AB181839

Anti-GCLC 抗体 [EPR13476(B)]

Name: Anti-GCLC 抗体 [EPR13476(B)] (ab181839) | Abcam
Brand: Abcam Limited
SKU: AB181839
Price: 54000 JPY
Availability: InStock
Rating: 3 (1 reviews)

Anti-GCLC antibody [EPR13476(B)]

RabMAb
Recombinant
詳細を見る

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal GCLC antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

別名を表示する

GLCL, GLCLC, GCLC, Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase

2 Images

Supplier Data

Western blot - Anti-GCLC antibody [EPR13476(B)] (AB181839)

All lanes:

Western blot - Anti-GCLC antibody [EPR13476(B)] (ab181839) at 1/5000 dilution

All lanes:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

Supplier Data

Western blot - Anti-GCLC antibody [EPR13476(B)] (AB181839)

All lanes:

Western blot - Anti-GCLC antibody [EPR13476(B)] (ab181839) at 1/10000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR13476(B)

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis.

See full target information GCLC

style

left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Microbial cell (Graz, Austria) 10:157-169 PubMed37545643

2023

Caspase 3 exhibits a yeast metacaspase proteostasis function that protects mitochondria from toxic TDP43 aggregates.

Applications

Unspecified application

Species

Unspecified reactive species

Steve Brunette,Anupam Sharma,Ryan Bell,Lawrence Puente,Lynn A Megeney

Neurochemical research 48:2542-2551 PubMed37061657

2023

Dithiolethiones D3T and ACDT Protect Against Iron Overload-Induced Cytotoxicity and Serve as Ferroptosis Inhibitors in U-87 MG Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Neha Kulkarni,Rajitha Gadde,Swati Betharia

American journal of cancer research 11:2911-2927 PubMed34249435

2021

Glutamate-cysteine ligase catalytic subunit as a therapeutic target in acute myeloid leukemia and solid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Chiou-Hong Lin,John P Vu,Chen-Yen Yang,Mint Sirisawad,Chun-Te Chen,Hung Dao,Jing Liu,Xuan Ma,Chin Pan,Joseph Cefalu,Chris Tse,Erica Jackson,Hsu-Ping Kuo

View all publications

style

left-column

Anti-GCLC 抗体 [EPR13476(B)]

Anti-GCLC antibody [EPR13476(B)]

Western blot - Anti-GCLC antibody [EPR13476(B)] (AB181839)

Western blot - Anti-GCLC antibody [EPR13476(B)] (AB181839)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (3)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise