Anti-GAPDH 抗体 - Loading Control

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB37168)

ICC/IF image of ab37168 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37168, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB37168)

ICC/IF image of ab37168 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37168, 1μg/ml) overnight at +4°C. The secondary antibody (green) was anti-Rabbit DyLight® 488 pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (AB37168)

ab37168 at 10μg/ml staining GAPDH in human liver tissue by IHC.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (AB37168)

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GAPDH antibody (ab37168) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as secondary. Counter stained with Hematoxylin.

• WB

Supplier Data

Western blot - Anti-GAPDH antibody - Loading Control (AB37168)

1 h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-GAPDH antibody - Loading Control (ab37168) at 0.5 µg/mL

Lane 1:

293 cell lysates at 15 µg

Lane 2:

A549 cell lysates at 15 µg

Lane 3:

HeLa cell lysates at 15 µg

Lane 4:

HepG2 cell lysates at 15 µg

Lane 5:

K562 cell lysates at 15 µg

Lane 6:

MCF7 cell lysates at 15 µg

Lane 7:

SK-N-SH cell lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

• WB

Supplier Data

Western blot - Anti-GAPDH antibody - Loading Control (AB37168)

1 h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-GAPDH antibody - Loading Control (ab37168) at 1 µg/mL

Lane 1:

Rat skin at 15 µg

Lane 2:

Rat brain at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

• WB

Supplier Data

Western blot - Anti-GAPDH antibody - Loading Control (AB37168)

1 h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-GAPDH antibody - Loading Control (ab37168) at 1 µg/mL

Lane 1:

Mouse brain at 15 µg

Lane 2:

Mouse thymus at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody - Loading Control (AB37168)

All lanes:

Western blot - Anti-GAPDH antibody - Loading Control (ab37168) at 1 µg/mL

Lanes 1 and 3:

HeLa Whole Cell Lysate at 10 µg

Lanes 2 and 4:

Jurkat Whole Cell Lysate at 10 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.02 µg/mL

Lanes 3 - 4:

Western blot - VHH Single Domain Anti-Rabbit IgG Fc (HRP) (<a href='/products/secondary-antibodies/vhh-single-domain-rabbit-igg-fc-hrp-ab191866'>ab191866</a>) at 0.02 µg/mL

Predicted band size: 11 kDa,54 kDa

true