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リコンビナントRabMAb

Anti-GC1q R 抗体 [EPR23238-6] (ab270033)

Submit a review Submit a question References (1)
Western blot - Anti-GC1q R antibody [EPR23238-6] (ab270033)
  • Western blot - Anti-GC1q R antibody [EPR23238-6] (ab270033)
  • Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-6] (ab270033)
  • Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-6] (ab270033)
  • Flow Cytometry (Intracellular) - Anti-GC1q R antibody [EPR23238-6] (ab270033)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-6] (ab270033)
  • Anti-GC1q R antibody [EPR23238-6] (ab270033)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23238-6] to GC1q R
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-GC1q R antibody [EPR23238-6]
    GC1q R 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23238-6] to GC1q R

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details
    適用なし: IP

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, A549, MCF7 and WI-38 cell lysate; Human lung cancer and spleen tissue lysate. IHC-P: Human cerebral cortex tissue. ICC/IF: A549 and HeLa cells. Flow: HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR23238-6

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab270033の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/600.
ICC/IF
1/500.
WB
1/1000. Predicted molecular weight: 31 kDa.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/600.
ICC/IF
1/500.
WB
1/1000. Predicted molecular weight: 31 kDa.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    Binds to the globular "heads" of C1Q thus inhibiting C1 activation.

  • 配列類似性

    Belongs to the MAM33 family.

  • 細胞内局在

    Mitochondrion matrix. Nucleus. Might also be nuclear in some cell types.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ASF/SF2 associated protein p32 antibody
      • C1q globular domain binding protein antibody
      • C1qBP antibody
      • C1QBP_HUMAN antibody
      • Complement component 1 q subcomponent binding protein antibody
      • Complement component 1 Q subcomponent binding protein mitochondrial antibody
      • Complement component 1 Q subcomponent-binding protein, mitochondrial antibody
      • GC1Q R antibody
      • GC1q R protein antibody
      • GC1q-R protein antibody
      • GC1QBP antibody
      • GC1QR antibody
      • globular domain of, C1q, receptor for antibody
      • Glycoprotein gC1qBP antibody
      • HABP 1 antibody
      • HABP1 antibody
      • Hyaluronan binding protein 1 antibody
      • Hyaluronan-binding protein 1 antibody
      • Mitochondrial matrix protein p32 antibody
      • p32 antibody
      • p32 splicing factor antibody
      • p33 antibody
      • Pre mrna splicing factor SF2 P32 subunit precursor antibody
      • SF2p32 antibody
      • Splicing factor SF2 associated protein antibody
      see all

    画像

    • Western blot - Anti-GC1q R antibody [EPR23238-6] (ab270033)
      Western blot - Anti-GC1q R antibody [EPR23238-6] (ab270033)
      All lanes : Anti-GC1q R antibody [EPR23238-6] (ab270033) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : A549 (human lung carcinoma epithelial cell) whole cell lysate
      Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
      Lane 4 : WI-38 ( human fetal lung fibroblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 31 kDa



      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20100866).

      Blocking/diluting buffer and concentration: 5% NFDM/TBST.

      Exposure time: Lanes 1-3: 15 secs; Lane 4: 3 secs.

    • Western blot - Anti-GC1q R antibody [EPR23238-6] (ab270033)
      Western blot - Anti-GC1q R antibody [EPR23238-6] (ab270033)
      All lanes : Anti-GC1q R antibody [EPR23238-6] (ab270033) at 1/1000 dilution

      Lane 1 : Human lung cancer tissue lysate
      Lane 2 : Human spleen tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Predicted band size: 31 kDa



      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20100866).

      Blocking/diluting buffer and concentration: 5% NFDM/TBST.

      Exposure time: 15 secs.

    • Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-6] (ab270033)
      Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-6] (ab270033)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 cells labeling GC1q R with ab270033 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing mitochondrial staining in A549 cell line. ab33985 Anti-COX IV antibody (human) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

      -ve control 1: Stained with ab270033 at 1/500 followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution.

      -ve control 2: Stained with ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution followed by ab33985 Anti-COX IV antibody (human) at 1/1000 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-6] (ab270033)
      Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-6] (ab270033)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling GC1q R with ab270033 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing mitochondrial staining in HeLa cell line. ab33985 Anti-COX IV antibody (human) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

      -ve control 1: Stained with ab270033 at 1/500 followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/1000 diliution.

      -ve control 2: Stained with ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution followed by ab33985 Anti-COX IV antibody (human) at 1/1000 dilution.

    • Flow Cytometry (Intracellular) - Anti-GC1q R antibody [EPR23238-6] (ab270033)
      Flow Cytometry (Intracellular) - Anti-GC1q R antibody [EPR23238-6] (ab270033)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GC1q R with ab270033 at 1/600 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-6] (ab270033)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-6] (ab270033)

      Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling GC1q R with ab270033 at 1/500 (1.27 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebral cortex is observed (PMID: 23924515). The section was incubated with ab270033 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    • Anti-GC1q R antibody [EPR23238-6] (ab270033)
      Anti-GC1q R antibody [EPR23238-6] (ab270033)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab270033 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab270033 は 1 報の論文で使用されています。

    • Kerdidani D  et al. Lung tumor MHCII immunity depends on in situ antigen presentation by fibroblasts. J Exp Med 219:N/A (2022). PubMed: 35029648

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