Anti-gamma Tubulin 抗体 [EPR16793] - Centrosome Marker
Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker
- RabMAb
- Recombinant
- Lab Essentials
- 20ul selling size
- 詳細を見る
4
(3 Reviews)
|
(37 Publications)
Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (ab179503) is a rabbit monoclonal antibody for Western Blot, Flow Cytometry (Intra), IP, IHC-P, ICC/IF. Suitable for Chicken, Cow, Dog, Hamster, Human, Mouse, Rat, Monkey, Xenopus, Zebrafish.
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications
別名を表示する
Tubulin gamma-2 chain, Gamma-2-tubulin, TUBG2
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (AB179503)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling gamma Tubulin with ab179503 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining on tumor cells of breast carcinoma is observed. Counter stained with Hematoxylin.
Negative control : Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (AB179503)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling gamma Tubulin with ab179503 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasmic and centrosome staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab179503 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
- IP
Supplier Data
Immunoprecipitation - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (AB179503)
Gamma Tubulin was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab179503 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab179503 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution. Lane 1 : HeLa whole cell extract. Lane 2 : PBS instead of HeLa whole cell extract.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (ab179503)
Predicted band size: 51 kDa
false
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (AB179503)
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling gamma Tubulin with ab179503 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on epithelial cells of rat testis is observed. Counter stained with Hematoxylin.
Negative control : Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (AB179503)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling gamma Tubulin with ab179503 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining on epithelial cells of mouse testis is observed. Counter stained with Hematoxylin.
Negative control : Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Supplier Data
Western blot - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (AB179503)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (ab179503) at 1/2000 dilution
Lane 1:
HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg
Lane 2:
MDCK (Canine kidney cell line) whole cell lysates at 20 µg
Lane 3:
MDBK (Bovine kidney cell line) whole cell lysates at 20 µg
Lane 4:
COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates at 20 µg
Lane 5:
Zebrafish lysates at 20 µg
Lane 6:
Xenopus (X. tropicalis) muscle lysates at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 48 kDa
false
- WB
Supplier Data
Western blot - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (AB179503)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (ab179503) at 1/20000 dilution
All lanes:
Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 48 kDa
false
- WB
Supplier Data
Western blot - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (AB179503)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (ab179503) at 1/2000 dilution
Lane 1:
Human fetal brain lysates at 10 µg
Lane 2:
Human fetal heart lysates at 10 µg
Lane 3:
Human fetal kidney lysates at 10 µg
Secondary
All lanes:
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 48 kDa
false
- WB
Supplier Data
Western blot - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (AB179503)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (ab179503) at 1/2000 dilution
Lane 1:
Mouse brain lysates at 10 µg
Lane 2:
Mouse spleen lysates at 10 µg
Lane 3:
Rat brain lysates at 10 µg
Lane 4:
Rat heart lysates at 10 µg
Lane 5:
Rat kidney lysates at 10 µg
Lane 6:
C6 (Rat glial tumor cells) whole cell lysates at 10 µg
Lane 7:
RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates at 10 µg
Lane 8:
PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 10 µg
Lane 9:
NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 48 kDa
false
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (AB179503)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) cells labeling gamma Tubulin with ab179503 at 1/80 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
- WB
Supplier Data
Western blot - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (AB179503)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (ab179503) at 1/20000 dilution
Lane 1:
UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates at 20 µg
Lane 2:
BHK (Hamster kidney fibroblast cells) whole cell lysates at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 48 kDa
false
関連する標識済み抗体及び組成の異なる製品 (3)
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Anti-gamma Tubulin antibody [EPR16793] - BSA and Azide free
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-gamma Tubulin - Centrosome Marker antibody [EPR16793]
Reactivity data
製品の詳細
What is this antibody validated in?
Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (ab179503) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in African green monkey, Chicken, Cow, Dog, Hamster, Human, Mouse, Rat, Xenopus tropicalis, Zebrafish samples.
What is the molecular weight of gamma Tubulin?
Anti-gamma Tubulin [EPR16793] - Centrosome Marker (ab179503) specifically detects a band for gamma Tubulin (UniProt: P23258) at a molecular weight of 51kDa.
Trusted by the scientific community
Anti-gamma Tubulin [EPR16793] - Centrosome Marker (ab179503) was first used in a scientific publication in 2014 and has been cited over 20 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR16793] also available for your convenience: ab179503, Alexa Fluor® 647 - ab314509
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
出荷温度及び保存条件
製品の状態
精製方法
バッファー組成
出荷温度
短期保存期間
短期保存温度
長期保存温度
分注に関する情報
保管に関する情報
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Gamma-tubulin is part of the gamma-tubulin ring complex also known as gamma-TuRC which anchors the microtubule minus ends influencing microtubule stability and organization. This complex functions as a nucleating template for microtubules which are critical for various cell processes including intracellular transport and chromosome separation during cell division. Gamma-tubulin plays an indispensable role in maintaining the correct function of the centrosome an important cellular organelle in cell cycle progression.
Pathways
Gamma-tubulin participates in processes involving cell cycle regulation and mitotic spindle formation. These processes depend heavily on microtubule dynamics and organization. Gamma-tubulin interacts closely with proteins like centrins and pericentrin within these pathways. Such interactions ensure accurate chromosomal segregation during mitosis highlighting its importance in cellular reproduction and stability.
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文献 (37)
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