Anti-GAPDH 抗体 [6C5] - Loading Control

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

ab8245 staining GAPDH in SV40LT-SMC (Rat SV40-transfected aorta smooth cell line) cells.

The cells were fixed with 4% formaldehyde (10 minutes) permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1 hour. The cells were then incubated with ab8245 at 5μg/ml and ab202272 at 1/250 dilution overnight at +4°C followed by a further incubation at room temperature for 1h with Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150117) (shown in green). Nuclear DNA was labeled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

ab8245 staining GAPDH in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

The cells were fixed with 100% methanol (5 minutes) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1 hour. The cells were then incubated with ab8245 at 5 μg/ml and ab6046 at 1 μg/ml overnight at +4°C followed by a further incubation at room temperature for 1 hour with Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150117) at 2 μg/ml (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) preadsorbed (ab150088) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labeled in blue with DAPI.

Negative controls : 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

ab8245 staining GAPDH in NIH/3T3 (Mouse embryo fibroblast cell line) cells.

The cells were fixed with 4% formaldehyde (10 minutes) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1 hour. The cells were then incubated with ab8245 at 1 μg/ml and ab202272 at 1/250 dilution overnight at +4°C followed by a further incubation at room temperature for 1 hour with Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150117) (shown in green). Nuclear DNA was labeled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-Transferrin Receptor antibody [EPR20584] ab214039 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 90 kDa in Wild-type K562 cell line with a truncated product observed at 80 kDa in TFRC CRISPR-Cas9 edited K562 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Transferrin Receptor antibody [EPR20584] (<a href='/products/primary-antibodies/transferrin-receptor-antibody-epr20584-ab214039'>ab214039</a>) at 1/1000 dilution

Lane 1:

Wild-type K562 cell lysates at 20 µg

Lane 2:

Western blot - Human TFRC knockout K562 cell line (ab300266) at 20 µg

Lane 3:

HDLM-2 cell lysates at 20 µg

Lane 4:

SH-SY5Y cell lysates at 20 µg

Lane 5:

A549 cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 90 kDa

Observed band size: 90 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-DLG4 antibody [EPR23124-118] (ab238135) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab238135 was shown to bind specifically to DLG4. A band was observed at 80 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in DLG4 knockout cell line. To generate this image, wild-type and DLG4 knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (<a href='/products/primary-antibodies/psd95-antibody-epr23124-118-synaptic-marker-ab238135'>ab238135</a>) at 1/2000 dilution

Lane 1:

Wild-type U-87 MG cell lysate at 20 µg

Lane 2:

DLG4 knockout U-87 MG cell lysate at 20 µg

Lane 3:

Wild-type SH-SY5Y cell lysate at 20 µg

Lane 4:

DLG4 knockout SH-SY5Y <a href='/products/cell-lines/human-psd95-knockout-sh-sy5y-cell-line-ab280043'>ab280043</a> cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-MFN2 antibody [NIAR164] (ab124773) staining at 1/5000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab124773 was shown to bind specifically to MFN2. A band was observed at 64 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in MFN2 knockout cell line. To generate this image, wild-type and MFN2 knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Mitofusin 2 antibody [NIAR164] (<a href='/products/primary-antibodies/mitofusin-2-antibody-niar164-ab124773'>ab124773</a>) at 1/5000 dilution

Lane 1:

Wild-type HEK-293 cell lysate at 20 µg

Lane 2:

MFN2 knockout HEK-293 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

PC-3 cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 64 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-COIL antibody (ab210785) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab210785 was shown to bind specifically to COIL. A band was observed at 68 kDa in wild-type HeLa cell lysates with no signal observed at this size in COIL knockout cell line. To generate this image, wild-type and COIL knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Coilin antibody (<a href='/products/primary-antibodies/coilin-antibody-ab210785'>ab210785</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

COIL knockout HeLa cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 68 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Anti-LATS1 antibody [C66B5] staining at 1/1000 dilution, shown in black; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab3477S was shown to bind specifically to LATS1. A band was observed at 100-150 kDa in wild-type THP-1 cell lysates with no signal observed at this size in LATS1 knockout cell line. To generate this image, wild-type and LATS1 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % BSA in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 20 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Anti-LATS1 antibody at 1/1000 dilution

