Anti-GCET2 抗体 [EPR14333] (ab185231)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET2 antibody [EPR14333] (ab185231)](https://www.abcam.co.jp/ps/products/185/ab185231/Images/ab185231-218774-anti-gcet2-antibody-epr14333-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET2 antibody [EPR14333] (ab185231)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14333] to GCET2
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-GCET2 antibody [EPR14333]
GCET2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14333] to GCET2 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- Human Non Hodgkin's lymphoma and Human diffuse large B-cell lymphoma tissues; Raji cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR14333 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185231の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/230.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/500.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/230. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/500. |
ターゲット情報
-
機能
Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation. -
組織特異性
Expressed in diffuse large B-cell lymphoma (DLBCL) and several germinal center (GC)-like lymphoma cell lines (at protein level). Highly expressed in normal GC lymphocytes and GC-derived malignancies. Expressed in thymus and spleen. -
翻訳後修飾
Phosphorylation on tyrosine residues can be induced by IL6. Phosphorylation is mediated by LYN. -
細胞内局在
Cytoplasm. Cell membrane. It relocalizes from the cytoplasm to podosome-like structures upon cell treatment with IL6. - Information by UniProt
-
参照データベース
- Entrez Gene: 257144 Human
- Omim: 607792 Human
- SwissProt: Q8N6F7 Human
- Unigene: 49614 Human
-
別名
- GCAT2 antibody
- Gcet antibody
- GCET2 germinal center expressed transcript 2 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET2 antibody [EPR14333] (ab185231)
Immunohistochemical analysis of paraffin embedded Human diffuse large B-cell lymphoma tissue labeling GCET2 with ab185231 at 1/2000.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Flow Cytometry (Intracellular) - Anti-GCET2 antibody [EPR14333] (ab185231)](https://www.abcam.co.jp/ps/products/185/ab185231/Images/ab185231-218776-anti-gcet2-antibody-epr14333-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-GCET2 antibody [EPR14333] (ab185231)Intracellular flow cytometrical analysis of Raji cells labeling GCET2 with ab185231 at 1/230 and Goat anti rabbit IgG (FITC) at 1/150 as a secondary antibody compared to a negative control antibody.
-
![Immunocytochemistry/ Immunofluorescence - Anti-GCET2 antibody [EPR14333] (ab185231)](https://www.abcam.co.jp/ps/products/185/ab185231/Images/ab185231-218775-anti-gcet2-antibody-epr14333-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GCET2 antibody [EPR14333] (ab185231)Immunofluorescent analysis of Raji cells labeling GCET2 with ab185231 at 1/500 (red) and DAPI staining (blue). Cells fixed in -20℃ Acetone.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET2 antibody [EPR14333] (ab185231)](https://www.abcam.co.jp/ps/products/185/ab185231/Images/ab185231-218773-anti-gcet2-antibody-epr14333-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET2 antibody [EPR14333] (ab185231)Immunohistochemical analysis of paraffin embedded Human Non Hodgkin's lymphoma tissue labeling GCET2 with ab185231 at 1/2000.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Anti-GCET2 antibody [EPR14333] (ab185231)](https://www.abcam.co.jp/ps/products/185/ab185231/Images/ab185231-5-benefits-of-recombinant-antibodies.png)
Anti-GCET2 antibody [EPR14333] (ab185231)
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab185231 は論文での使用が確認できていません。