Anti-GCNT3 抗体 (ab77728)
Key features and details
- Goat polyclonal to GCNT3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GCNT3 antibody
GCNT3 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to GCNT3 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey
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免疫原
Synthetic peptide:
C-NKFDPKVDDNALQ
, corresponding to internal sequence amino acids 410-422 of Human GCNT3 (NP_004742.1). -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab77728 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77728の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
| 特記事項 |
|---|
追加情報
Peptide ELISA: Antibody detection limit dilution 1/64000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 51 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 51 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development. -
組織特異性
Primarily expressed in mucus-secreting tissues. Expressed in colon, kidney, small intestine, trachea, and stomach, where mucin is produced. -
パスウェイ
Protein modification; protein glycosylation. -
配列類似性
Belongs to the glycosyltransferase 14 family. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Golgi apparatus membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 487578 Dog
- Entrez Gene: 100068109 Horse
- Entrez Gene: 9245 Human
- Entrez Gene: 72077 Mouse
- Entrez Gene: 286976 Rat
- Entrez Gene: 495681 Xenopus laevis
- Omim: 606836 Human
- SwissProt: O95395 Human
see all -
別名
- 2010013H22Rik antibody
- 2210021I22Rik antibody
- 2210401J11Rik antibody
see all
画像
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Western blot - Anti-GCNT3 antibody (ab77728)Anti-GCNT3 antibody (ab77728) at 0.1 µg/ml + Human Duodenum lysate (in RIPA buffer) at 35 µg
Predicted band size: 51 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab77728 は 2 報の論文で使用されています。
- Zhang Y et al. LINC00511 enhances LUAD malignancy by upregulating GCNT3 via miR-195-5p. BMC Cancer 22:389 (2022). PubMed: 35399076
- Li RW et al. The vitamin D receptor and inducible nitric oxide synthase associated pathways in acquired resistance to Cooperia oncophora infection in cattle. Vet Res 42:48 (2011). WB ; Cow . PubMed: 21414188