Lane 1:

Wild-type THP-1 cell lysate at 20 µg

Lane 2:

LATS1 knockout THP-1 cell lysate at 20 µg

Lane 3:

HT-29 cell lysate at 20 µg

Lane 4:

PC-3 cell lysate at 20 µg

Lane 5:

HeLa cell lysate at 20 µg

Lane 6:

NIH/3T3 cell lysate at 20 µg

Predicted band size: 126 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-FACL4 antibody [EPR8640] ab155282 staining at 1/10000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 79 kDa in Wild-type A431 cell line with no signal observed at this size in ACSL4 knockout A431 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-FACL4 antibody [EPR8640] (<a href='/products/primary-antibodies/facl4-antibody-epr8640-ab155282'>ab155282</a>) at 1/10000 dilution

Lane 1:

Wild-type A431 cell lysates at 20 µg

Lane 2:

Western blot - Human ACSL4 (FACL4) knockout A-431 cell line (<a href='/products/cell-lines/human-acsl4-facl4-knockout-a-431-cell-line-ab269613'>ab269613</a>) at 20 µg

Lane 3:

HEK-293 cell lysates at 20 µg

Lane 4:

K562 cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 79 kDa

Observed band size: 79 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Anti-RARA antibody [EPR23871-271] (ab275745) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab275745 was shown to bind specifically to RARA. A band was observed at 50-55 kDa in wild-type MCF7 cell lysates with no signal observed at this size in RARA knockout cell line ab277158 (knockout cell lysate ab281383). To generate this image, wild-type and RARA knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-271] (<a href='/products/primary-antibodies/retinoic-acid-receptor-alpha-antibody-epr23871-271-ab275745'>ab275745</a>) at 1/1000 dilution

Lane 1:

Wild-type MCF7 cell lysate at 20 µg

Lane 2:

RARA knockout MCF7 cell lysate at 20 µg

Lane 3:

Human Testis cell lysate at 20 µg

Lane 4:

Human Brain cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 51 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Anti-EZH1 antibody [D7D5D] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab42088S was shown to bind specifically to EZH1. A band was observed at 95 kDa in wild-type THP-1 cell lysates with no signal observed at this size in EZH1 knockout cell line ab280072 (knockout cell lysate ab280131). To generate this image, wild-type and EZH1 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Anti-EZH1 antibody at 1/1000 dilution

Lane 1:

Wild-type THP-1 cell lysate at 20 µg

Lane 2:

EZH1 knockout THP-1 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 95 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

False colour image of Western blot : Anti-PINK1 antibody staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to PINK1. A band was observed at 63 kDa in wild-type cell lysates with no signal observed at this size in PINK1 knockout cell line ab266393 (knockout cell lysate ab257030). To generate this image, wild-type and PINK1 knockout cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Anti-PINK1 antibody at 1/1000 dilution

Lane 1:

Wild-type HEK-293T Vehicle Control CCCP (0 μM, 24 h) cell lysate at 20 µg

Lane 2:

Wild-type HEK-293T Treated CCCP (10 μM, 24 h) cell lysate at 20 µg

Lane 3:

PINK1 knockout HEK-293T Vehicle Control CCCP (0 μM, 24 h) cell lysate at 20 µg

Lane 4:

PINK1 knockout HEK-293T Treated CCCP (10 μM, 24 h) cell lysate at 20 µg

Lane 5:

HeLa cell lysate at 20 µg

Lane 6:

Daudi cell lysate at 20 µg

Secondary

Lanes 1 - 6:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Lanes 1 - 6:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Predicted band size: 63 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-CD19 antibody [RM2072] ab322345 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 80-90 kDa in Wild-type Raji cell lysates with no signal observed at this size in CD19 knockout Raji cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CD19 antibody [RM2072] (<a href='/products/primary-antibodies/cd19-antibody-rm2072-ab322345'>ab322345</a>) at 1/1000 dilution

