AB236387

Anti-GCH1 抗体 [OTI5A1]

Name: Anti-GCH1 抗体 [OTI5A1] (ab236387) | Abcam
Brand: Abcam Limited
SKU: AB236387
Price: 74000 JPY
Availability: InStock

Anti-GCH1 antibody [OTI5A1]

Be the first to review this product! Submit a review

(6 Publications)

Mouse Monoclonal GCH1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GCH1.

別名を表示する

DYT5, GCH, GCH1, GTP cyclohydrolase 1, GTP cyclohydrolase I, GTP-CH-I

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (AB236387)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (AB236387)

Paraffin-embedded human skin tissue stained for GCH1 using ab236387 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (AB236387)

Paraffin-embedded human placenta tissue stained for GCH1 using ab236387 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (AB236387)

Paraffin-embedded human spleen carcinoma tissue stained for GCH1 using ab236387 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-GCH1 antibody [OTI5A1] (AB236387)

All lanes:

Western blot - Anti-GCH1 antibody [OTI5A1] (ab236387) at 1/2000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY GCH1 cDNA for 48 hrs at 5 µg

Predicted band size: 28 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

OTI5A1

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

Recombinant Full Length Protein corresponding to Human GCH1.

P30793

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

精製に関する特記事項

Purified from cell culture supernatant.

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Positively regulates nitric oxide synthesis in umbilical vein endothelial cells (HUVECs). May be involved in dopamine synthesis. May modify pain sensitivity and persistence. Isoform GCH-1 is the functional enzyme, the potential function of the enzymatically inactive isoforms remains unknown.

See full target information GCH1

style

left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 73:103207 PubMed38805974

2024

Redistribution of defective mitochondria-mediated dihydroorotate dehydrogenase imparts 5-fluorouracil resistance in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shuohui Dong,Mingguang Zhang,Zhiqiang Cheng,Xiang Zhang,Weili Liang,Songhan Li,Linchuan Li,Qian Xu,Siyi Song,Zitian Liu,Guangwei Yang,Xiang Zhao,Ze Tao,Shuo Liang,Kexin Wang,Guangyong Zhang,Sanyuan Hu

Methods in molecular biology (Clifton, N.J.) 2712:211-222 PubMed37578709

2023

Detection of Ferroptosis by Immunohistochemistry and Immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhong,Ruochan Chen

Journal of oncology 2023:1453739 PubMed36793373

2023

Suppression of GCH1 Sensitizes Ovarian Cancer and Breast Cancer to PARP Inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Siyuan Wang,Yu Xia,Pu Huang,Cheng Xu,Yiyu Qian,Tian Fang,Qinglei Gao

Frontiers in cell and developmental biology 10:810327 PubMed35223839

2022

Blockade of GCH1/BH4 Axis Activates Ferritinophagy to Mitigate the Resistance of Colorectal Cancer to Erastin-Induced Ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Hu,Wanhui Wei,Daiqian Wu,Fengxing Huang,Mengting Li,Wenjie Li,Jingwen Yin,Yanan Peng,Yuanyuan Lu,Qiu Zhao,Lan Liu

Molecular medicine reports 18:3350-3358 PubMed30085331

2018

Exendin‑4 reverses endothelial dysfunction in mice fed a high‑cholesterol diet by a GTP cyclohydrolase‑1/tetrahydrobiopterin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqi Tang,Lijuan Liu,Yujie Guo,Guoxiong Deng,Meixiang Chen,Jinru Wei

International journal of cancer 132:591-604 PubMed22753274

2012

Inhibition of GTP cyclohydrolase attenuates tumor growth by reducing angiogenesis and M2-like polarization of tumor associated macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Geethanjali Pickert,Hee-Young Lim,Andreas Weigert,Annett Häussler,Thekla Myrczek,Maximilian Waldner,Sandra Labocha,Nerea Ferreirós,Gerd Geisslinger,Jörn Lötsch,Christoph Becker,Bernhard Brüne,Irmgard Tegeder

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GCH1 抗体 [OTI5A1]

Anti-GCH1 antibody [OTI5A1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (AB236387)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (AB236387)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (AB236387)

Western blot - Anti-GCH1 antibody [OTI5A1] (AB236387)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (6)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise