Anti-GATA1 抗体 (ab11852)
Key features and details
- Rabbit polyclonal to GATA1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GATA1 antibody
GATA1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GATA1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
K-FPTGPMPPTTSTTVVAPLSS
, with N-terminal added Lysine, conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 394-413 of Human GATA1. -
ポジティブ･コントロール
- WB: Human erythroleukamia cells
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特記事項
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11852の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/400 - 1/800. Predicted molecular weight: 43 kDa.
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| 特記事項 |
|---|
|
WB
1/400 - 1/800. Predicted molecular weight: 43 kDa. |
ターゲット情報
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機能
Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. -
組織特異性
Erythrocytes. -
関連疾患
Defects in GATA1 are the cause of X-linked dyserythropoietic anemia and thrombocytopenia (XDAT) [MIM:300367]. XDAT is a disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes.
Defects in GATA1 are the cause of X-linked thrombocytopenia with beta-thalassemia (XLTT) [MIM:314050]; also knwon as thrombocytopenia, platelet dysfunction, hemolysis, and imbalanced globin synthesis. XLTT consists of an unusual form of thrombocytopenia with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced hemoglobin chain synthesis resembling that of beta-thalassemia minor.
Defects in GATA1 are the cause of anemia without thrombocytopenia X-linked (XLAWT) [MIM:300835]. XLAWT is a form of anemia characterized by abnormal morphology of erythrocytes and granulocytes in peripheral blood, bone marrow dysplasia with hypocellularity of erythroid and granulocytic lineages, and normal or increased number of megakaryocytes. Neutropenia of a variable degree is present in affected individuals. -
配列類似性
Contains 2 GATA-type zinc fingers. -
ドメイン
The two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding. -
翻訳後修飾
Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137.
Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation by SUMO1 has no effect on transcriptional activity. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2623 Human
- Omim: 305371 Human
- SwissProt: P15976 Human
- Unigene: 765 Human
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別名
- Anemia, X-linked, without thrombocytopenia, included antibody
- ERYF 1 antibody
- Eryf1 antibody
see all
画像
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Western blot - Anti-GATA1 antibody (ab11852)Lane 1 : Anti-GATA1 antibody (ab11852) at 1/400 dilution
Lane 2 : Anti-GATA1 antibody (ab11852) at 1/800 dilution
Lane 3 : Negative control
All lanes : K562 Human erythroleukamia cells
Predicted band size: 43 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (27)
ab11852 は 27 報の論文で使用されています。
- Kanezaki R et al. Mechanism of KIT gene regulation by GATA1 lacking the N-terminal domain in Down syndrome-related myeloid disorders. Sci Rep 12:20587 (2022). PubMed: 36447001
- Huang P et al. HIC2 controls developmental hemoglobin switching by repressing BCL11A transcription. Nat Genet 54:1417-1426 (2022). PubMed: 35941187
- Bozhilov YK et al. A gain-of-function single nucleotide variant creates a new promoter which acts as an orientation-dependent enhancer-blocker. Nat Commun 12:3806 (2021). PubMed: 34155213
- King AJ et al. Reactivation of a developmentally silenced embryonic globin gene. Nat Commun 12:4439 (2021). PubMed: 34290235
- Doerfler PA et al. Activation of γ-globin gene expression by GATA1 and NF-Y in hereditary persistence of fetal hemoglobin. Nat Genet 53:1177-1186 (2021). PubMed: 34341563