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Anti-GCET1 抗体 [RAM341] (ab68889)

Submit a review Q&A (5)References (13)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET1 antibody [RAM341] (ab68889)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET1 antibody [RAM341] (ab68889)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET1 antibody [RAM341] (ab68889)

Key features and details

  • Mouse monoclonal [RAM341] to GCET1
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-GCET1 antibody [RAM341]
    GCET1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [RAM341] to GCET1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Pmore details
    適用なし: IHC-Fr

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein corresponding to Human GCET1.

  • ポジティブ･コントロール

    • IHC-P: Human Burkitt's Lymphoma, Follicular B Cell Lymphoma, Diffuse Large B Cell Lymphoma and Lymphocyte Predominant Hodgkin's Lymphoma tissue. Human tonsil tissue.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab68889の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100.
特記事項
IHC-P
1/100.
追加情報
Is unsuitable for IHC-Fr.

ターゲット情報

  • 機能

    Protease inhibitor that inhibits trypsin and trypsin-like serine proteases (in vitro). Inhibits plasmin and thrombin with lower efficiency (in vitro).

  • 組織特異性

    Highly expressed in normal germinal center (GC) B-cells and GC B-cell-derived malignancies.

  • 配列類似性

    Belongs to the serpin family.

  • 細胞内局在

    Cytoplasm; Secreted and Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Centerin antibody
      • GCET1 antibody
      • Germinal center B cell expressed transcript 1 antibody
      • Germinal center B-cell-expressed transcript 1 protein antibody
      • Seprin A9 antibody
      • Serine proteinase inhibitor A11 antibody
      • Serpin A9 antibody
      • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 9 antibody
      • Serpin peptidase inhibitor clade A member 9 antibody
      • SERPINA11 antibody
      • SERPINA11b antibody
      • Serpina9 antibody
      • SPA9_HUMAN antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET1 antibody [RAM341] (ab68889)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET1 antibody [RAM341] (ab68889)
      Ab68889 staining human GCET1 in Burkitt's Lymphoma (A), Follicular B Cell Lymphoma (B), Diffuse Large B Cell Lymphoma (C) and Lymphocyte Predominant Hodgkin's Lymphoma (D) by immunohistochemistry using formalin fixed, paraffin embedded tissue.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET1 antibody [RAM341] (ab68889)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET1 antibody [RAM341] (ab68889)

      IHC image of GCET1 staining in human tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68889, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET1 antibody [RAM341] (ab68889)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCET1 antibody [RAM341] (ab68889)
      Ab68889 staining human GCET1 in human tonsil by immunohistochemistry using paraffin embedded tissue.

    参考文献 (13)

    ab68889 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab68889 は 13 報の論文で使用されています。

    • Perfecto-Avalos Y  et al. Discriminant analysis and machine learning approach for evaluating and improving the performance of immunohistochemical algorithms for COO classification of DLBCL. J Transl Med 17:198 (2019). PubMed: 31185999
    • Tessier-Cloutier B  et al. Cell of origin in diffuse large B-cell lymphoma in systemic lupus erythematosus: molecular and clinical factors associated with survival. Lupus Sci Med 6:e000324 (2019). PubMed: 31205728
    • Boltežar L  et al. Comparison of the algorithms classifying the ABC and GCB subtypes in diffuse large B-cell lymphoma. Oncol Lett 15:6903-6912 (2018). PubMed: 29731865
    • Menter T  et al. Comprehensive phenotypic characterization of PTLD reveals potential reliance on EBV or NF-κB signalling instead of B-cell receptor signalling. Hematol Oncol 35:187-197 (2017). PubMed: 26799990
    • Lu TX  et al. Epstein-Barr virus positive diffuse large B-cell lymphoma predict poor outcome, regardless of the age. Sci Rep 5:12168 (2015). IHC ; Human . PubMed: 26202875
    View all Publications for this product

    レビューと Q&A

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    1-5 of 5 Abreviews or Q&A

    Question

    Hope this e-mail finds you well!



    Kindly confirm if Anti-GCET1 antibodies (https://www.abcam.com/GCET1-antibody-RAM341-ab68889.html & https://www.abcam.com/GCET1-antibody-ab86673.html) of Abcam are validated for Ventana, as enquired by the customer.



    Looking forward towards your valued response on it.

    Read More
    Answer

    Thank you for contacting us.

    Unfortunately, we do not validate our antibodies on any automated system.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Inquiry: Is your Anti-GCET1 antibody an IVD?

    Read More
    Answer

    Thank you very much for contacting us with your question.

    Both of our GCET1 antibodies, Ab68889 and Ab86673, are research use only antibodies and are not approved for diagnostic usage.

    I hope that this information will be useful, but if you have any further questions or need anything else, please let me know and I'll be happy to help.

    Read More

    Question

    I’m in AB4587 and don’t see a link to MSDS.  I see the Data sheet link to the right, but no link to msds. Could you look at this page and tell me where it is?   https://www.abcam.com/Alzheimer-brain-human-temporal-lobe-tissue-slides-ab4587.html    

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    Answer

    Thanks for your email. ab4587 does not have any required MSDS information, so a link will not be present on this datasheet. For example, ab7254 does have a link to MSDS information. I hope this helps. Please contact me again if you have any further questions. 

    Read More

    Question

        I’m looking for MSDS for the items below. B-Amyloid Control Slides (5 Slides/PK) ABCAM AB4587 BCL2 Antibody ABCAM AB32124 BETA Amyloid ABCAM   BLIMP-1 ABCAM AB81961 FOXP3 ANTIBODY (236A/E7) ABCAM WIHC031 GCET1 ABCAM AB68889 GPNMB Antibody (Monoclonal) (100ug) ABCAM AB56584 Human Milk Fat Globule 1 Antibody  (500 uL) ABCAM AB17787 IMP-3 antibody ABCAM AB82044 Mesothelioma Positive Controls ABCAM AB4437 Oct1 Antibody (500uL) ABCAM AB15112 SMOOTHELIN ANTIBODY ABCAM   TFE-3 ABCAM AB70008 Ubiquitin ABCAM AB7254      

    Read More
    Answer

    Thanks for your enquiry. If required, MSDS information is located via one or more 'MSDS' links near the top left corner of the online datasheet. This link is just below the 'Datasheet PDF' link present on all datasheets. I hope this is helpful. Please contact me again if you have any further questions.

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    Question

     We have purchased GCET1 (ab68889).  We need to know the expiration date for this antibody.  It is not indicated on the vial or the accompanying paperwork.

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    Answer

    Thank you for contacting us. We do not have expiration dates for our products. But we do guarantee all our products for tested species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise. The recommended longterm storage for the antibody is -20 or -80 degree. Therefore the product should be viable for up to 5 years. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com