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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-GCN2 抗体 [EPR25345-125] (ab302609)

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Western blot - Anti-GCN2 antibody [EPR25345-125] (AB302609)
  • Western blot - Anti-GCN2 antibody [EPR25345-125] (AB302609)
  • Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609)
  • Anti-GCN2 antibody [EPR25345-125] (AB302609)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25345-125] to GCN2
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-GCN2 antibody [EPR25345-125]
    GCN2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25345-125] to GCN2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt (Intra),ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Whole cell lysates: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell line), MEF (mouse embryonic fibroblast (immortalized)), NIH/3T3 (mouse embryonic fibroblast), Neuro-2a (mouse neuroblastoma neuroblast), MOLT-4 (human lymphoblastic leukemia T lymphoblast), C6 (rat glial tumor glial cell).

  • 特記事項

    ab302609 does not react in: IHC-P with human, mouse, and rat; ICC with human and mouse; flow cyt. intr. with human and mouse; immunoprecipitation with mouse. 

     

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302609の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 186 kDa (predicted molecular weight: 187 kDa).
特記事項
WB
1/1000. Detects a band of approximately 186 kDa (predicted molecular weight: 187 kDa).
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    Can phosphorylate the alpha subunit of EIF2 and may mediate translational control.

  • 組織特異性

    Widely expressed.

  • 配列類似性

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 2 protein kinase domains.
    Contains 1 RWD domain.

  • ドメイン

    Kinase domain 1 is a degenerate kinase domain.
    RWD domain is reported to interact with GCN1L1.

  • 翻訳後修飾

    Autophosphorylated on threonine residues.
  • Information by UniProt

  • 参照データベース

    • 別名

      • E2AK4_HUMAN antibody
      • Eif2ak4 antibody
      • Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody
      • Eukaryotic translation initiation factor 2-alpha kinase 4 antibody
      • GCN2 antibody
      • GCN2 eIF2alpha kinase antibody
      • GCN2 like protein antibody
      • GCN2-like protein antibody
      • KIAA1338 antibody
      • MGCN2 antibody
      see all

    画像

    • Western blot - Anti-GCN2 antibody [EPR25345-125] (AB302609)
      Western blot - Anti-GCN2 antibody [EPR25345-125] (AB302609)
      Anti-GCN2 antibody [EPR25345-125] (ab302609) at 1/1000 dilution + C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 187 kDa
      Observed band size: 186 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • Western blot - Anti-GCN2 antibody [EPR25345-125] (AB302609)
      Western blot - Anti-GCN2 antibody [EPR25345-125] (AB302609)
      All lanes : Anti-GCN2 antibody [EPR25345-125] (ab302609) at 1/1000 dilution

      Lane 1 : MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate
      Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
      Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
      Lane 4 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 187 kDa
      Observed band size: 186 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    • Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609)
      Western blot - Anti-GCN2 antibody [EPR25345-125] (ab302609)
      All lanes : Anti-GCN2 antibody [EPR25345-125] (ab302609) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell line), whole cell lysate
      Lane 2 : EIF2AK4 (GCN2) KO HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 187 kDa
      Observed band size: 186 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    • Anti-GCN2 antibody [EPR25345-125] (AB302609)
      Anti-GCN2 antibody [EPR25345-125] (AB302609)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302609 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302609 は論文での使用が確認できていません。

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