Anti-gamma Synuclein/SNCG 抗体 [EPR26218-79]

• WB

Supplier Data

Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (AB315442)

In Western blot, ab315442 was shown to bind specifically to gamma Synuclein. Target of interest was observed at 15 kDa in wild-type HeLa cell lysates (lane 1) with no signal observed at this size in gamma Synuclein knockout cell line (lane 2) (lane 2, knockout cell line ab265623 / knockout cell lysate ab257694).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HEK-293 (PMID : 12121974).

The identity of the higher MW bands are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442) at 1/1000 dilution

Lane 1:

Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

gamma Synuclein/SNCG knockout HeLa whole cell lysate at 20 µg

Lane 3:

SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 15 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (AB315442)

Negative control : liver (PMID : 16596223).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the higher MW bands are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human hypothalamus tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 15 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (AB315442)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : liver (PMID : 16596223).

The identity of the higher MW bands are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442) at 1/1000 dilution

Lane 1:

Mouse hypothalamus tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Rat cerebellum tissue lysate at 20 µg

Lane 4:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 17 kDa,36 kDa

false

Exposure time: 158s

• WB

Supplier Data

Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (AB315442)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human beta Synuclein and alpha Synuclein.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

In Western blot, Anti-alpha + beta Synuclein antibody [EP1646Y] - (ab51252) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442) at 1/1000 dilution

Lane 1:

His-tagged human gamma Synuclein recombinant protein at 10 ng

Lane 2:

His-tagged human beta Synuclein recombinant protein at 10 ng

Lane 3:

His-tagged human alpha Synuclein recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 14 kDa,20 kDa,19 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (AB315442)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 5 µg

Lane 2:

293T cells transfected with a human gamma Synuclein expression vector containing a myc-His-tag®, whole cell lysate at 5 µg

Lane 3:

293T cells transfected with a mouse gamma Synuclein expression vector containing a myc-His-tag®, whole cell lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 17 kDa,19 kDa,36 kDa

false

Exposure time: 6s