Anti-GCNA1 抗体 [TRA98] (ab82527)

Thank you for your inquiry and your patience with this reply.
For ab82527, it should be IgG2a, although we do not do any validation to verify that. The clone TRA98 recognizes a mouse testicular germ cell-specific antigen (1). In adult tissues, bands of 60-100 kDa proteins are detected only in the testis by Western blotting analysis with TRA98 (Fig on datasheet). The signals are observed in male and female embryos after embryonic day 12.5. The signal in male is detected during development of germ cells and also after birth, but the signal in female disappears by 5 days after birth. The antigen is localized only in the nuclear fraction of testicular germ cells and this antibody can be used for immunohistochemical staining of testicular germ cells (1, 2). The hybridoma producing TRA98 was obtained by immunization of a rat with the cell lysate of adult mouse testis by the group of Prof. Yoshitake Nishimune of Osaka University (1). This product was purified from serum-free culture medium of the hybridoma clone TRA98 by proprietary chromatography processes under mild conditions.
References: This product has been used in the following publications.
1.Tanaka H et al ” A germ cell-specific nuclear antigen recognized by a monoclonal antibody raised against mouse testicular germ cells” Int J Androl 20: 361-366 (1997) PMID: 9568529
2. Ohta H et al “Regulation of proliferation and differentiation in spermatogonial stem cells: the role of c-kit and its ligand SCF” Development 127: 2125-2131 (2000) PMID: 10769236
I think the other antibody you were looking at may have been clone TRA54 (IgM), ab92286. It is a different clone from TRA54 in that they recognize different proteins, although both target proteins are specific for haploid sperm cell-specific proteins. Dr. Tanaka who provided these clones, says the genes that code for the target proteins will be shortly published.
https://www.abcam.com/haploid-sperm-cell-specific-antigen-antibody-tra54-ab92286.html
I hope this information helps. Please contact us with any other questions.

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Thank you very much for your email.
I am happy to arrange a credit note or refund. However I would need more information about the problem customer experienced. Our customers feedback and own tested data show that the antibody detects Germ cells very nice so I would be interested in to know in which type of experiment the antibody wasn't working.
Many thanks for your cooperation in this case. I will look forward to hearing from you soon.

Thank you very much for your valuable feedback.

We would be very grateful if you could submit an Abreview about this product via the online product datasheet. We always encourage customers to send their results back to us.

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Thank you for contacting us. To answer your questions: 1) Yes, JLP is the same as SPAG9. For more alternative names, please check the protein database entry for the mouse protein: http://www.uniprot.org/uniprot/Q58A65 2)References are listed in the reference tab at the top of the datasheet. I added them here as well: i) Bagheri-Fam S et al. Defective survival of proliferating Sertoli cells and androgen receptor function in a mouse model of the ATR-X syndrome. Hum Mol Genet 20:2213-24 (2011). PubMed: 21427128 ii) Ohta H et al. Regulation of proliferation and differentiation in spermatogonial stem cells: the role of c-kit and its ligand SCF. Development 127:2125-31 (2000). IHC-Fr; Mouse. PubMed: 10769236 iii) Tanaka H et al. A germ cell-specific nuclear antigen recognized by a monoclonal antibody raised against mouse testicular germ cells. Int J Androl 20:361-6 (1997). WB; Mouse. PubMed: 9568529 3) The WB image was obtained fromthe supplying lab. I hope this information is helpful to you. If you are in need of any other product that you might require for your experiments (e.g. secondary antibodies, controls), please let me know as well. Please do not hesitate to contact us if you need any more advice or information.