AB186371

Anti-GATA3 (phospho S308) 抗体 [EPR18118]

Name: Anti-GATA3 (phospho S308) 抗体 [EPR18118] (ab186371) | Abcam
Brand: Abcam Limited
SKU: AB186371
Price: 57000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Anti-GATA3 (phospho S308) antibody [EPR18118]

BOND RX™ Validated
RabMAb
Recombinant
詳細を見る

(1 Review)

(2 Publications)

Rabbit Recombinant Monoclonal GATA3 phospho S308 antibody. Suitable for IP, Dot, WB, IHC-P and reacts with Human, Mouse, Rat, Synthetic peptide - Human samples. Cited in 2 publications.

別名を表示する

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA3

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma tissue labeling GATA3 (phospho S308) with ab186371 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on tumor cells of Human transitional cell carcinoma of bladder is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling GATA3 (phospho S308) with ab186371 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on trophoblast cells of Human placenta is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

GATA3 (phospho S308) was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) cells treated with 1mM 8-Bromo-cAMP for 6 hours with ab186371 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab186371 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

Lane 1 : Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours, 10 μg (Input).

Lane 2 : ab186371 IP in Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab186371 in Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)

Predicted band size: 47 kDa

Observed band size: 48 kDa

false

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling GATA3 (phospho S308) with ab186371 at 1/50 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Nuclear staining on mouse stomach without Lambda Protein Phosphatase treatment (image A). No signal was detected when tissues were treated with Lambda Protein Phosphatase treatment (image B). The section was incubated with ab186371 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling GATA3 (phospho S308) with ab186371 at 1/50 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Nuclear staining on rat stomach without Lambda Protein Phosphatase treatment (image A). No signal was detected when tissues were treated with Lambda Protein Phosphatase treatment (image B). The section was incubated with ab186371 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Supplier Data

Western blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371) at 1/10000 dilution

Lane 1:

Untreated Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 10 µg

Lane 2:

Jurkat whole cell lysates treated with 1mM 8-Bromo-cAMP for 6 hours at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 47 kDa

Observed band size: 48 kDa

false

Exposure time: 15s

Lab

Dot Blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

Dot blot analysis of GATA3 (phospho S308) using ab186371 at 1 : 1000 (1.121 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody (ab97051) at 1 : 100000 dilution. Lane 1 : Human GATA3 (phospho Ser316) peptide Lane 2 : Human GATA3 (phospho Thr315) peptide Lane 3 : Human GATA3 (phospho Ser308) peptide Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds

Lab

Western blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371) at 1/1000 dilution

Lane 1:

Untreated EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

Lane 2:

EL4 treated with 0.5 mM 8-Bromo-cAMP for 6 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 48 kDa

false

Exposure time: 70s

Supplier Data

Dot Blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

Dot blot analysis of GATA3 (phospho S308) peptide (Lane 1) and non-phospho peptide (Lane 2) labeled using ab186371 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

Blocking/Dilution buffer : 5% NFDM/TBST..

Exposure time : 3 minutes.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR18118

アイソタイプ

IgG

キャリアフリー

交差種

Human, Mouse, Rat

アプリケーション

IP, Dot, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

The immunogen sequence for this antibody is identical to the sequence around S340 of GATA2 (UniProt P23769); however the phosphorylation of GATA2 on S340 has not been reported.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/70", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Synthetic peptide - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GATA3 also known as GATA-binding factor 3 is a transcription factor with a molecular mass of about 50 kDa. It belongs to the GATA family of zinc finger transcription factors. GATA3 is mainly expressed in the immune system especially in T-cells as well as in the nervous system and mammary glands. It plays a role in the regulation of critical developmental processes and cell lineage commitment through its binding to the 'GATA' motif in the promoter regions of target genes.

Biological function summary

GATA3 functions as a regulator of gene expression involved in various differentiation processes. It is not a solitary molecule; it interacts with other proteins to form transcriptional complexes. In T-helper type 2 (Th2) cells GATA3 is necessary for Th2 cell differentiation and cytokine production. Its expression is vital for the development of the nervous system and mammary gland morphogenesis highlighting its role in diverse biological contexts.

Pathways

GATA3 plays a significant role in the Th2 differentiation pathway and is linked to the immune response. It acts in association with other transcription factors like STAT6 and T-bet. GATA3 promotes the transcription of Th2 cytokines such as IL-4 IL-5 and IL-13 while suppressing Th1 responses. This balance orchestrated by GATA3 is important for immune homeostasis and the body's ability to respond to infections and allergens.

The dysregulation of GATA3 is associated with atopic diseases and breast cancer. Aberrant expression of GATA3 is often observed in allergic conditions due to its role in Th2 cell differentiation and cytokine expression. In the context of breast cancer GATA3 levels can impact tumor progression and are correlated with estrogen receptor status. Additionally its relation with other breast cancer-related proteins like L50 underlines its importance in cancer biology and potential as a therapeutic target.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression (By similarity). Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33. Upon tissue injury, acts downstream of IL33 signaling to drive differentiation of inflammation-resolving alternatively activated macrophages.

See full target information GATA3 phospho S308

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer research : BCR 22:139 PubMed33298139

2020

Cathepsin V suppresses GATA3 protein expression in luminal A breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Naphannop Sereesongsaeng,Sara H McDowell,James F Burrows,Christopher J Scott,Roberta E Burden

Molecular and cellular biology 35:11-25 PubMed25312646

2014

ATF1 modulates the heat shock response by regulating the stress-inducible heat shock factor 1 transcription complex.

Applications

Unspecified application

Species

Unspecified reactive species

Ryosuke Takii,Mitsuaki Fujimoto,Ke Tan,Eiichi Takaki,Naoki Hayashida,Ryuichiro Nakato,Katsuhiko Shirahige,Akira Nakai

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GATA3 (phospho S308) 抗体 [EPR18118]

Anti-GATA3 (phospho S308) antibody [EPR18118]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

Immunoprecipitation - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

Western blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

Dot Blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

Western blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

Dot Blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (AB186371)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

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