Anti-GC1q R 抗体 [EPR23238-107] (ab270032)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23238-107] to GC1q R
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GC1q R antibody [EPR23238-107]
GC1q R 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23238-107] to GC1q R -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details
適用なし: IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa, A549, MCF7, WI-38 cell lysate; Rat, human and mouse spleen lysate; Human lung cancer lysate. IHC-P: Human, mouse and rat cerebral cortex. ICC/IF: HeLa and C2C12 cells. Flow: HeLa and C2C12 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23238-107 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab270032の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/600.
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/100.
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WB |
1/1000. Predicted molecular weight: 31 kDa.
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特記事項 |
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Flow Cyt (Intra)
1/600. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/100. |
WB
1/1000. Predicted molecular weight: 31 kDa. |
ターゲット情報
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機能
Binds to the globular "heads" of C1Q thus inhibiting C1 activation. -
配列類似性
Belongs to the MAM33 family. -
細胞内局在
Mitochondrion matrix. Nucleus. Might also be nuclear in some cell types. - Information by UniProt
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参照データベース
- Entrez Gene: 708 Human
- Entrez Gene: 12261 Mouse
- Entrez Gene: 29681 Rat
- Omim: 601269 Human
- SwissProt: Q07021 Human
- SwissProt: O35658 Mouse
- SwissProt: O35796 Rat
- Unigene: 555866 Human
see all -
別名
- ASF/SF2 associated protein p32 antibody
- C1q globular domain binding protein antibody
- C1qBP antibody
see all
画像
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All lanes : Anti-GC1q R antibody [EPR23238-107] (ab270032) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 2 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 31 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 15 secs.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20100866).
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling GC1q R with ab270032 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing mitochondrial staining in HeLa cell line. ab33985 Anti-COX IV antibody (human) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative controls:
-ve control 1: ab270032 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab150077 at 1/1000 dilution followed by ab33985 at 1/1000 dilution. -
Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling GC1q R with ab270032 at 1/2000 (0.248 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebral cortex (PMID: 23924515). The section was incubated with ab270032 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GC1q R with ab270032 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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All lanes : Anti-GC1q R antibody [EPR23238-107] (ab270032) at 1/5000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 3 : MCF7 ( human breast adenocarcinoma epithelial cell) whole cell
Lane 4 : Rat spleen lysate
Lane 5 : Mouse spleen lysate
Lane 6 : WI-38 (human fetal lung fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 31 kDaBlocking/diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lanes 1-5: 26 secs; Lane 6: 10 secs.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20100866).
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All lanes : Anti-GC1q R antibody [EPR23238-107] (ab270032) at 1/5000 dilution
Lane 1 : Human lung cancer lysate
Lane 2 : Human spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 31 kDaBlocking/diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 26 secs.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20100866).
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) cells labeling GC1q R with ab270032 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 cells labeling GC1q R with ab270032 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing mitochondrial staining in C2C12 cell line. ab33985 Anti-COX IV antibody (human) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative controls:
-ve control 1: ab270032 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab150077 at 1/1000 dilution followed by ab33985 at 1/1000 dilution. -
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling GC1q R with ab270032 at 1/2000 (0.248 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebral cortex (PMID: 9414106). The section was incubated with ab270032 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
-
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling GC1q R with ab270032 at 1/2000 (0.248 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebral cortex (PMID: 9414106). The section was incubated with ab270032 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab270032 は 1 報の論文で使用されています。
- Butterworth S et al. Toxoplasma gondii virulence factor ROP1 reduces parasite susceptibility to murine and human innate immune restriction. PLoS Pathog 18:e1011021 (2022). PubMed: 36476844