Anti-gamma Tubulin 抗体

• ICC

Lab

Immunocytochemistry - Anti-gamma Tubulin antibody - Centrosome Marker (AB11321)

ICC/IF image of ab11321 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11321, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.

• ICC

Supplier Data

Immunocytochemistry - Anti-gamma Tubulin antibody - Centrosome Marker (AB11321)

Immunocytochemistry analysis of HeLa cells labeling gamma Tubulin with ab11321 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, Atto-488 conjugate. Cells were fixed and permeabilized with methanol followed by acetone.

• ICC

Supplier Data

Immunocytochemistry - Anti-gamma Tubulin antibody - Centrosome Marker (AB11321)

Immunocytochemistry analysis of Rat 1 cells labeling gamma Tubulin with ab11321 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, Cy3™ conjugate. Cells were fixed and permeabilized with methanol followed by acetone.

• WB

Supplier Data

Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (AB11321)

All lanes:

Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (ab11321) at 1/1000 dilution

Lane 1:

HeLa cell lysate

Lane 2:

NIH-3T3 cell lysate

Lane 3:

MDCK cell lysate

Lane 4:

CHO cell lysate

Lane 5:

Rat-2 cell lysate

Lane 6:

A431 cell lysate

Lane 7:

Jurkat cell lysate

Lane 8:

U87 cell lysate

Lane 9:

C2C12 cell lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG-peroxidase

Predicted band size: 51 kDa

false

• WB

AbReview56025****

Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (AB11321)

Blocking buffer 5% milk.

All lanes:

Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (ab11321) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg

Lane 2:

Mouse hepatocytes whole cell lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit (HRP) secondary antibody at 1/5000 dilution

Predicted band size: 51 kDa

Observed band size: 50 kDa

true

Exposure time: 5s