Anti-GCN5p 抗体 (ab63810)
Key features and details
- Rabbit polyclonal to GCN5p
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
製品の概要
-
製品名
Anti-GCN5p antibody -
製品の詳細
Rabbit polyclonal to GCN5p -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Saccharomyces cerevisiae -
免疫原
Recombinant fragment corresponding to the N-terminal domain (1-300aa) of Saccharomyces cerevisiae GCN5p.
-
ポジティブ･コントロール
- Saccharomyces cerevisiae extract.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6
Preservative: 0.1% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63810の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).
|
| 特記事項 |
|---|
|
WB
1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa). |
ターゲット情報
-
関連性
GCN5p is a histone acetyltransferase that contains a bromo domain and an N-acetyltransferase domain. It is recruited by a number of DNA-binding transcriptional activators, such as GCN4p and HAP2/3/4. GCN5p is a component of a number of different histone acetylation (HAT) complexes, namely, SAGA, SALSA and ADA. The SAGA complex, which is around 1.8 MDa in size, is required for the recruitment of the basal transcriptional machinery to around 10% of RNA polymerase II gene promoters in yeast. The activity of GCN5 may be dependent on its interactions within these multisubunit complexes. GCN5p acetylates histone H2B on K11 and K16 and histone H3 on K9, K14, K18, K23, K27 and K36. It also contributes to acetylation of histone H4 (at K8 and K16) and histone H2A.Z. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 853167 Saccharomyces cerevisiae
- SwissProt: Q03330 Saccharomyces cerevisiae
-
別名
- ADA4 antibody
- GCN 5p antibody
- GCN5 antibody
see all
画像
-
Western blot - Anti-GCN5p antibody (ab63810)Anti-GCN5p antibody (ab63810) at 1/500 dilution + Lysate from S. cerevisiae(BY4741)
Secondary
HRP conjugated Goat anti-Rabbit IgG at 1/5000 dilution
Predicted band size: 51 kDa -
Western blot - Anti-GCN5p antibody (ab63810)All lanes : Anti-GCN5p antibody (ab63810) at 1/5000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Saccharomyces cerevisiae extract
Predicted band size: 51 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Additional bands at: 34 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab63810 は 1 報の論文で使用されています。
- Bian C et al. Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylation. EMBO J 30:2829-42 (2011). WB ; Saccharomyces cerevisiae . PubMed: 21685874