarker for assessing atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease. Int J Clin Exp Med 8:21201-7 (2015). PubMed: 26885055
- Jones AC et al. Prostate field cancerization: deregulated expression of macrophage inhibitory cytokine 1 (MIC-1) and platelet derived growth factor A (PDGF-A) in tumor adjacent tissue. PLoS One 10:e0119314 (2015). IHC-P ; Human . PubMed: 25767870
- Hegde NV et al. Interrelationships between tissue iron status and erythropoiesis during postweaning development following neonatal iron deficiency in rats. Am J Physiol Gastrointest Liver Physiol 300:G470-6 (2011). WB ; Rat . PubMed: 21193529
- Chenau J et al. The cell line secretome, a suitable tool for investigating proteins released in vivo by tumors: application to the study of p53-modulated proteins secreted in lung cancer cells. J Proteome Res 8:4579-91 (2009). WB ; Human . PubMed: 19639960