Lane 1:

Wild-type Raji at 20 µg

Lane 2:

Western blot - Human CD19 knockout Raji cell line (<a href='/products/cell-lines/human-cd19-knockout-raji-cell-line-ab274913'>ab274913</a>) at 20 µg

Lane 3:

Ramos at 20 µg

Lane 4:

MCF7 at 20 µg

Lane 5:

Daudi at 20 µg

Lane 6:

Jurkat at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 80-90 kDa

Observed band size: 80-90 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-Cytokeratin 18 antibody [EPR1625] ab133272 staining at 1/10000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 48 kDa in Wild-type A549 cell line with no signal observed at this size in KRT18 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Cytokeratin 18 antibody [EPR1625] (<a href='/products/primary-antibodies/cytokeratin-18-antibody-epr1625-ab133272'>ab133272</a>) at 1/10000 dilution

Lane 1:

Wild-type A549 cell lysates at 20 µg

Lanes 2 and 4:

Empty

Lane 3:

KRT18 knockout A549 cell lysates at 20 µg

Lane 5:

HeLa cell lysates at 20 µg

Lane 6:

SH-SY5Y cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-ARPC5L antibody [EPR10274] ab169763 staining at 1/5000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 18 kDa in Wild-type HEK-293T cell line with no signal observed at this size in ARPC5L knockout HEK-293T cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-ARPC5L antibody [EPR10274] (<a href='/products/primary-antibodies/arpc5l-antibody-epr10274-ab169763'>ab169763</a>) at 1/5000 dilution

Lane 1:

Wild-type HEK-293T cell lysates at 20 µg

Lane 2:

Western blot - Human ARPC5L knockout HEK-293T cell line (<a href='/products/cell-lines/human-arpc5l-knockout-hek-293t-cell-line-ab266399'>ab266399</a>) at 20 µg

Lane 3:

MCF7 cell lysates at 20 µg

Lane 4:

A549 cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 16 kDa

Observed band size: 18 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade ab108341 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 100 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in AR knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (<a href='/products/primary-antibodies/androgen-receptor-antibody-er1792-chip-grade-ab108341'>ab108341</a>) at 1/1000 dilution

Lane 1:

Wild-type MCF7 cell lysates at 40 µg

Lane 2:

Western blot - Human AR knockout MCF7 cell line (<a href='/products/cell-lines/human-ar-knockout-mcf7-cell-line-ab286334'>ab286334</a>) at 40 µg

Lane 3:

THP-1 cell lysates at 20 µg

Lane 4:

PC-3 cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 99 kDa

Observed band size: 100 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-MCPIP1 antibody [EPR26062-59] ab315877 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 70 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in ZC3H12A knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-MCPIP1 antibody [EPR26062-59] (<a href='/products/primary-antibodies/mcpip1-antibody-epr26062-59-ab315877'>ab315877</a>) at 1/1000 dilution

Lane 1:

Wild-type MCF7 cell lysates at 40 µg

Lane 2:

ZC3H12A knockout MCF7 cell lysates at 40 µg

Lane 3:

THP-1 cell lysates at 20 µg

Lane 4:

HeLa cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 66 kDa

Observed band size: 70 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-STIP1/STI1 antibody [EPR6606] ab126753 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 63 kDa in Wild-type HAP1 cell line with no signal observed at this size in STIP1 knockout HAP1 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-STIP1/STI1 antibody [EPR6606] (<a href='/products/primary-antibodies/stip1-sti1-antibody-epr6606-ab126753'>ab126753</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 cell lysates at 20 µg

Lanes 2 and 4:

Empty

Lane 3:

STIP1 knockout HAP1 cell lysates at 20 µg

Lane 5:

A431 cell lysates at 20 µg

Lane 6:

