Anti-GBGT1 抗体 [EPR10467] (ab154837)
![Western blot - Anti-GBGT1 antibody [EPR10467] (ab154837)](https://www.abcam.co.jp/ps/products/154/ab154837/Images/ab154837-161422-anti-gbgt1-antibody-epr10467-western-blot.jpg "Western blot - Anti-GBGT1 antibody [EPR10467] (ab154837)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10467] to GBGT1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GBGT1 antibody [EPR10467]
GBGT1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10467] to GBGT1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
適用なし: ICC/IF or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human placenta, ovary cancer, fetal heart and fetal lung lysates; HeLa and Jurkat whole cell lysate (ab7899); Human kidney and pancreas tissues; Jurkat cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR10467 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154837の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Catalyzes the formation of some glycolipid via the addition of N-acetylgalactosamine (GalNAc) in alpha-1,3-linkage to some substrate. Glycolipids probably serve for adherence of some pathogens. -
組織特異性
Widely expressed. Expressed at higher level in placenta, ovary and peripheral blood leukocyte, whereas it is weakly expressed in liver, thymus, and testis. -
パスウェイ
Protein modification; protein glycosylation. -
配列類似性
Belongs to the glycosyltransferase 6 family. -
ドメイン
The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis. -
細胞内局在
Golgi apparatus membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 26301 Human
- Entrez Gene: 227671 Mouse
- Omim: 606074 Human
- SwissProt: Q8N5D6 Human
- SwissProt: Q8VI38 Mouse
- Unigene: 495419 Human
- Unigene: 484270 Mouse
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別名
- 3-N-acetylgalactosaminyltransferase 1 antibody
- A3GALNT antibody
- EC=2.4.1.- antibody
see all
画像
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Western blot - Anti-GBGT1 antibody [EPR10467] (ab154837)All lanes : Anti-GBGT1 antibody [EPR10467] (ab154837) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : Human ovary cancer tissue lysate
Lane 3 : Human fetal heart tissue lysate
Lane 4 : Human fetal lung tissue lysate
Lane 5 : HeLa cell lysate
Lane 6 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBGT1 antibody [EPR10467] (ab154837)](https://www.abcam.co.jp/ps/products/154/ab154837/Images/ab154837-161421-anti-gbgt1-antibody-epr10467-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBGT1 antibody [EPR10467] (ab154837)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling GBGT1 using ab154837 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBGT1 antibody [EPR10467] (ab154837)](https://www.abcam.co.jp/ps/products/154/ab154837/Images/ab154837-161420-anti-gbgt1-antibody-epr10467-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBGT1 antibody [EPR10467] (ab154837)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling GBGT1 using ab154837 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-GBGT1 antibody [EPR10467] (ab154837)](https://www.abcam.co.jp/ps/products/154/ab154837/Images/ab154837-161419-anti-gbgt1-antibody-epr10467-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-GBGT1 antibody [EPR10467] (ab154837)Intracellular Flow Cytometry analysis of permeabilized Jurkat cells labeling GBGT1 (red), using ab154837 at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)
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![Anti-GBGT1 antibody [EPR10467] (ab154837)](https://www.abcam.co.jp/ps/products/154/ab154837/Images/ab154837-5-benefits-of-recombinant-antibodies.png)
Anti-GBGT1 antibody [EPR10467] (ab154837)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab154837 は論文での使用が確認できていません。