AB11852

Anti-GATA1 抗体

Anti-GATA1 antibody

5

(2 Reviews)

|

(32 Publications)

Anti-GATA1 antibody (ab11852) is a rabbit polyclonal antibody detecting GATA1 in Western Blot. Suitable for Human.

- Over 20 publications
- Trusted since 2004

別名を表示する

ERYF1, GF1, GATA1, Erythroid transcription factor, Eryf1, GATA-binding factor 1, NF-E1 DNA-binding protein, GATA-1, GF-1

1 Images

Western blot - Anti-GATA1 antibody (AB11852)

WB

Unknown

Western blot - Anti-GATA1 antibody (AB11852)

Lane 1:

Western blot - Anti-GATA1 antibody (ab11852) at 1/400 dilution

Lane 2:

Western blot - Anti-GATA1 antibody (ab11852) at 1/800 dilution

Lane 3:

Negative control

All lanes:

K562 Human erythroleukamia cells

Predicted band size: 43 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

免疫原

Synthetic Peptide within Human GATA1 aa 350-450 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB133274

Anti-GATA1 antibody [EPR2820(2)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-GATA1 antibody [EPR2820(2)] (AB133274)

0
0

Primary Antibodies

AB181544

Anti-GATA1 antibody [EPR17362] - ChIP Grade

20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR17362] - ChIP Grade (AB181544)

0
0

Primary Antibodies

AB76121

Anti-GATA1 antibody [EP2819Y]

RabMAb|Recombinant

Western blot - Anti-GATA1 antibody [EP2819Y] (AB76121)

0
0

Primary Antibodies

AB241393

Anti-GATA1 antibody [EPR17362] - ChIP Grade - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR17362] - ChIP Grade - BSA and Azide free (AB241393)

0
0

組合せ製品

Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

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Primary Antibodies

AB109411

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RabMAb|Recombinant|KO Validated|20ul selling size

4
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Primary Antibodies

AB191401

Anti-Ctip1/BCL-11A antibody [EPR14943-44]

Advanced Validation|RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

0
0

Primary Antibodies

AB32117

Anti-HDAC2 antibody [Y461]

20ul selling size|BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC2 antibody [Y461] (AB32117)

5
12

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/400 - 1/800", "WB-species-notes": "<p></p>" } } }

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製品の詳細

What is this antibody validated in?
Anti-GATA1 antibody (ab11852) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of GATA1?
Anti-GATA1 (ab11852) specifically detects a band for GATA1 (UniProt: P15976) at a molecular weight of 43kDa.

Trusted by the scientific community
Anti-GATA1 (ab11852) was first used in a scientific publication in 2004 and has been cited over 20 times in peer-reviewed journals.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GATA1 also known as GATA-binding factor 1 is a transcription factor weighing approximately 42 kDa. This protein binds to the DNA sequence "GATA" within the regulatory regions of target genes to control their expression. GATA1 is mostly expressed in hematopoietic cells especially erythroid cells megakaryocytes eosinophils and mast cells. Its specific expression pattern allows it to regulate genes essential for the development and function of these cell types.

Biological function summary

GATA1 regulates gene expression and is essential for erythroid and megakaryocyte differentiation. GATA1 belongs to a family of GATA transcription factors and can form regulatory complexes with other proteins such as FOG1 (Friend of GATA1) and TAL1. These complexes facilitate the binding of GATA1 to DNA enhancing its transcriptional activity on target gene sets necessary for blood cell maturation and function.

Pathways

GATA1 participates in the erythropoiesis and megakaryopoiesis pathways. It directly regulates several genes and coordinates signals with factors like GATA2 and RUNX1 which are other key proteins involved in these pathways. Through these interactions GATA1 ensures the correct development of red blood cells and platelets maintaining hematopoietic homeostasis in the organism.

Abnormal GATA1 function is linked to blood-related disorders. Mutations or dysregulations of GATA1 can lead to diseases such as X-linked thrombocytopenia with dyserythropoietic anemia which involves low platelet count and ineffective red blood cell production. Moreover GATA1 mutations are associated with certain leukemias particularly acute megakaryoblastic leukemia. GATA1's dysfunction in these diseases often occurs in conjunction with abnormalities in related proteins like GATA2 influencing disease progression and phenotype.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcriptional activator or repressor which serves as a general switch factor for erythroid development (PubMed : 35030251). It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed : 24245781).

See full target information GATA1

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文献 (32)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed40424086

2025

Splicing of erythroid transcription factor is associated with therapeutic response in myelodysplastic syndromes.