HEK-293 cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 63 kDa

Observed band size: 63 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-Met (c-Met) antibody [SP44] - C-terminal ab227637 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 50, 140 kDa in Wild-type HeLa cell line with no signal observed at this size in MET (C-MET) knockout HeLa cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Met (c-Met) antibody [SP44] - C-terminal (<a href='/products/primary-antibodies/met-c-met-antibody-sp44-c-terminal-ab227637'>ab227637</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysates at 20 µg

Lane 2:

Western blot - Human MET (Met (c-Met)) knockout HeLa cell line (<a href='/products/cell-lines/human-met-met-c-met-knockout-hela-cell-line-ab265961'>ab265961</a>) at 20 µg

Lane 3:

T-47D cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 35-55 kDa,145-175 kDa

Observed band size: 50 kDa,140 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-SMARCD2 antibody [EPR20860-251] ab220164 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 59 kDa in Wild-type A549 cell line with no signal observed at this size in SMARCD2 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SMARCD2 antibody [EPR20860-251] (<a href='/products/primary-antibodies/smarcd2-antibody-epr20860-251-ab220164'>ab220164</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysates at 20 µg

Lane 2:

SMARCD2 knockout A549 cell lysates at 20 µg

Lane 3:

Raji cell lysates at 20 µg

Lane 4:

THP-1 cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 59 kDa

Observed band size: 59 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-Cytokeratin 18 antibody [RM1051] ab315096 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 48 kDa in Wild-type A549 cell line with no signal observed at this size in KRT18 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Cytokeratin 18 antibody [RM1051] (<a href='/products/primary-antibodies/cytokeratin-18-antibody-rm1051-ab315096'>ab315096</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysates at 20 µg

Lanes 2 and 4:

Empty

Lane 3:

KRT18 knockout A549 cell lysates at 20 µg

Lane 5:

HeLa cell lysates at 20 µg

Lane 6:

SH-SY5Y cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-CD55 antibody [EPR6689] ab133684 staining at 1/5000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. Samples were not boiled before loading onto the gel. A band was observed at 68-75 kDa in Wild-type MCF7 Not Boiled cell line with no signal observed at this size in CD55 knockout MCF7 Not Boiled cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CD55 antibody [EPR6689] (<a href='/products/primary-antibodies/cd55-antibody-epr6689-ab133684'>ab133684</a>) at 1/5000 dilution

Lane 1:

Wild-type MCF7 Not Boiled cell lysates at 20 µg

Lane 2:

Western blot - Human CD55 knockout MCF7 cell line (<a href='/products/cell-lines/human-cd55-knockout-mcf7-cell-line-ab269494'>ab269494</a>) at 20 µg

Lane 3:

HeLa Not Boiled cell lysates at 20 µg

Lane 4:

LNCaP Not Boiled cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 68-75 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-PAI2 antibody [HL2218] ab317166 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 47 kDa in Wild-type U-87 MG cell line with no signal observed at this size in SERPINB2 knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PAI2 antibody [HL2218] (<a href='/products/primary-antibodies/pai2-antibody-hl2218-ab317166'>ab317166</a>) at 1/1000 dilution

Lane 1:

Wild-type U-87 MG cell lysates at 20 µg

Lanes 2 and 4:

Empty

Lane 3:

Western blot - Human SERPINB2 knockout U-87 MG cell line (<a href='/products/cell-lines/human-serpinb2-knockout-u-87-mg-cell-line-ab306850'>ab306850</a>) at 20 µg

Lane 5:

A431 cell lysates at 20 µg

Lane 6:

HDLM-2 cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-GGCX antibody [EPR11632] ab170921 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. Samples were not boiled before loading onto the gel. A band was observed at 79 kDa in Wild-type HeLa Not Boiled cell line with no signal observed at this size in GGCX knockout HeLa ab265204 Not Boiled cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-GGCX antibody [EPR11632] (<a href='/products/primary-antibodies/ggcx-antibody-epr11632-ab170921'>ab170921</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa Not Boiled cell lysates at 20 µg

Lane 2:

Western blot - Human GGCX knockout HeLa cell line (<a href='/products/cell-lines/human-ggcx-knockout-hela-cell-line-ab265204'>ab265204</a>) at 20 µg

Lane 3:

