Anti-GCLC 抗体 [EPR13476(B)] (ab181839)
![Western blot - Anti-GCLC antibody [EPR13476(B)] (ab181839)](https://www.abcam.co.jp/ps/products/181/ab181839/Images/ab181839-212740-ab181839WB.jpg "Western blot - Anti-GCLC antibody [EPR13476(B)] (ab181839)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13476(B)] to GCLC
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-GCLC antibody [EPR13476(B)]
GCLC 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13476(B)] to GCLC -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- HepG2, A549 and Human fetal liver lysates.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13476(B) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181839の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa). |
ターゲット情報
-
パスウェイ
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. -
関連疾患
Defects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450]. -
配列類似性
Belongs to the glutamate--cysteine ligase type 3 family. - Information by UniProt
-
参照データベース
- Entrez Gene: 2729 Human
- Entrez Gene: 25283 Rat
- Omim: 606857 Human
- SwissProt: P48506 Human
- SwissProt: P19468 Rat
- Unigene: 654465 Human
- Unigene: 8365 Rat
-
別名
- EC 6.3.2.2 antibody
- Gamma ECS antibody
- Gamma glutamylcysteine synthetase antibody
see all
画像
-
Western blot - Anti-GCLC antibody [EPR13476(B)] (ab181839)Anti-GCLC antibody [EPR13476(B)] (ab181839) at 1/5000 dilution + HepG2 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa -
![Western blot - Anti-GCLC antibody [EPR13476(B)] (ab181839)](https://www.abcam.co.jp/ps/products/181/ab181839/Images/ab181839-212739-ab181839WBb.jpg)
Western blot - Anti-GCLC antibody [EPR13476(B)] (ab181839)All lanes : Anti-GCLC antibody [EPR13476(B)] (ab181839) at 1/10000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa -
![Anti-GCLC antibody [EPR13476(B)] (ab181839)](https://www.abcam.co.jp/ps/products/181/ab181839/Images/ab181839-3-benefits-of-recombinant-antibodies.png)
Anti-GCLC antibody [EPR13476(B)] (ab181839)
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (1)
ab181839 は 1 報の論文で使用されています。
- Lin CH et al. Glutamate-cysteine ligase catalytic subunit as a therapeutic target in acute myeloid leukemia and solid tumors. Am J Cancer Res 11:2911-2927 (2021). PubMed: 34249435