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Anti-GAPDH 抗体 - Loading Control (ab22555)

Reviews (4)Q&A (1)References (106)
Western blot - Anti-GAPDH antibody - Loading Control (ab22555)
  • Western blot - Anti-GAPDH antibody - Loading Control (ab22555)

Key features and details

  • Rabbit polyclonal to GAPDH - Loading Control
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Dog, Human, African green monkey
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-GAPDH antibody - Loading Control
    GAPDH 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to GAPDH - Loading Control

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Dog, Human, African green monkey
    交差が予測される動物種: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Turkey, Pig, Duck

  • 免疫原

    Synthetic peptide:

    DAPMFVMGVNHFKYA

    , corresponding to amino acids 126-140 of Human GAPDH.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab22555の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (4)
1/1000 - 1/5000. Detects a band of approximately 36 kDa.
特記事項
WB
1/1000 - 1/5000. Detects a band of approximately 36 kDa.

ターゲット情報

画像

  • Western blot - Anti-GAPDH antibody - Loading Control (ab22555)
    Western blot - Anti-GAPDH antibody - Loading Control (ab22555)
    Lane 1 : Anti-GAPDH antibody - Loading Control (ab22555)
    Lanes 2-4 : Anti-GAPDH antibody - Loading Control (ab22555) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HUVEC cell lysate
    Lane 4 : PC12 cell lysate

    Lysates/proteins at 25 µg per lane.
  • Western blot - Anti-GAPDH antibody - Loading Control (ab22555)
    Western blot - Anti-GAPDH antibody - Loading Control (ab22555)
    All lanes : Anti-GAPDH antibody - Loading Control (ab22555) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : COS-7 cell lysate
    Lane 3 : MDCK cell lysate
    Lane 4 : PC-3 cell lysate
    Lane 5 : Mouse brain tissue lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ HRP conjugate at 1/4000 dilution

    Observed band size: 37 kDa why is the actual band size different from the predicted?

参考文献 (106)

ab22555 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab22555 は 106 報の論文で使用されています。

  • Pinheiro-de-Sousa I  et al. Uncovering emergent phenotypes in endothelial cells by clustering of surrogates of cardiovascular risk factors. Sci Rep 12:1372 (2022). PubMed: 35079076
  • Zhang Z  et al. Icariin regulates stem cell migration for endogenous repair of intervertebral disc degeneration by increasing the expression of chemotactic cytokines. BMC Complement Med Ther 22:63 (2022). PubMed: 35272637
  • Sathiyanadan K  et al. Targeting Endothelial Connexin37 Reduces Angiogenesis and Decreases Tumor Growth. Int J Mol Sci 23:N/A (2022). PubMed: 35328350
  • Ma X  et al. TRAF2, an Innate Immune Sensor, Reciprocally Regulates Mitophagy and Inflammation to Maintain Cardiac Myocyte Homeostasis. JACC Basic Transl Sci 7:223-243 (2022). PubMed: 35411325
  • Xu F  et al. SLC1A5 Prefers to Play as an Accomplice Rather Than an Opponent in Pancreatic Adenocarcinoma. Front Cell Dev Biol 10:800925 (2022). PubMed: 35419359
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-GAPDH antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (4-17%)
Loading amount
10 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 16 2018

Western blot abreview for Anti-GAPDH antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10%SDS gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 30 2010

Western blot abreview for Anti-GAPDH antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 15 2010

Western blot abreview for Anti-GAPDH antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Baboon Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 08 2010

Question

Customer kindly called to find replacement product for ab36840 in use on planaria.

Read More
Answer

Thank you very much for your interest in anti-GAPDH antibodies.

I have reviewed the products in our catalog in effort to try an find for you a product which was most similar to ab36840. I believe that the following two products offer the best alternative.

AB75834 (https://www.abcam.com/gapdh-antibody-epr1977y-ab75834.html). This rabbit monoclonal was raised against a very similar region to ab36840. Therefore it may be likely that the same epitope site will be recogized by this product.

AB22555 (https://www.abcam.com/gapdh-antibody-ab22555.html). This rabbit polyclonal was raised against an area just before that which ab36840 was. While this product will not be recognizing the same epitopes it's polyclonal nature will mean that it will recognize more sites than a monoclonal antibody.

To our knowledge, these have not been tested in planaria. Therefore, I can offer a discount off a future purchase if you buy an GAPDH antibody now, test it in planaria and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test one of these in planaria. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

2. Purchase either by phone, fax, or online (www.abcam.com).

3. Test it in planaria.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the product turns out to be unsuitable for planaria, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com