Anti-GC1q R 抗体 [EPR23238-6]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-GC1q R antibody [EPR23238-6] (AB270033)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GC1q R with ab270033 at 1/600 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-6] (AB270033)

Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling GC1q R with ab270033 at 1/500 (1.27 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebral cortex is observed (PMID : 23924515). The section was incubated with ab270033 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-6] (AB270033)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling GC1q R with ab270033 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing mitochondrial staining in HeLa cell line. ab33985 Anti-COX IV antibody (human) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

-ve control 1 : Stained with ab270033 at 1/500 followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) at a 1/1000 diliution.

-ve control 2 : Stained with ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution followed by ab33985 Anti-COX IV antibody (human) at 1/1000 dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-6] (AB270033)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 cells labeling GC1q R with ab270033 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing mitochondrial staining in A549 cell line. ab33985 Anti-COX IV antibody (human) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

-ve control 1 : Stained with ab270033 at 1/500 followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) at a 1/1000 dilution.

-ve control 2 : Stained with ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution followed by ab33985 Anti-COX IV antibody (human) at 1/1000 dilution.

• WB

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Western blot - Anti-GC1q R antibody [EPR23238-6] (AB270033)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 20100866).

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lanes 1-3 : 15 secs; Lane 4 : 3 secs.

All lanes:

Western blot - Anti-GC1q R antibody [EPR23238-6] (ab270033) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

WI-38 ( human fetal lung fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 31 kDa

false

• WB

Supplier Data

Western blot - Anti-GC1q R antibody [EPR23238-6] (AB270033)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 20100866).

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 15 secs.

All lanes:

Western blot - Anti-GC1q R antibody [EPR23238-6] (ab270033) at 1/1000 dilution

Lane 1:

Human lung cancer tissue lysate at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 31 kDa

false