AB250128

Anti-GBP2 抗体 [EPR13206] - BSA and Azide free

Anti-GBP2 antibody [EPR13206] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal GBP2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

Guanylate-binding protein 2, GTP-binding protein 2, Guanine nucleotide-binding protein 2, Interferon-induced guanylate-binding protein 2, GBP-2, HuGBP-2, GBP2

3 Images

Lab

Western blot - Anti-GBP2 antibody [EPR13206] - BSA and Azide free (AB250128)

This data was developed using ab179829, the same antibody clone in a different buffer formulation.

Lanes 1-4 : Merged signal (red and green). Green - ab179829 observed at 67 kDa. Red - loading control ab8245 observed at 36 kDa.

ab179829 Anti-GBP2 antibody [EPR13206] - N-terminal was shown to specifically react with GBP2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267218 (knockout cell lysate ab257962) was used. Wild-type and GBP2 knockout samples were subjected to SDS-PAGE. ab179829 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GBP2 antibody [EPR13206] - N-terminal (<a href='/products/primary-antibodies/gbp2-antibody-epr13206-n-terminal-ab179829'>ab179829</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

GBP2 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human GBP2 knockout A549 cell line (<a href='/products/cell-lines/human-gbp2-knockout-a549-cell-line-ab267218'>ab267218</a>)

Lane 3:

K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate at 20 µg

Lane 4:

HaCaT (Human keratinocyte cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 67 kDa

Observed band size: 67 kDa

false

Lab

Western blot - Anti-GBP2 antibody [EPR13206] - BSA and Azide free (AB250128)

This data was developed using ab179829, the same antibody clone in a different buffer formulation.

Lanes 1-4 : Merged signal (red and green). Green - ab179829 observed at 70 kDa. Red - loading control ab8245 observed at 36 kDa.

ab179829 Anti-GBP2 antibody [EPR13206] - N-terminal was shown to specifically react with GBP2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267219 (knockout cell lysate ab257963) was used. Wild-type and GBP2 knockout samples were subjected to SDS-PAGE. ab179829 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GBP2 antibody [EPR13206] - N-terminal (<a href='/products/primary-antibodies/gbp2-antibody-epr13206-n-terminal-ab179829'>ab179829</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

GBP2 knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human GBP2 knockout A549 cell line (<a href='/products/cell-lines/human-gbp2-knockout-a549-cell-line-ab267219'>ab267219</a>)

Lane 3:

K-562 cell lysate at 20 µg

Lane 4:

HaCaT cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 67 kDa

Observed band size: 70 kDa

false

Supplier Data

Western blot - Anti-GBP2 antibody [EPR13206] - BSA and Azide free (AB250128)

This data was developed using ab179829, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GBP2 antibody [EPR13206] - N-terminal (<a href='/products/primary-antibodies/gbp2-antibody-epr13206-n-terminal-ab179829'>ab179829</a>) at 1/1000 dilution

Lane 1:

Human spleen cell lysate

Lane 2:

IM-9 cell lysate

Lane 3:

HACAT cell lysate

Lane 4:

K562 cell lysate

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 67 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR13206

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

ab250128 is the carrier-free version of ab179829.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GBP2 also known as Guanylate Binding Protein 2 is a member of the dynamin superfamily of large GTPases. It has an approximate mass of 67 kDa. This protein finds expression predominantly in macrophages and other immune cells. The involvement of GBP2 in cellular defense mechanisms reflects its upregulation by interferon-gamma where it takes part in immune responses against microbial and viral infections.

Biological function summary

Guanylate Binding Protein 2 participates in immune response regulation. It does not function as part of a large multiprotein complex but acts primarily as a monomer. GBP2 disrupts pathogenic molecular machinery by binding to microbial enzymes leading to their inhibition which limits pathogen survival. This mechanism ensures that GBP2 remains a significant factor in the cell's intrinsic ability to fight infections at the molecular level.

Pathways

Guanylate Binding Protein 2 plays an important role in the interferon-induced defense pathways. It participates in the immune effector pathways primarily connecting to processes like the JAK-STAT signaling pathway. Through this involvement GBP2 helps enhance the expression of a variety of immunologically relevant genes. Additionally GBP2 often interacts with other members of the GBP family including GBP1 and GBP5 to amplify immune responses and signaling cascades.

Guanylate Binding Protein 2 shows a significant connection to infectious diseases like tuberculosis and viral infections such as hepatitis C. Overexpression or dysregulation of GBP2 can influence the progression and severity of these conditions. Additionally GBP2's interaction with proteins involved in inflammatory responses such as other interferon-induced proteins highlights its influence over auto-inflammatory conditions and its potential as a biomarker for disease progression.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Interferon (IFN)-inducible GTPase that plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens (PubMed : 31091448). Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions, but the major reaction product is GDP (PubMed : 8706832). Following infection, recruited to the pathogen-containing vacuoles or vacuole-escaped bacteria and acts as a positive regulator of inflammasome assembly by promoting the release of inflammasome ligands from bacteria (By similarity). Acts by promoting lysis of pathogen-containing vacuoles, releasing pathogens into the cytosol (By similarity). Following pathogen release in the cytosol, promotes recruitment of proteins that mediate bacterial cytolysis : this liberates ligands that are detected by inflammasomes, such as lipopolysaccharide (LPS) that activates the non-canonical CASP4/CASP11 inflammasome or double-stranded DNA (dsDNA) that activates the AIM2 inflammasome (By similarity). Confers protection to the protozoan pathogen Toxoplasma gondii (By similarity). Independently of its GTPase activity, acts as an inhibitor of various viruses infectivity, such as HIV-1, Zika and influenza A viruses, by inhibiting FURIN-mediated maturation of viral envelope proteins (PubMed : 31091448).

See full target information GBP2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 295:12868-12884 PubMed32580945

2020

Adipose mesenchymal stem cell-derived extracellular vesicles containing microRNA-26a-5p target TLR4 and protect against diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yurui Duan,Qingyang Luo,Yun Wang,Yali Ma,Fang Chen,Xiaoguang Zhu,Jun Shi

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GBP2 抗体 [EPR13206] - BSA and Azide free

Anti-GBP2 antibody [EPR13206] - BSA and Azide free

Western blot - Anti-GBP2 antibody [EPR13206] - BSA and Azide free (AB250128)

Western blot - Anti-GBP2 antibody [EPR13206] - BSA and Azide free (AB250128)

Western blot - Anti-GBP2 antibody [EPR13206] - BSA and Azide free (AB250128)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

組合せ製品

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise