Anti-GCLM 抗体 (ab154017)
Key features and details
- Rabbit polyclonal to GCLM
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-GCLM antibody
GCLM 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GCLM -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
-
免疫原
Synthetic peptide, corresponding to a region within amino acids 29-120 of Human GCLM (Uniprot ID: P48507).
-
ポジティブ･コントロール
- H1299, 293T, A431, HeLa, HepG2, MOLT4 and Raji whole cell lysates; Human BT474 xenograft tissue; HeLa cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154017の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/1000. |
ターゲット情報
-
組織特異性
In all tissues examined. Highest levels in skeletal muscle. -
パスウェイ
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. -
配列類似性
Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily. - Information by UniProt
-
参照データベース
- Entrez Gene: 525659 Cow
- Entrez Gene: 2730 Human
- Entrez Gene: 14630 Mouse
- Entrez Gene: 29739 Rat
- Omim: 601176 Human
- SwissProt: Q2T9Y6 Cow
- SwissProt: P48507 Human
- SwissProt: O09172 Mouse
see all -
別名
- Gamma ECS regulatory subunit antibody
- Gamma-ECS regulatory subunit antibody
- Gamma-glutamylcysteine synthetase regulatory subunit antibody
see all
画像
-
Western blot - Anti-GCLM antibody (ab154017)Anti-GCLM antibody (ab154017) at 1/5000 dilution + HT1299 whole cell lysate at 30 µg
Predicted band size: 31 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody (ab154017)Immunohistochemical analysis of paraffin-embedded Human BT474 xenograft tissue labeling GCML with ab154017 at 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-GCLM antibody (ab154017)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling GCLM with ab154017 at 1/200 dilution. Lower image is merged with DNA probe.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab154017 は 1 報の論文で使用されています。
- Monti P et al. Mutant p53K120R expression enables a partial capacity to modulate metabolism. Front Genet 13:974662 (2022). PubMed: 36226181