Anti-GCN2 (phospho T667) 抗体 [EPR2319Y] (ab68427)
![Western blot - Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)](https://www.abcam.co.jp/ps/products/68/ab68427/Images/ab68427-82674-anti-gcn2-phospho-t667-antibody-epr2319y-western-blot.gif "Western blot - Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2319Y] to GCN2 (phospho T667)
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GCN2 (phospho T667) antibody [EPR2319Y]
GCN2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2319Y] to GCN2 (phospho T667) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HeLa cells IP: HeLa cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2319Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68427の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/2000. Detects a band of approximately 220 kDa (predicted molecular weight: 220 kDa).
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| IP |
1/30.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/2000. Detects a band of approximately 220 kDa (predicted molecular weight: 220 kDa). |
|
IP
1/30. |
ターゲット情報
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機能
Can phosphorylate the alpha subunit of EIF2 and may mediate translational control. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 protein kinase domains.
Contains 1 RWD domain. -
ドメイン
Kinase domain 1 is a degenerate kinase domain.
RWD domain is reported to interact with GCN1L1. -
翻訳後修飾
Autophosphorylated on threonine residues. - Information by UniProt
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参照データベース
- Entrez Gene: 440275 Human
- Omim: 609280 Human
- SwissProt: Q9P2K8 Human
- Unigene: 656673 Human
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別名
- E2AK4_HUMAN antibody
- Eif2ak4 antibody
- Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody
see all
画像
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Western blot - Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)All lanes : Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427) at 1/2000 dilution
Lane 1 : HeLa cell lysate - untreated
Lane 2 : HeLa cell lysate - treated with LP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution
Predicted band size: 220 kDa
Observed band size: 220 kDa
Additional bands at: 180 kDa. We are unsure as to the identity of these extra bands. -
![Immunoprecipitation - Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)](https://www.abcam.co.jp/ps/products/68/ab68427/Images/ab68427-3-anti-gcn2-phospho-t667-antibody-epr2319y-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)GCN2 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab68427 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: ab68427 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab68427 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)](https://www.abcam.co.jp/ps/products/68/ab68427/Images/ab68427-2-benefits-of-recombinant-antibodies.png)
Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab68427 は 1 報の論文で使用されています。
- Zhong H et al. African Swine Fever Virus MGF110-7L Induces Host Cell Translation Suppression and Stress Granule Formation by Activating the PERK/PKR-eIF2α Pathway. Microbiol Spectr 10:e0328222 (2022). PubMed: 36377947