MCF7 Not Boiled cell lysates at 20 µg

Lane 4:

SH-SY5Y Not Boiled cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 79 kDa

Observed band size: 79 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-TIMM17A/TIM17 antibody [EPR16098] ab192246 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 18 kDa in Wild-type HEK-293T cell line with no signal observed at this size in TIMM17A knockout HEK-293T cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] (<a href='/products/primary-antibodies/timm17a-tim17-antibody-epr16098-ab192246'>ab192246</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysates at 20 µg

Lane 2:

Western blot - Human TIMM17A (TIM17) knockout HEK-293T cell line (<a href='/products/cell-lines/human-timm17a-tim17-knockout-hek-293t-cell-line-ab266305'>ab266305</a>) at 20 µg

Lane 3:

HeLa cell lysates at 20 µg

Lane 4:

MCF7 cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-AHR antibody [BLR118H] - BSA free ab314060 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 96 kDa in Wild-type MCF7 cell line with no signal observed at this size in AHR knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-AHR antibody [BLR118H] - BSA free (<a href='/products/primary-antibodies/ahr-antibody-blr118h-bsa-free-ab314060'>ab314060</a>) at 1/1000 dilution

Lane 1:

Wild-type MCF7 cell lysates at 20 µg

Lane 2:

AHR knockout MCF7 cell lysates at 20 µg

Lane 3:

HeLa cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 96 kDa

Observed band size: 96 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-AHR antibody [HL1984] ab308215 staining at 1/500 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 96 kDa in Wild-type MCF7 cell line with no signal observed at this size in AHR knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-AHR antibody [HL1984] - BSA and Azide free (<a href='/products/primary-antibodies/ahr-antibody-hl1984-ab308215'>ab308215</a>) at 1/500 dilution

Lane 1:

Wild-type MCF7 cell lysates at 20 µg

Lane 2:

AHR knockout MCF7 cell lysates at 20 µg

Lane 3:

HeLa cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 96 kDa

Observed band size: 96 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-IDUA antibody antibody [EPR26926-251] ab323701 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 73 kDa in Wild-type A549 cell line with no signal observed at this size in IDUA knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-IDUA antibody antibody [EPR26926-251] (<a href='/products/primary-antibodies/idua-antibody-antibody-epr26926-251-ab323701'>ab323701</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysates at 20 µg

Lane 2:

IDUA knockout A549 cell lysates at 20 µg

Lane 3:

LNCaP cell lysates at 20 µg

Lane 4:

JEG-3 cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-CD146 antibody [EPR3208] ab75769 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. Samples were not boiled before loading onto the gel. A band was observed at 120 kDa in Wild-type HAP1 Not Boiled cell line with no signal observed at this size in MCAM knockout HAP1 Not Boiled cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CD146 antibody [EPR3208] (<a href='/products/primary-antibodies/cd146-antibody-epr3208-ab75769'>ab75769</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 Not Boiled cell lysates at 40 µg

Lanes 2 and 4:

Empty

Lane 3:

MCAM knockout HAP1 Not Boiled cell lysates at 40 µg

Lane 5:

A-375 Not Boiled cell lysates at 20 µg

Lane 6:

LNCaP Not Boiled cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 72 kDa

Observed band size: 120 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-Axl antibody [EPR19880] ab219651 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 140 kDa in Wild-type A549 cell line with no signal observed at this size in AXL knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Axl antibody [EPR19880] (<a href='/products/primary-antibodies/axl-antibody-epr19880-ab219651'>ab219651</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysates at 20 µg

Lane 2:

Western blot - Human AXL knockout A549 cell line (<a href='/products/cell-lines/human-axl-knockout-a549-cell-line-ab273744'>ab273744</a>) at 20 µg

Lane 3:

HeLa cell lysates at 20 µg

Lane 4:

