Anti-GAP43 抗体 - Neuronal Marker (ab12274)
Key features and details
- Rabbit polyclonal to GAP43 - Neuronal Marker
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GAP43 antibody - Neuronal Marker
GAP43 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GAP43 - Neuronal Marker -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) corresponding to Cat GAP43.
Database link: Q6S9D9 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12274の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (2) |
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. -
配列類似性
Belongs to the neuromodulin family.
Contains 1 IQ domain. -
翻訳後修飾
Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. -
細胞内局在
Cell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes. - Information by UniProt
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参照データベース
- Entrez Gene: 2596 Human
- GenBank: NP_002036 Human
- Omim: 162060 Human
- SwissProt: P17677 Human
- Unigene: 134974 Human
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別名
- Axonal membrane protein GAP 43 antibody
- Axonal membrane protein GAP-43 antibody
- B 50 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody - Neuronal Marker (ab12274)Ab12274 staining human neuropil. Staining is localized to the cytoplasm and membrane.
Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, citrate pH 6.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (28)
ab12274 は 28 報の論文で使用されています。
- Zhang XQ et al. C-X-C chemokine receptor type 7 antibody enhances neural plasticity after ischemic stroke. Neural Regen Res 18:1976-1982 (2023). PubMed: 36926722
- Cao SQ et al. P. edulis Extract Protects Against Amyloid-β Toxicity in Alzheimer's Disease Models Through Maintenance of Mitochondrial Homeostasis via the FOXO3/DAF-16 Pathway. Mol Neurobiol 59:5612-5629 (2022). PubMed: 35739408
- Liu YP et al. Schwann cells-derived exosomal miR-21 participates in high glucose regulation of neurite outgrowth. iScience 25:105141 (2022). PubMed: 36204278
- Carroll L et al. Cutaneous expression of growth-associated protein 43 is not a compelling marker for human nerve regeneration in carpal tunnel syndrome. PLoS One 17:e0277133 (2022). PubMed: 36383568
- Li L et al. Proteomic Analysis of Rhesus Macaque Brain Explants Treated With Borrelia burgdorferi Identifies Host GAP-43 as a Potential Factor Associated With Lyme Neuroborreliosis. Front Cell Infect Microbiol 11:647662 (2021). PubMed: 34178719
