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リコンビナントRabMAb

Anti-GATA4 抗体 [EPR23782-19] - BSA and Azide free (Detector) (ab300783)

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Sandwich ELISA - Anti-GATA4 antibody [EPR23782-19] - BSA and Azide free (Detector) (ab300783)
  • Anti-GATA4 antibody [EPR23782-19] - BSA and Azide free (Detector) (AB300783)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23782-19] to GATA4 - BSA and Azide free (Detector)
  • Suitable for: Sandwich ELISA
  • Reacts with: Mouse, Human

製品の概要

  • 製品名

    Anti-GATA4 antibody [EPR23782-19] - BSA and Azide free (Detector)
    GATA4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23782-19] to GATA4 - BSA and Azide free (Detector)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    Ab300783 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify human, mouse GATA4. The recommended pair for sandwich ELISA is:
         Capture: ab300782, human, mouse GATA4 Capture Antibody (unconjugated)
         Detector: ab300783, human, mouse GATA4 Detector Antibody (unconjugated)
    The reference range value is 7.8 - 1000 pg/mL.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300783の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR23782-116] to GATA4 - BSA and Azide free (Capture) (ab300782).
特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR23782-116] to GATA4 - BSA and Azide free (Capture) (ab300782).

ターゲット情報

  • 機能

    Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).

  • 関連疾患

    Atrial septal defect 2
    Ventricular septal defect 1
    Tetralogy of Fallot
    Atrioventricular septal defect 4
    Testicular anomalies with or without congenital heart disease
    GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

  • 配列類似性

    Contains 2 GATA-type zinc fingers.

  • 翻訳後修飾

    Methylation at Lys-300 attenuates transcriptional activity.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ASD2 antibody
      • GATA 4 antibody
      • GATA binding protein 4 antibody
      • GATA-binding factor 4 antibody
      • gata4 antibody
      • GATA4_HUMAN antibody
      • MGC126629 antibody
      • Transcription factor GATA 4 antibody
      • Transcription factor GATA-4 antibody
      • Transcription factor GATA4 antibody
      • VSD1 antibody
      see all

    画像

    • Sandwich ELISA - Anti-GATA4 antibody [EPR23782-19] - BSA and Azide free (Detector) (ab300783)
      Sandwich ELISA - Anti-GATA4 antibody [EPR23782-19] - BSA and Azide free (Detector) (ab300783)
      Sandwich ELISA of AB300781 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
    • Anti-GATA4 antibody [EPR23782-19] - BSA and Azide free (Detector) (AB300783)
      Anti-GATA4 antibody [EPR23782-19] - BSA and Azide free (Detector) (AB300783)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300783 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300783 は論文での使用が確認できていません。

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