Jurkat cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 135 kDa

Observed band size: 140 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-Lamin B2 antibody [EPR9701(B)] ab151735 staining at 1/2000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 68 kDa in Wild-type HeLa cell line with no signal observed at this size in LMNB2 knockout HeLa cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Lamin B2 antibody [EPR9701(B)] (<a href='/products/primary-antibodies/lamin-b2-antibody-epr9701b-ab151735'>ab151735</a>) at 1/2000 dilution

Lane 1:

Wild-type HeLa cell lysates at 20 µg

Lanes 2 and 4:

Empty

Lane 3:

Western blot - Human LMNB2 knockout HeLa cell line (<a href='/products/cell-lines/human-lmnb2-knockout-hela-cell-line-ab274914'>ab274914</a>) at 20 µg

Lane 5:

HepG2 cell lysates at 20 µg

Lane 6:

Jurkat cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-Rab11A antibody [EPR7587(B)] ab128913 staining at 1/2000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 24 kDa in Wild-type A549 cell line with no signal observed at this size in RAB11A knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Rab11A antibody [EPR7587(B)] (<a href='/products/primary-antibodies/rab11a-antibody-epr7587b-ab128913'>ab128913</a>) at 1/2000 dilution

Lane 1:

Wild-type A549 cell lysates at 20 µg

Lane 2:

RAB11A knockout A549 cell lysates at 20 µg

Lane 3:

LNCaP cell lysates at 20 µg

Lane 4:

HeLa cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Lane 1 : Wild-type HCT116 cell lysate 20 ug
Lane 2 : CTNNB1 knockout HCT116 cell lysate 20 ug
Lanes 1 - 2 : Merged signal (red and green). Green - ab223075 observed at 95 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab223075 was shown to react with Anti-beta Catenin in wild-type HCT 116 cells in western blot with loss of signal observed in CTNNB1 knockout cell line ab273712 (CTNNB1 knockout cell lysate ab275247). HCT 116 wild-type and CTNNB1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluoroscent western blot (TBS-based) blocking solution 50% (v/v) in TBS-T (0.1% Tween^®) before incubation with ab223075 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4^°C at 1 ug/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-beta Catenin antibody [IGX4794R-3] (<a href='/products/primary-antibodies/beta-catenin-antibody-igx4794r-3-ab223075'>ab223075</a>) at 1 µg/mL

Lane 1:

Wild-type HCT116 cell lysate at 20 µg

Lane 2:

Western blot - Human CTNNB1 (beta Catenin) knockout HCT116 cell lysate (<a href='/products/cell-lysates/human-ctnnb1-beta-catenin-knockout-hct116-cell-lysate-ab275247'>ab275247</a>) at 20 µg

Predicted band size: 85 kDa

Observed band size: 95 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-SSB antibody [EPR6570] ab124932 staining at 1/10000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 49 kDa in Wild-type HeLa cell lysates with no signal observed at this size in SSB knockout HeLa cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SSB antibody [EPR6570] (<a href='/products/primary-antibodies/ssb-antibody-epr6570-ab124932'>ab124932</a>) at 1/10000 dilution

Lane 1:

Wild-type HeLa cell lysates at 20 µg

Lane 2:

Western blot - Human SSB knockout HeLa cell line (<a href='/products/cell-lines/human-ssb-knockout-hela-cell-line-ab274949'>ab274949</a>) at 20 µg

Lane 3:

HCT 116 cell lysates at 20 µg

Lane 4:

PC-3 cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 47 kDa

Observed band size: 49 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Anti-CHEK1 antibody [EP691Y] (ab40866) staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab40866 was shown to bind specifically to CHEK1. A band was observed at 57 kDa in wild-type A549 cell lysates with a reduction in signal observed at this size in CHEK1 heterozygous knockout cell line. To generate this image, wild-type and CHEK1 heterozygous knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Chk1 antibody [EP691Y] (<a href='/products/primary-antibodies/chk1-antibody-ep691y-ab40866'>ab40866</a>) at 1/10000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

CHEK1 knockout A549 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

MOLT-4 cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 54 kDa

Observed band size: 57 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

All lanes:

Western blot - Anti-Chk1 antibody [E250] (<a href='/products/primary-antibodies/chk1-antibody-e250-ab32531'>ab32531</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

