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AB22555

Anti-GAPDH 抗体 - Loading Control

Anti-GAPDH antibody - Loading Control

5

(4 Reviews)

|

(114 Publications)

Rabbit Polyclonal GAPDH antibody. Suitable for WB and reacts with Mouse, Rat, African green monkey, Human, Dog samples. Cited in 114 publications. Immunogen corresponding to Synthetic Peptide within Human GAPDH aa 100-150.

別名を表示する

GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

2 Images
Western blot - Anti-GAPDH antibody - Loading Control (AB22555)
  • WB

Supplier Data

Western blot - Anti-GAPDH antibody - Loading Control (AB22555)

Lane 1:

Western blot - Anti-GAPDH antibody - Loading Control (ab22555)

Lanes 2 - 4:

Western blot - Anti-GAPDH antibody - Loading Control (ab22555) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 25 µg

Lane 2:

293 cell lysate at 25 µg

Lane 3:

HUVEC cell lysate at 25 µg

Lane 4:

PC12 cell lysate at 25 µg

Predicted band size: 36 kDa

false

Western blot - Anti-GAPDH antibody - Loading Control (AB22555)
  • WB

Supplier Data

Western blot - Anti-GAPDH antibody - Loading Control (AB22555)

All lanes:

Western blot - Anti-GAPDH antibody - Loading Control (ab22555) at 1/1000 dilution

Lane 1:

A431 cell lysate at 30 µg

Lane 2:

COS-7 cell lysate at 30 µg

Lane 3:

MDCK cell lysate at 30 µg

Lane 4:

PC-3 cell lysate at 30 µg

Lane 5:

Mouse brain tissue lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ HRP conjugate at 1/4000 dilution

Predicted band size: 36 kDa

Observed band size: 37 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Dog, Human, African green monkey

アプリケーション

WB

applications

免疫原

Synthetic Peptide within Human GAPDH aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P04406

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Duck": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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下記製品もご検討ください

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

5
23 Reviews
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私たちは、より高品質な製品をお届けするために、製品構成を常に改善しています。今回ご紹介する代替製品は、性能の向上、使いやすさや利便性の向上、またはより早くお届けできる点などを踏まえて選定しています。

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.
Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed : 11724794, PubMed : 3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed : 11724794, PubMed : 3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed : 23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed : 23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed : 23332158, PubMed : 27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
See full target information GAPDH
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文献 (114)

Recent publications for all applications. Explore the full list and refine your search

Molecular systems biology 19:e11462 PubMed38031960

2023

Integrated systems biology approach identifies gene targets for endothelial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Iguaracy Pinheiro-de-Sousa,Miriam Helena Fonseca-Alaniz,Girolamo Giudice,Iuri Cordeiro Valadão,Silvestre Massimo Modestia,Sarah Viana Mattioli,Ricardo Rosa Junior,Lykourgos-Panagiotis Zalmas,Yun Fang,Evangelia Petsalaki,José Eduardo Krieger

Nature cell biology 25:1804-1820 PubMed38012402

2023

COPI vesicle formation and N-myristoylation are targetable vulnerabilities of senescent cells.

Applications

Unspecified application

Species

Unspecified reactive species

Domhnall McHugh,Bin Sun,Carmen Gutierrez-Muñoz,Fernanda Hernández-González,Massimiliano Mellone,Romain Guiho,Imanol Duran,Joaquim Pombo,Federico Pietrocola,Jodie Birch,Wouter W Kallemeijn,Sanjay Khadayate,Gopuraja Dharmalingam,Santiago Vernia,Edward W Tate,Juan Pedro Martínez-Barbera,Dominic J Withers,Gareth J Thomas,Manuel Serrano,Jesús Gil

Genes and environment : the official journal of the Japanese Environmental Mutagen Society 45:24 PubMed37817266

2023

LncRNA PVT1 induces apoptosis and inflammatory response of bronchial epithelial cells by regulating miR-30b-5p/BCL2L11 axis in COPD.

Applications

Unspecified application

Species

Unspecified reactive species

Taoli Fu,Hui Tian,Hui Rong,Ping Ai,Xiaoping Li

Biology of sex differences 13:66 PubMed36348414

2022

Sex differences in the medial prefrontal cortical glutamate system.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa C Knouse,Anna G McGrath,Andre U Deutschmann,Matthew T Rich,Lia J Zallar,Anjali M Rajadhyaksha,Lisa A Briand

JACC. Basic to translational science 7:1214-1228 PubMed36644282

2022

Targeting the Autophagy-Lysosome Pathway in a Pathophysiologically Relevant Murine Model of Reversible Heart Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Evans,Xiucui Ma,Xiqiang Wang,Yana Chen,Chen Zhao,Carla J Weinheimer,Attila Kovacs,Brian Finck,Abhinav Diwan,Douglas L Mann

Pharmaceutics 14: PubMed36297479

2022

NIL10: A New IL10-Receptor Binding Nanoparticle That Induces Cardiac Protection in Mice and Pigs Subjected to Acute Myocardial Infarction through STAT3/NF-κB Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Tesoro,Ignacio Hernández,Rafael Ramírez-Carracedo,Javier Díez-Mata,Nunzio Alcharani,Beatriz Jiménez-Guirado,Karina Ovejero-Paredes,Marco Filice,Jose Luis Zamorano,Marta Saura,Carlos Zaragoza,Laura Botana

Frontiers in immunology 13:995974 PubMed36203606

2022

Identification of immune-related endoplasmic reticulum stress genes in sepsis using bioinformatics and machine learning.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Gong,Yongbin Liu,Zhiyuan Tian,Min Zhang,Hejun Gao,Zhiyong Peng,Shuang Yin,Chi Wai Cheung,Youtan Liu

The Journal of biological chemistry 298:102485 PubMed36108742

2022

The SET-domain protein CgSet4 negatively regulates antifungal drug resistance via the ergosterol biosynthesis transcriptional regulator CgUpc2a.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanka Bhakt,Mayur Raney,Rupinder Kaur

Frontiers in surgery 9:893977 PubMed35784919

2022

N7-Methylguanosine Regulatory Genes Profoundly Affect the Prognosis, Progression, and Antitumor Immune Response of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kexiang Zhou,Jiaqun Yang,Xiaoyan Li,Wei Xiong,Pengbin Zhang,Xuqing Zhang

JACC. Basic to translational science 7:223-243 PubMed35411325

2022

TRAF2, an Innate Immune Sensor, Reciprocally Regulates Mitophagy and Inflammation to Maintain Cardiac Myocyte Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiucui Ma,David R Rawnsley,Attila Kovacs,Moydul Islam,John T Murphy,Chen Zhao,Minu Kumari,Layla Foroughi,Haiyan Liu,Kevin Qi,Aaradhya Diwan,Krzysztof Hyrc,Sarah Evans,Takashi Satoh,Brent A French,Kenneth B Margulies,Ali Javaheri,Babak Razani,Douglas L Mann,Kartik Mani,Abhinav Diwan
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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