Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-GC1q R 抗体 [60.11] (ab24733)

Reviews (3)Q&A (3)References (30)
Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [60.11] (ab24733)
  • Western blot - Anti-GC1q R antibody [60.11] (ab24733)
  • Western blot - Anti-GC1q R antibody [60.11] (ab24733)
  • Flow Cytometry (Intracellular) - Anti-GC1q R antibody [60.11] (ab24733)
  • Flow Cytometry (Intracellular) - Anti-GC1q R antibody [60.11] (ab24733)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [60.11] (ab24733)

Key features and details

  • Mouse monoclonal [60.11] to GC1q R
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-GC1q R antibody [60.11]
    GC1q R 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [60.11] to GC1q R

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Recombinant full length protein corresponding to GC1q R.

  • ポジティブ･コントロール

    • This antibody gave a positive signal in Human tonsil tissue sections. This antibody gave a positive signal in the following Methanol fixed cell lines: HeLa.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab24733の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (2)
Use a concentration of 5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt (Intra)
Use 0.5µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
特記事項
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt (Intra)
Use 0.5µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Binds to the globular "heads" of C1Q thus inhibiting C1 activation.

  • 配列類似性

    Belongs to the MAM33 family.

  • 細胞内局在

    Mitochondrion matrix. Nucleus. Might also be nuclear in some cell types.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ASF/SF2 associated protein p32 antibody
    • C1q globular domain binding protein antibody
    • C1qBP antibody
    • C1QBP_HUMAN antibody
    • Complement component 1 q subcomponent binding protein antibody
    • Complement component 1 Q subcomponent binding protein mitochondrial antibody
    • Complement component 1 Q subcomponent-binding protein, mitochondrial antibody
    • GC1Q R antibody
    • GC1q R protein antibody
    • GC1q-R protein antibody
    • GC1QBP antibody
    • GC1QR antibody
    • globular domain of, C1q, receptor for antibody
    • Glycoprotein gC1qBP antibody
    • HABP 1 antibody
    • HABP1 antibody
    • Hyaluronan binding protein 1 antibody
    • Hyaluronan-binding protein 1 antibody
    • Mitochondrial matrix protein p32 antibody
    • p32 antibody
    • p32 splicing factor antibody
    • p33 antibody
    • Pre mrna splicing factor SF2 P32 subunit precursor antibody
    • SF2p32 antibody
    • Splicing factor SF2 associated protein antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [60.11] (ab24733)
    Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [60.11] (ab24733)

    ab24733 staining GC1q R in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab24733 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  • Western blot - Anti-GC1q R antibody [60.11] (ab24733)
    Western blot - Anti-GC1q R antibody [60.11] (ab24733)
    All lanes : Anti-GC1q R antibody [60.11] (ab24733) at 5 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 5 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?
    Additional bands at: 68 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • Western blot - Anti-GC1q R antibody [60.11] (ab24733)
    Western blot - Anti-GC1q R antibody [60.11] (ab24733)This image is courtesy of an anonymous Abreview
    Lane 1 : Mw standards
    Lanes 2-3 : Anti-GC1q R antibody [60.11] (ab24733) at 1/2000 dilution

    Lane 2 : 293T whole cell lysate at 20 µg
    Lane 3 : Hela whole cell lysate at 20 µg

    Secondary
    Lanes 2-3 : Alexa Fluor® 680 conjugated goat anti-mouse

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 32 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    The blot was blocked with 5% milk for 1 hour at 25°C prior to incubating with the primary antibody for 13 hours at 4°C.

    See Abreview

  • Flow Cytometry (Intracellular) - Anti-GC1q R antibody [60.11] (ab24733)
    Flow Cytometry (Intracellular) - Anti-GC1q R antibody [60.11] (ab24733)Image courtesy of David Sanchez Martin by Abreview.
    ab24733 used in Flow Cytometry.
    U937 cells were cultured in RPMI 10% FCS, treated with PMA (5nM) and, 6-12 hours later, cells were harvested, spinned and resuspended to 200,000 cells in 100µl. ab24733 used at 10µg/ml for 30 minutes at 4°C. A phycoerythrin conjugated goat anti-mouse polyclonal was used as the secondary antibody at a 1/100 dilution.

    See Abreview

  • Flow Cytometry (Intracellular) - Anti-GC1q R antibody [60.11] (ab24733)
    Flow Cytometry (Intracellular) - Anti-GC1q R antibody [60.11] (ab24733)

    Overlay histogram showing HeLa cells stained with ab24733 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab24733, 0.5μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150117) at 1/4000 dilution for 30 min at 22°C.

