Anti-gamma Tubulin 抗体 [GTU-88] - Centrosome Marker (ab11316)
Key features and details
- Mouse monoclonal [GTU-88] to gamma Tubulin - Centrosome Marker
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Chicken, Cow, Dog, Human, African green monkey, Chinese hamster
- Isotype: IgG1
製品の概要
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製品名
Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker
gamma Tubulin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [GTU-88] to gamma Tubulin - Centrosome Marker -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Chicken, Cow, Dog, Human, African green monkey, Chinese hamster -
免疫原
Synthetic peptide conjugated to KLH, corresponding to N-terminal amino acids 38-53 of gamma Tubulin.
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特記事項
This product was changed from ascites to tissue culture supernatant on 18th October 2016. The following lots are from ascites and are still in stock as of 18th October 2016 (GR286332 and GR225007). Lot numbers higher than GR286332 will be from tissue culture supernatant. Please note that dilutions may need to be adjusted accordingly.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
Purified from Tissue Culture Supernatant. -
ポリ/モノ
モノクローナル -
クローン名
GTU-88 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11316の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (10) |
Use a concentration of 1 - 2 µg/ml.
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WB | (6) |
1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 1 - 2 µg/ml. |
WB
1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
ターゲット情報
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機能
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. -
配列類似性
Belongs to the tubulin family. -
翻訳後修飾
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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参照データベース
- Entrez Gene: 540216 Cow
- Entrez Gene: 541101 Cow
- Entrez Gene: 403695 Dog
- Entrez Gene: 490962 Dog
- Entrez Gene: 27175 Human
- Entrez Gene: 7283 Human
- Entrez Gene: 103733 Mouse
- Entrez Gene: 103768 Mouse
see all -
別名
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
see all
画像
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All lanes : Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) at 1/10000 dilution
Lane 1 : HeLa cell line lysates
Lane 2 : U87 cell line lysates
Lane 3 : COS7 cell line lysates
Lane 4 : P19 cell line lysates
Lane 5 : Hepa1-6 cell line lysates
Lane 6 : CHO cell line lysates
Lane 7 : MDBK cell line lysates
Lane 8 : MDCK cell line lysates
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase
Predicted band size: 48 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)
ab11316 at a 1/200 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy3 conjugated Goat anti-Mouse (H+L) (green stain). The antibody recognizes gamma-tubulin located at the centrosomes in metaphase and also faintly recognizes the centrosome association during interphase.
This image is courtesy of an Abreview submitted by Kirk McManus on 2 December 2005.
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Lane 1 : Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) at 1/20000 dilution
Lane 2 : Negative control (secondary antibody only)
All lanes : Whole cell extract of chicken fibroblasts
Secondary
All lanes : Alkaline Phosphatase conjugated goat anti-mouse IgG
Predicted band size: 48 kDa
Observed band size: 48 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin using ab11316 at a dilution of 1/5000. Cells were permeabilized with methanol and folowed by fixation with acetone. A Cy3-conjugated goat anti-mouse IgG was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (178)
ab11316 は 178 報の論文で使用されています。
- Khatri D et al. Deficiency of the minor spliceosome component U4atac snRNA secondarily results in ciliary defects in human and zebrafish. Proc Natl Acad Sci U S A 120:e2102569120 (2023). PubMed: 36802443
- Tsekitsidou E et al. Calcineurin associates with centrosomes and regulates cilia length maintenance. J Cell Sci 136:N/A (2023). PubMed: 37013443
- Bieder A et al. Genetic and protein interaction studies between the ciliary dyslexia candidate genes DYX1C1 and DCDC2. BMC Mol Cell Biol 24:20 (2023). PubMed: 37237337
- Wang L et al. RBM4 regulates cellular senescence via miR1244/SERPINE1 axis. Cell Death Dis 14:27 (2023). PubMed: 36639375
- Hildebrandt RP et al. Muscleblind-like proteins use modular domains to localize RNAs by riding kinesins and docking to membranes. Nat Commun 14:3427 (2023). PubMed: 37296096