Anti-GAPDH 抗体 - Loading Control (ab157156)
Key features and details
- Goat polyclonal to GAPDH - Loading Control
- Suitable for: IHC-Fr, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GAPDH antibody - Loading Control
GAPDH 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to GAPDH - Loading Control -
由来種
Goat -
アプリケーション
適用あり: IHC-Fr, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish, Orangutan, Xenopus tropicalis
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免疫原
Synthetic peptide within Human GAPDH aa 175-225. The exact sequence is proprietary.
Database link: P04406 -
ポジティブ･コントロール
- 293T, HeLa, Jurkat and mouse NIH 3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab157156 was affinity purified using an epitope specific to GAPDH immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab157156の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
1/500 - 1/2000.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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| WB | (2) |
1/500 - 1/2500. Predicted molecular weight: 36 kDa.
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| 特記事項 |
|---|
|
IHC-Fr
1/500 - 1/2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
|
WB
1/500 - 1/2500. Predicted molecular weight: 36 kDa. |
ターゲット情報
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機能
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
パスウェイ
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
配列類似性
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
翻訳後修飾
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
細胞内局在
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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参照データベース
- Entrez Gene: 374193 Chicken
- Entrez Gene: 281181 Cow
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 100172694 Orangutan
- Entrez Gene: 396823 Pig
- Entrez Gene: 100009074 Rabbit
see all -
別名
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
画像
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Immunohistochemistry (Frozen sections) - Anti-GAPDH antibody - Loading Control (ab157156)
Immunohistochemical analysis of frozen sections of human colon carcinoma tissue labelling GAPDH with ab157156 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-GAPDH antibody - Loading Control (ab157156)All lanes : Anti-GAPDH antibody - Loading Control (ab157156) at 1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 36 kDa
Exposure time: 30 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab157156 は 8 報の論文で使用されています。
- Zhang L et al. Investigation of the Association between the Energy Metabolism of the Insect Vector Laodelphax striatellus and Rice Stripe Virus (RSV). Viruses 14:N/A (2022). PubMed: 36298853
- Victor AK et al. Molecular Changes in Prader-Willi Syndrome Neurons Reveals Clues About Increased Autism Susceptibility. Front Mol Neurosci 14:747855 (2021). PubMed: 34776864
- Eddy AC et al. Differential regulation of sFlt-1 splicing by U2AF65 and JMJD6 in placental-derived and endothelial cells. Biosci Rep 40:N/A (2020). PubMed: 32039444
- Li Y et al. The a-tubulin of Laodelphax striatellus mediates the passage of rice stripe virus (RSV) and enhances horizontal transmission. PLoS Pathog 16:e1008710 (2020). PubMed: 32817722
- Hope KA et al. Transcriptomic and proteomic profiling of glial versus neuronal Dube3a overexpression reveals common molecular changes in gliopathic epilepsies. Neurobiol Dis 141:104879 (2020). PubMed: 32344153
- Li Y et al. YY1-induced upregulation of FOXP4-AS1 and FOXP4 promote the proliferation of esophageal squamous cell carcinoma cells. Cell Biol Int 44:1447-1457 (2020). PubMed: 32159250
- Urraca N et al. Significant transcriptional changes in 15q duplication but not Angelman syndrome deletion stem cell-derived neurons. Mol Autism 9:6 (2018). WB . PubMed: 29423132
- Jin S et al. MiR-873 inhibition enhances gefitinib resistance in non-small cell lung cancer cells by targeting glioma-associated oncogene homolog 1. Thorac Cancer 9:1262-1270 (2018). PubMed: 30126075