Anti-gamma Tubulin 抗体 (ab11317)
Key features and details
- Rabbit polyclonal to gamma Tubulin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human, Chinese hamster
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-gamma Tubulin antibody
gamma Tubulin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to gamma Tubulin -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human, Chinese hamster
交差が予測される動物種: Drosophila melanogaster, Schizosaccharomyces pombe, Sea urchin
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免疫原
Synthetic peptide:
EEFATEGTDRKDVFFY-C
conjugated to KLH, corresponding to N terminal amino acids 38-53 of Human gamma Tubulin -
ポジティブ･コントロール
- ICC/IF: Rat2 cells; NIH3T3 cells; HeLa cells. WB: Rat2 cell lysate; A431 cell lysate; HeLa cell lysate; Jurkat cell lysate; U87 cell lysate; C2C12 cell lysate; CHO cell lysate; NIH3T3 cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
精製度
IgG fraction -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11317の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (5) |
1/5000.
It is recommended to fix cells with methanol/acetone. |
| WB | (7) |
1/1000. Detects a band of approximately 48 kDa. This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen.
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| 特記事項 |
|---|
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ICC/IF
1/5000. It is recommended to fix cells with methanol/acetone. |
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WB
1/1000. Detects a band of approximately 48 kDa. This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen. |
ターゲット情報
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機能
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. -
配列類似性
Belongs to the tubulin family. -
翻訳後修飾
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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参照データベース
- Entrez Gene: 35199 Drosophila melanogaster
- Entrez Gene: 27175 Human
- Entrez Gene: 7283 Human
- Entrez Gene: 103733 Mouse
- Entrez Gene: 103768 Mouse
- Entrez Gene: 252921 Rat
- Entrez Gene: 680991 Rat
- Omim: 191135 Human
see all -
別名
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)
Immunocytochemistry/Immunofluorescence analysis of NIH3T3 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
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Western blot - Anti-gamma Tubulin antibody (ab11317)All lanes : Anti-gamma Tubulin antibody (ab11317) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : C2C12 cell lysate
Lane 5 : Rat2 cell lysate
Lane 6 : U87 cell lysate
Lane 7 : NIH3T3 cell lysate
Lane 8 : CHO cell lysate
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG -
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)Immunocytochemistry/Immunofluorescence analysis of Rat2 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (108)
ab11317 は 108 報の論文で使用されています。
- Muys BR et al. Matrin3 regulates mitotic spindle dynamics by controlling alternative splicing of CDC14B. Cell Rep 42:112260 (2023). PubMed: 36924503
- He S et al. Distinct dynein complexes defined by DYNLRB1 and DYNLRB2 regulate mitotic and male meiotic spindle bipolarity. Nat Commun 14:1715 (2023). PubMed: 36973253
- Chow SE et al. YAP co-localizes with the mitotic spindle and midbody to safeguard mitotic division in lung-cancer cells. FEBS J 290:5704-5719 (2023). PubMed: 37549045
- Esper ME et al. Immunofluorescence Labeling of Skeletal Muscle in Development, Regeneration, and Disease. Methods Mol Biol 2566:113-132 (2023). PubMed: 36152246
- Brand YM et al. A new host-targeted antiviral cyclolignan (SAU-22.107) for Dengue Virus infection in cell cultures. Potential action mechanisms based on cell imaging. Virus Res 323:198995 (2023). PubMed: 36336130