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Anti-GAPDH 抗体 [mAbcam 9484] - BSA and Azide free (ab174646)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [mAbcam 9484] - BSA and Azide free (ab174646)

    Key features and details

    • Mouse monoclonal [mAbcam 9484] to GAPDH - BSA and Azide free
    • Suitable for: IHC-P, WB
    • Reacts with: Mouse, Rat, Chicken, Cow, Human, Pig, Xenopus laevis, Chinese hamster
    • Isotype: IgG2b

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 790 HRP

    製品の概要

    • 製品名

      Anti-GAPDH antibody [mAbcam 9484] - BSA and Azide free
      GAPDH 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [mAbcam 9484] to GAPDH - BSA and Azide free

    • 由来種

      Mouse

    • アプリケーション

      適用あり: IHC-P, WBmore details

    • 種交差性

      交差種: Mouse, Rat, Chicken, Cow, Human, Pig, Xenopus laevis, Chinese hamster
      交差が予測される動物種: Rabbit, Dog, Cynomolgus monkey

    • 免疫原

      Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

    • 特記事項

      ab174646 is the carrier-free version of ab9484.

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab174646の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    Use a concentration of 5 µg/ml.
    WB
    Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 40.2 kDa.

    NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this antibody following concerning customer feedback on the lack of signal with some of the vials. We found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see images). This change in the characteristics of the antibody is due to a recent update to the production process.
    特記事項
    IHC-P
    Use a concentration of 5 µg/ml.
    WB
    Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 40.2 kDa.

    NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this antibody following concerning customer feedback on the lack of signal with some of the vials. We found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see images). This change in the characteristics of the antibody is due to a recent update to the production process.

    ターゲット情報

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [mAbcam 9484] - BSA and Azide free (ab174646)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [mAbcam 9484] - BSA and Azide free (ab174646)

      This IHC data was generated using the same antibody clone in a different buffer formulation (ab9484). 

      IHC image of GAPDH staining in human liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9484, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    参考文献 (0)

    ab174646 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab174646 は論文での使用が確認できていません。

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