    Isotype control antibody (black line) was mouse IgG1 [15-6E10A7] (ab170190, 0.5μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [60.11] (ab24733)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [60.11] (ab24733)
    IHC image of ab24733 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24733, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献 (30)

ab24733 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab24733 は 30 報の論文で使用されています。

  • Kienzle L  et al. A small protein coded within the mitochondrial canonical gene nd4 regulates mitochondrial bioenergetics. BMC Biol 21:111 (2023). PubMed: 37198654
  • Sun YJ  et al. HABP1 promotes proliferation and invasion of lung adenocarcinoma cells through NFκB pathway. Neoplasma 69:155-164 (2022). PubMed: 34881631
  • Phan QT  et al. Serum bridging molecules drive candidal invasion of human but not mouse endothelial cells. PLoS Pathog 18:e1010681 (2022). PubMed: 35797411
  • Koo BH  et al. Arginase II protein regulates Parkin-dependent p32 degradation that contributes to Ca2+-dependent eNOS activation in endothelial cells. Cardiovasc Res 118:1344-1358 (2022). PubMed: 33964139
  • Wang Z  et al. Circular RNA MTCL1 promotes advanced laryngeal squamous cell carcinoma progression by inhibiting C1QBP ubiquitin degradation and mediating beta-catenin activation. Mol Cancer 21:92 (2022). PubMed: 35366893
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-GC1q R antibody [60.11]

 Inconclusive
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (gradient 4% to 12%)
Sample
Pig Cell lysate - whole cell (PK15)
Specification
PK15
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. XXXXXX XXXXXX

Verified customer

投稿 Jan 23 2015

Flow Cytometry abreview for Anti-GC1q R antibody [60.11]

 Good
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (Human Cell (U-937 (ATCC: CRL-1593.2) histiocytic l)
Specification
Human Cell (U-937 (ATCC: CRL-1593.2) histiocytic l
Preparation
Cell harvesting/tissue preparation method: U937 cells were culture in RPMI 10% FCS, treated with PMA (5nM) and, 6-12 hours later, cells were harvested, spinned and resuspend to 200 000cells in 100ul
Sample buffer: PBS + 0.1% FCS
Fixation
No fixation
Permeabilization
No
Gating Strategy
FS - SS
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. XXXXXX XXXXXX

Verified customer

投稿 Jan 24 2011

Western blot abreview for Anti-GC1q R antibody [60.11]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela and 293T)
Loading amount
20 µg
Specification
Hela and 293T
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 23 2008

Question

Dear ,
Thank you for your kind reply. Further to question3. I am hoping to use this antibody as a neutralizing antibody to deliver intraplantarlyin vivo, and hence I am wondering what will be the best control antibody (which monoclonal IgG1) for this and if it is available through abcam? I will be grateful if you could advise me on this. I will place the order as soon as I get all the information. Thank you again :)
Best regards,

Read More
Answer

Thank you for your reply.

I will recommend you to use the next antibody as an isotype control for Functional Assays:

https://www.abcam.com/Mouse-IgG1-kappa-monoclonal-MG1-45-Isotype-control-ab18447.html

As it is suitable for these kinds of assays it is therefore free of sodium azide.

I hope this helps. If not please let me know and I will be happy to assist you further.

Read More

Question

vielen Dank für Ihre Antwort.
Ich hatte bereits in einem 2. Ansatz eine Antikörper-Konzentration von 5µg/ml eingesetzt, da dass unsere Standardkonzentration bei IPs ist.
Zu Ihrem 2. Punkt: Dieser war in meinen Angaben wohl missverständlich dargestellt. Die Inkubation des Antikörpers mit den Beads erfolgte zunächst ohne Lysat, im 2. Ansatz sogar für 4h.
Das Ergebnis blieb in beiden Fällen allerdings negativ.
Ich habe auf Ihrer Internetseite gesehen, dass es mehrere Antikörper gegen das Produkt gibt. Für einen ist die IP auch mit einer Abbildung bestätigt: ab101267. Vielleicht könnten Sie mir diesen auf Kulanz schicken?

Read More
Answer

Vielen Dank für Ihre Antwort und für diese weiteren Informationen.

