Anti-GCLM 抗体 (ab153967)
Key features and details
- Rabbit polyclonal to GCLM
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-GCLM antibody
GCLM 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GCLM -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow
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免疫原
Recombinant fragment, corresponding to a region within amino acids 1-261 Human GCLM (UniProt: P48507).
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ポジティブ･コントロール
- Human A431, Mouse C8D30 and Rat liver lysates; HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153967の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 31 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 31 kDa. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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組織特異性
In all tissues examined. Highest levels in skeletal muscle. -
パスウェイ
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. -
配列類似性
Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily. - Information by UniProt
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参照データベース
- Entrez Gene: 525659 Cow
- Entrez Gene: 2730 Human
- Entrez Gene: 14630 Mouse
- Entrez Gene: 29739 Rat
- Omim: 601176 Human
- SwissProt: Q2T9Y6 Cow
- SwissProt: P48507 Human
- SwissProt: O09172 Mouse
see all -
別名
- Gamma ECS regulatory subunit antibody
- Gamma-ECS regulatory subunit antibody
- Gamma-glutamylcysteine synthetase regulatory subunit antibody
see all
画像
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Western blot - Anti-GCLM antibody (ab153967)Anti-GCLM antibody (ab153967) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 31 kDa
12% SDS PAGE -
Western blot - Anti-GCLM antibody (ab153967)Anti-GCLM antibody (ab153967) at 1/1000 dilution + C8D30 whole cell lysate at 30 µg
Predicted band size: 31 kDa
12% SDS PAGE -
Western blot - Anti-GCLM antibody (ab153967)Anti-GCLM antibody (ab153967) at 1/1000 dilution + Rat liver lysate
at 50 µg
Predicted band size: 31 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-GCLM antibody (ab153967)Immunofluorescent analysis of methanol-fixed HeLa cells labeling GCLM with ab153967 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab153967 は 4 報の論文で使用されています。
- Buitrago-Molina LE et al. p53-Independent Induction of p21 Fails to Control Regeneration and Hepatocarcinogenesis in a Murine Liver Injury Model. Cell Mol Gastroenterol Hepatol 11:1387-1404 (2021). PubMed: 33484913
- Zhang Y & Li Y Bladder cancer cells prevent cisplatin-induced oxidative stress by upregulating Nestin1 expression. Am J Transl Res 13:11178-11193 (2021). PubMed: 34786050
- Hughey CC et al. Dysregulated transmethylation leading to hepatocellular carcinoma compromises redox homeostasis and glucose formation. Mol Metab 23:1-13 (2019). PubMed: 30850319
- Liang Y et al. Astragaloside IV and ferulic acid synergistically promote neurite outgrowth through Nrf2 activation. Mech Ageing Dev 180:70-81 (2019). PubMed: 30978363