Applications

Unspecified application

Species

Unspecified reactive species

Srinivas Aluri,Te Ling,Ellen Fraint,Samarpana Chakraborty,Kevin Zhang,Aarif Ahsan,Leah Kravets,Gowri Poigaialwar,Rongbao Zhao,Kith Pradhan,Anitria Cotton,Kimo Bachiashvili,Jung-In Yang,Anjali Budhathoki,Beamon Agarwal,Shanisha Gordon Mitchell,Milagros Carbajal,Srabani Sahu,Jacqueline Boultwood,Andrea Pellagatti,Ulrich Steidl,Amittha Wickrema,Satish Nandakumar,Aditi Shastri,Rajasekhar Nvs Suragani,Teresa V Bowman,John D Crispino,Sadanand Vodala,Amit Verma

Journal of sickle cell disease 1:yoae008 PubMed40304012

2025

Hydroxyurea reduces the levels of the fetal gene repressors ZBTB7A/LRF and BCL11A in erythroid cells .

Applications

Unspecified application

Species

Unspecified reactive species

Gabriella E Martyn,Phillip A Doerfler,Yu Yao,Kate G R Quinlan,Mitchell J Weiss,Merlin Crossley

Nucleic acids research 51:6634-6653 PubMed37254808

2023

Multidimensional profiling reveals GATA1-modulated stage-specific chromatin states and functional associations during human erythropoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Li,Xin-Ying Zhao,Shuo Zhou,Qi Hu,Fan Wu,Hsiang-Ying Lee

Scientific reports 12:20587 PubMed36447001

2022

Mechanism of KIT gene regulation by GATA1 lacking the N-terminal domain in Down syndrome-related myeloid disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Rika Kanezaki,Tsutomu Toki,Kiminori Terui,Tomohiko Sato,Akie Kobayashi,Ko Kudo,Takuya Kamio,Shinya Sasaki,Koji Kawaguchi,Kenichiro Watanabe,Etsuro Ito

Nature genetics 54:1417-1426 PubMed35941187

2022

HIC2 controls developmental hemoglobin switching by repressing BCL11A transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Huang,Scott A Peslak,Ren Ren,Eugene Khandros,Kunhua Qin,Cheryl A Keller,Belinda Giardine,Henry W Bell,Xianjiang Lan,Malini Sharma,John R Horton,Osheiza Abdulmalik,Stella T Chou,Junwei Shi,Merlin Crossley,Ross C Hardison,Xiaodong Cheng,Gerd A Blobel

Nature genetics 53:1177-1186 PubMed34341563

2021

Activation of γ-globin gene expression by GATA1 and NF-Y in hereditary persistence of fetal hemoglobin.

Applications

Unspecified application

Species

Unspecified reactive species

Phillip A Doerfler,Ruopeng Feng,Yichao Li,Lance E Palmer,Shaina N Porter,Henry W Bell,Merlin Crossley,Shondra M Pruett-Miller,Yong Cheng,Mitchell J Weiss

Nature communications 12:4439 PubMed34290235

2021

Reactivation of a developmentally silenced embryonic globin gene.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew J King,Duantida Songdej,Damien J Downes,Robert A Beagrie,Siyu Liu,Megan Buckley,Peng Hua,Maria C Suciu,A Marieke Oudelaar,Lars L P Hanssen,Danuta Jeziorska,Nigel Roberts,Stephanie J Carpenter,Helena Francis,Jelena Telenius,Aude-Anais Olijnik,Jacqueline A Sharpe,Jacqueline Sloane-Stanley,Jennifer Eglinton,Mira T Kassouf,Stuart H Orkin,Len A Pennacchio,James O J Davies,Jim R Hughes,Douglas R Higgs,Christian Babbs

Nature communications 12:3806 PubMed34155213

2021

A gain-of-function single nucleotide variant creates a new promoter which acts as an orientation-dependent enhancer-blocker.

Applications

Unspecified application

Species

Unspecified reactive species

Yavor K Bozhilov,Damien J Downes,Jelena Telenius,A Marieke Oudelaar,Emmanuel N Olivier,Joanne C Mountford,Jim R Hughes,Richard J Gibbons,Douglas R Higgs

Cell death & disease 12:223 PubMed33637692

2021

Regulation of MYB by distal enhancer elements in human myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjia Li,Penglei Jiang,Kai Cheng,Zehui Zhang,Shuyu Lan,Xiaoxia Li,Lirong Zhao,Yucheng Wang,Xiang Wang,Jing Chen,Tao Ji,Bingshe Han,Junfang Zhang

Blood 137:1327-1339 PubMed33512425

2021

Dynamic CTCF binding directly mediates interactions among cis-regulatory elements essential for hematopoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Qi,Li Cheng,Xing Tang,Yanghua He,Yichao Li,Tiffany Yee,Dewan Shrestha,Ruopeng Feng,Peng Xu,Xin Zhou,Shondra Pruett-Miller,Ross C Hardison,Mitchell J Weiss,Yong Cheng

View all publications

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