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リコンビナントRabMAb

Anti-Gbeta5 抗体 [EPR14779(B)] (ab185214)

Submit a review Submit a question References (1)
Western blot - Anti-Gbeta5 antibody [EPR14779(B)] (ab185214)
  • Western blot - Anti-Gbeta5 antibody [EPR14779(B)] (ab185214)
  • Anti-Gbeta5 antibody [EPR14779(B)] (ab185214)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14779(B)] to Gbeta5
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Gbeta5 antibody [EPR14779(B)]
    Gbeta5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14779(B)] to Gbeta5

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human cerebellum tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR14779(B)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185214の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 44 kDa).
特記事項
WB
1/1000 - 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 44 kDa).

ターゲット情報

  • 機能

    Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

  • 組織特異性

    Expressed in multiple tissues.

  • 配列類似性

    Belongs to the WD repeat G protein beta family.
    Contains 7 WD repeats.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • FLJ37457 antibody
    • FLJ43714 antibody
    • G protein beta 5 subunit antibody
    • G protein beta subunit 5L antibody
    • GB 5 antibody
    • GB5 antibody
    • GBB5_HUMAN antibody
    • Gbeta5 antibody
    • GNB 5 antibody
    • GNB5 antibody
    • Guanine nucleotide binding protein (G protein) beta 5 antibody
    • Guanine nucleotide binding protein beta 5 antibody
    • Guanine nucleotide binding protein beta 5 subunit antibody
    • Guanine nucleotide binding protein beta subunit 5L antibody
    • Guanine nucleotide binding protein subunit beta 5 antibody
    • Guanine nucleotide-binding protein subunit beta-5 antibody
    • Transducin beta chain 5 antibody
    see all

画像

  • Western blot - Anti-Gbeta5 antibody [EPR14779(B)] (ab185214)
    Western blot - Anti-Gbeta5 antibody [EPR14779(B)] (ab185214)
    All lanes : Anti-Gbeta5 antibody [EPR14779(B)] (ab185214) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Gbeta5 antibody [EPR14779(B)] (ab185214)
    Western blot - Anti-Gbeta5 antibody [EPR14779(B)] (ab185214)
    Anti-Gbeta5 antibody [EPR14779(B)] (ab185214) at 1/1000 dilution + Human cerebellum tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?

  • Anti-Gbeta5 antibody [EPR14779(B)] (ab185214)
    Anti-Gbeta5 antibody [EPR14779(B)] (ab185214)

参考文献 (1)

ab185214 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab185214 は 1 報の論文で使用されています。

  • Shamseldin HE  et al. GNB5 mutation causes a novel neuropsychiatric disorder featuring attention deficit hyperactivity disorder, severely impaired language development and normal cognition. Genome Biol 17:195 (2016). WB ; Human . PubMed: 27677260

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