CHEK1 knockout A549 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

MOLT-4 cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 54 kDa

Observed band size: 57 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-PRKCD antibody [EPR17075] (ab182126) staining at 1/5000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab182126 was shown to bind specifically to PRKCD. A band was observed at 75-80 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in PRKCD knockout cell line. To generate this image, wild-type and PRKCD knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-PKC delta antibody [EPR17075] (<a href='/products/primary-antibodies/pkc-delta-antibody-epr17075-ab182126'>ab182126</a>) at 1/5000 dilution

Lane 1:

Wild-type U-87 MG cell lysate at 20 µg

Lane 2:

PRKCD knockout U-87 MG cell lysate at 20 µg

Secondary

Lanes 1 - 2:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 2:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 75-80 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-STK11 antibody [EPR19379] (ab199970) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab199970 was shown to bind specifically to STK11. A band was observed at 50-60 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in STK11 knockout cell line. To generate this image, wild-type and STK11 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-LKB1 antibody [EPR19379] (<a href='/products/primary-antibodies/lkb1-antibody-epr19379-ab199970'>ab199970</a>) at 1/1000 dilution

Lane 1:

Wild-type HCT 116 cell lysate at 20 µg

Lane 2:

STK11 knockout HCT 116 cell lysate at 20 µg

Lane 3:

Wild-type HEK-293T ab255553 cell lysate at 20 µg

Lane 4:

STK11 knockout HEK-293T ab261047 cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 50-60 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-BRD3 antibody [EPR23743-226] (ab300106) staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab300106 was shown to bind specifically to BRD3.

A band was observed at 90-100 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in BRD3 knockout cell line.

To generate this image, wild-type and BRD3 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-BRD3 antibody [EPR23743-226] (<a href='/products/primary-antibodies/brd3-antibody-epr23743-226-ab300106'>ab300106</a>) at 1/500 dilution

Lane 1:

Wild-type HCT 116 cell lysate at 20 µg

Lane 2:

BRD3 knockout HCT 116 cell lysate at 20 µg

Lane 3:

Wild-type HEK-293T ab255553 cell lysate at 20 µg

Lane 4:

BRD3 knockout HEK-293T ab260529 cell lysate at 20 µg

Secondary

Lanes 1 - 4:

HRP conjugated Goat anti-Rabbit (H+L) at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 90-100 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Anti-BACE1 antibody [EPR3956] (ab108394) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab108394 was shown to bind specifically to BACE1. A band was observed at 56 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in BACE1 knockout cell line. To generate this image, wild-type and BACE1 knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-BACE1 antibody [EPR3956] (<a href='/products/primary-antibodies/bace1-antibody-epr3956-ab108394'>ab108394</a>) at 1/1000 dilution

Lane 1:

Wild-type U-87 MG cell lysate at 20 µg

Lane 2:

BACE1 knockout U-87 MG cell lysate at 20 µg

Lane 3:

Wild-type SH-SY5Y cell lysate at 20 µg

Lane 4:

BACE1 knockout SH-SY5Y <a href='/products/unavailable/human-bace1-knockout-sh-sy5y-cell-line-ab280078'>ab280078</a> cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 56 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [6C5] - Loading Control (AB8245)

Western blot : Rabbit Monoclonal [EP1254] to MMP9 76003 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 90 kDa in Wild-type A549 TPA-treated (80nM, 24h) cell lysates with no signal observed at this size in MMP9 knockout A549 TPA-treated (80nM, 24h) cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-MMP9 antibody [EP1254] (<a href='/products/primary-antibodies/mmp9-antibody-ep1254-ab76003'>ab76003</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 TPA-treated (80nM, 24h) at 20 µg

Lane 2:

Wild-type A549 Vehicle Control TPA (0nM, 24h) at 20 µg

Lanes 3 - 4:

Western blot - Human MMP9 knockout A549 cell line (ab286527) at 20 µg

Lane 5:

Human Lung at 20 µg

Lane 6:

MOLT-4 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 90 kDa

false