Es tut mir leid zu hören, dass unsere Vorschläge in diesem Fall nicht zu einer Verbesserung Ihrer Ergebnisse geführt haben. Ich weiß, dass Sie viel Zeit für diese Experimente verwendet haben, und stelle Ihnen daher gerne den ab101267 Anti-GC1q R antibody als kostenlosen Ersatz aus (vorausgesetzt, das Produkt wurde in den letzten 180 Tagen gekauft). Dafür benötige ich jedoch ihre ursprüngliche Bestellnummer oder das Datum der Bestellung.


Vielen Dank für Ihre Kooperation und Hilfsbereitschaft. Ich freue mich bald wieder von Ihnen zu hören.

Read More

Question

1) Abcam product code ab24733
2) Abcam order reference number or product batch number
3) Description of the problem: no IP
4) Sample preparation:
Type of sample (whole cell lysates, fraction, recombinant protein…): whole Hela cell lysate
Lysis buffer Tris pH8,0; NP40; NaCl
Protease inhibitors: Roche
Phosphatase inhibitors Roche
Amount of Lysate IPed ug or number of cells 4 000 000 cells per IP
Lysate precleared with matrix : no
Positive control: yes
Negative control: yes
5) IP antibody: ab24733
Amount/concentration or dilution used 1:5000
Conjugation:
Antibody noncovalently bound to matrix before incubation with lysate sample?
Antibody-lysate incubation time: over night
Incubation temperature 4°C
6) IP Matrix (e. beads): beads
Type of matrix Dynabeads Protein G and A (Invitrogen)
Amount of matrix 1,5 mg
7) Washing after antibody-lysate incubation:
Buffer Lysisbuffer
Number of washes 5 times
8) IP analysis:
Verification method: e.g reprobe in western blotting: WB
Describe verification method briefly: Input, IP, non-bound fraction
OR if Western blotting reprobe performed please indicate the following:
Reducing agent: Lämmlibuffer (SDS, beta 2-mercaptoethanol)
Boiling for ≥5 min? 5 min
Protein loaded ug/lane or cells/lane 2 000 000 cells per IP, 200 000 cells Input and non-bound fraction
Percentage of gel: 10,5%
Type of membrane: Hybond ECL (GE)
Protein transfer verified yes
Blocking agent and concentration 5% Milk in TBST
Blocking time 1h
Blocking temperature RT
Primary antibody (If more than one was used, describe in “additional notes”) ab24733
Concentration or dilution 1:200
Diluent buffer 5% BSA in TBST
Incubation time over night
Incubation temperature: RT
Secondary antibody: Anti-Mouse IgG-HRP
Species: Mouse
Reacts against: ab24733
Concentration or dilution 1:5000
Diluent buffer 5% Milk in TBST
Incubation time 1h
Incubation temperature: RT
Fluorochrome or enzyme conjugate: HRP
Washing after primary and secondary antibodies:
Buffer: TBST
Number of washes 3
Detection method ECL
Was the method successfully used to detect protein from non-IPed samples? yes
Document attachment: Attaching images of your blot is strongly recommended and can greatly speed up our investigation of your problem.

Read More
Answer

Vielen Dank für Ihre Anfrage und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

Es tut mir leid, dass Sie Probleme mit unserem Antikörper hatten.

Um Ihr Problem zu lösen, möchte ich die folgenden Vorschläge zu Veränderung Ihres Protokolls machen:

1. Mir ist aufgefallen, dass die empfohlene Starkonzentration für all unsere anderen Applikationen 5ug/ml beträgt, und dies ist doch ausgesprochen ungewöhnlich, da IP normalerweise eine höhere Konzentration benötigt als z.B. ein WB. Ich vermute daher, dass es sich hier um einen Datenblattfehler handelt, und habe unser Labor zur Rückmeldung kontaktiert. Ich möchte Ihnen daher empfehlen, die Konzentration ebenfalls auf 5 ug/ml zu erhöhen.

2.Um den Antikörpern die Bindung an die Beads zu erleichtern, möchte ich Vorschlagen, erst die Antikörper an die Beads zu koppeln ( Inkubation für 2h), und dann erst mit dem Lysat zu behandeln.



Bitte lassen Sie mich wissen, ob ich Ihnen helfen konnte. Wir bieten unseren Kunden gerne einen kostenlosen Ersatz, eine Gutschrift oder eine Erstattung an, wenn das Produkt wie auf dem Datenblatt beschrieben behandelt und innerhalb von 180 Tagen nach Erhalt reklamiert wurde.

Zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com