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AB96757

Anti-GANAB 抗体

Anti-GANAB antibody

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(1 Publication)

Rabbit Polyclonal GANAB antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GANAB aa 50-300.

別名を表示する

G2AN, KIAA0088, GANAB, Neutral alpha-glucosidase AB, Alpha-glucosidase 2, Glucosidase II subunit alpha

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-GANAB antibody (AB96757)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GANAB antibody (AB96757)

Mehanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells staibed forGANAB using ab96757 at1/500 dilution (green) in ICC/IF. Alpha tubulin filaments are detected with an anti-Alpha tubulin antibody (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody (AB96757)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody (AB96757)

Paraffin-embedded human breast carcinoma tissue stained for GANAB using ab96757 at 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunocytochemistry/ Immunofluorescence - Anti-GANAB antibody (AB96757)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GANAB antibody (AB96757)

Immunofluorescence analysis of GANAB in methanol-fixed HeLa cells, using ab96757 at 1/200 dilution (1) and costained with Hoechst 33342 (2).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody (AB96757)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody (AB96757)

Immunohistochemical analysis of GANAB in paraffin-embedded SW480 xenograft, using ab96757 at 1/500 dilution.

Western blot - Anti-GANAB antibody (AB96757)
  • WB

Unknown

Western blot - Anti-GANAB antibody (AB96757)

7.5% SDS PAGE

All lanes:

Western blot - Anti-GANAB antibody (ab96757) at 1/1000 dilution

All lanes:

NIH-3T3 whole cell lysate at 30 µg

Predicted band size: 107 kDa

false

Western blot - Anti-GANAB antibody (AB96757)
  • WB

Supplier Data

Western blot - Anti-GANAB antibody (AB96757)

All lanes:

Western blot - Anti-GANAB antibody (ab96757) at 1/1000 dilution

Lane 1:

Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 30 µg

Lane 2:

GANAB knockout HeLa cell extract at 30 µg

Secondary

All lanes:

Anti-Rabbit IgG HRP

Predicted band size: 107 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

WB, IHC-P, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human GANAB aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

Q14697

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代替製品

Primary Antibodies

AB176349

Anti-GANAB antibody [EPR12376]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody [EPR12376] (AB176349)

  • 0
  • 0
Primary Antibodies

AB179805

Anti-GANAB antibody [EPR12377(B)]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-GANAB antibody [EPR12377(B)] (AB179805)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB22595

Anti-Calnexin antibody - ER Marker

Lab Essentials|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - ER Marker (AB22595)

  • 5
  • 28
Primary Antibodies

AB124879

Anti-UGGT/UGT1 antibody [EPR5105]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGGT/UGT1 antibody [EPR5105] (AB124879)

  • 0
  • 0
Primary Antibodies

AB133636

Anti-CHX10 antibody [EPR7022]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CHX10 antibody [EPR7022] (AB133636)

  • 0
  • 0
Primary Antibodies

AB108931

Anti-HAUSP / USP7 antibody [EPR4253]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-HAUSP / USP7 antibody [EPR4253] (AB108931)

  • 4
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GANAB also known as Glucosidase II alpha subunit or neutral alpha-glucosidase AB is an enzyme with an approximate mass of 110 kDa. It functions as part of the endoplasmic reticulum's quality control system modifying N-linked glycans on newly synthesized glycoproteins. GANAB cleaves glucose residues facilitating proper folding and maturation of glycoproteins. Expression of GANAB occurs mainly in the endoplasmic reticulum of various cell types indicating its broad relevance in cellular protein processing.
Biological function summary

GANAB performs a critical role in the maintenance of glycoprotein quality. It acts as part of the Glucosidase II complex working alongside a beta subunit to continue glycoprotein processing. Through the removal of glucose from N-glycans GANAB aids in the correct protein folding and prevents accumulation of misfolded proteins. This function is essential for the normal function of the endoplasmic reticulum-associated degradation (ERAD) pathway.

Pathways

Proper glycoprotein folding through GANAB activity integrates into the broader ERAD pathway and the unfolded protein response. GANAB's role complements the activities of other glycosylation-related proteins such as calnexin and calreticulin ensuring only correctly folded proteins advance through the secretory pathway. This integrated network maintains cellular homeostasis and prevents stress conditions in the endoplasmic reticulum.

GANAB's dysfunction associates with autosomal dominant polycystic liver disease and familial hyperinsulinism. These connections emphasize the importance of proper glycoprotein modification managed by GANAB. In these conditions alterations in GANAB or its interacting proteins can lead to aberrant cellular functions and consequent pathologies highlighting potential therapeutic targets in GANAB-associated pathways for disease management.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Catalytic subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins (PubMed : 10929008). Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia (PubMed : 27259053).
See full target information GANAB
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Translational cancer research 8:1975-1984 PubMed35116946

2019

Knockdown of actin-like 8 inhibits cell proliferation by regulating FOXM1, STMN1, PLK1, and BIRC5 in lung adenocarcinoma A549 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shanwu Ma,Guangliang Qiang,Weipeng Shao,Chaoyang Liang,Xiaowei Wang,Deruo Liu
View all publications
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Abcam product promise

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