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リコンビナントRabMAb

Anti-GATA1 抗体 [EPR26488-109] (ab300613)

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Western blot - Anti-GATA1 antibody [EPR26488-109] (AB300613)
  • Western blot - Anti-GATA1 antibody [EPR26488-109] (AB300613)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR26488-109] (AB300613)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR26488-109] (AB300613)
  • Immunohistochemistry (Frozen sections) - Anti-GATA1 antibody [EPR26488-109] (AB300613)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26488-109] to GATA1
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-GATA1 antibody [EPR26488-109]
    GATA1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26488-109] to GATA1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IHC-Frmore details
    適用なし: Flow Cyt (Intra),ICC/IF or IP

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse spleen tissue lysate, rat spleen tissue lysate. IHC-P: Mouse spleen FFPE tissue section. IHCFr: Mouse spleen fresh frozen tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300613の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 43 kDa).
IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/100.
特記事項
WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 43 kDa).
IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/100.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF or IP.

ターゲット情報

  • 機能

    Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells.

  • 組織特異性

    Erythrocytes.

  • 関連疾患

    Defects in GATA1 are the cause of X-linked dyserythropoietic anemia and thrombocytopenia (XDAT) [MIM:300367]. XDAT is a disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes.
    Defects in GATA1 are the cause of X-linked thrombocytopenia with beta-thalassemia (XLTT) [MIM:314050]; also knwon as thrombocytopenia, platelet dysfunction, hemolysis, and imbalanced globin synthesis. XLTT consists of an unusual form of thrombocytopenia with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced hemoglobin chain synthesis resembling that of beta-thalassemia minor.
    Defects in GATA1 are the cause of anemia without thrombocytopenia X-linked (XLAWT) [MIM:300835]. XLAWT is a form of anemia characterized by abnormal morphology of erythrocytes and granulocytes in peripheral blood, bone marrow dysplasia with hypocellularity of erythroid and granulocytic lineages, and normal or increased number of megakaryocytes. Neutropenia of a variable degree is present in affected individuals.

  • 配列類似性

    Contains 2 GATA-type zinc fingers.

  • ドメイン

    The two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding.

  • 翻訳後修飾

    Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137.
    Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation by SUMO1 has no effect on transcriptional activity.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Anemia, X-linked, without thrombocytopenia, included antibody
      • ERYF 1 antibody
      • Eryf1 antibody
      • Erythroid transcription factor antibody
      • Erythrold transcription factor 1 antibody
      • GATA 1 antibody
      • GATA binding factor 1 antibody
      • GATA binding protein 1 (globin transcription factor 1) antibody
      • GATA binding protein 1 antibody
      • GATA-1 antibody
      • GATA-binding factor 1 antibody
      • GATA1 antibody
      • GATA1_HUMAN antibody
      • GF 1 antibody
      • GF-1 antibody
      • GF1 antibody
      • Globin transcription factor 1 antibody
      • NF E1 antibody
      • NF E1 DNA binding protein antibody
      • NF-E1 DNA-binding protein antibody
      • NFE 1 antibody
      • NFE1 antibody
      • Nuclear factor erythroid 1 antibody
      • Transcription factor GATA1 antibody
      • XLANP antibody
      • XLTDA antibody
      • XLTT antibody
      see all

    画像

    • Western blot - Anti-GATA1 antibody [EPR26488-109] (AB300613)
      Western blot - Anti-GATA1 antibody [EPR26488-109] (AB300613)
      All lanes : Anti-GATA1 antibody [EPR26488-109] (ab300613) at 1/1000 dilution

      Lane 1 : Mouse spleen tissue lysate
      Lane 2 : Mouse heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 43 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Negative control: heart (PMID: 33736593)

      Exposure time: 81 seconds

    • Western blot - Anti-GATA1 antibody [EPR26488-109] (AB300613)
      Western blot - Anti-GATA1 antibody [EPR26488-109] (AB300613)
      All lanes : Anti-GATA1 antibody [EPR26488-109] (ab300613) at 1/1000 dilution

      Lane 1 : Rat spleen tissue lysate
      Lane 2 : Rat heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 43 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Negative control: heart (PMID: 33736593)

      Exposure time: 70 seconds

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR26488-109] (AB300613)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR26488-109] (AB300613)

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GATA1 with ab300613 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse spleen (PMID:28240607). The section was incubated with ab300613 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR26488-109] (AB300613)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR26488-109] (AB300613)

      Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling GATA1 with ab300613 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining in mouse cardiac muscle. The section was incubated with ab300613 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Frozen sections) - Anti-GATA1 antibody [EPR26488-109] (AB300613)
      Immunohistochemistry (Frozen sections) - Anti-GATA1 antibody [EPR26488-109] (AB300613)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling GATA1 with ab300613 at 1/100 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Positive staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300613 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300613 は論文での使用が確認できていません。

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