AB251394

Anti-gamma Sarcoglycan 抗体 [EPR17862-7] - BSA and Azide free

Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal gamma Sarcoglycan antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

別名を表示する

Gamma-sarcoglycan, Gamma-SG, 35 kDa dystrophin-associated glycoprotein, 35DAG, SGCG

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

This data was developed using ab203113, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling gamma Sarcoglycan with ab203113 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Cell membrane and weak cytoplasmic staining observed. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody, with secondary ab97051 Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

This data was developed using ab203113, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling gamma Sarcoglycan with ab203113 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. No staining is observed on Human colon tissue. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody, with secondary ab97051 Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Unknown

Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

This data was developed using ab203113, the same antibody clone in a different buffer formulation.

Human gamma Sarcoglycan was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab203113 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203113 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1 : Human fetal kidney whole cell lysate 10 μg (Input). Lane 2 : ab203113 IP in Human fetal kidney whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203113 in Human fetal kidney whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 Seconds

Blocking/Dilution buffer 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] (<a href='/products/primary-antibodies/gamma-sarcoglycan-antibody-epr17862-7-ab203113'>ab203113</a>)

Predicted band size: 32 kDa

false

Supplier Data

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

This data was developed using ab203113, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

This protein is expressed in skeletal and heart muscle.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (<a href='/products/primary-antibodies/gamma-sarcoglycan-antibody-epr17862-7-ab203113'>ab203113</a>) at 1/10000 dilution

All lanes:

Human skeletal muscle whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

This data was developed using ab203113, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

This protein is expressed in skeletal and heart muscle.

ab181602 Anti-GAPDH antibody [EPR16891] control used.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (<a href='/products/primary-antibodies/gamma-sarcoglycan-antibody-epr17862-7-ab203113'>ab203113</a>) at 1/2000 dilution

Lane 1:

Human fetal brain whole tissue lysate at 10 µg

Lane 2:

Human fetal heart whole tissue lysate at 10 µg

Lane 3:

Human fetal kidney whole tissue lysate at 10 µg

Lane 4:

Human fetal spleen whole tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

This data was developed using ab203113, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (<a href='/products/primary-antibodies/gamma-sarcoglycan-antibody-epr17862-7-ab203113'>ab203113</a>) at 1/2000 dilution

All lanes:

MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

This data was developed using ab203113, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (<a href='/products/primary-antibodies/gamma-sarcoglycan-antibody-epr17862-7-ab203113'>ab203113</a>) at 1/2000 dilution

All lanes:

Mouse heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 10s

Lab

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

This data was developed using ab203113, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (<a href='/products/primary-antibodies/gamma-sarcoglycan-antibody-epr17862-7-ab203113'>ab203113</a>) at 1/1000 dilution

All lanes:

Mouse muscle lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 30s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR17862-7

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse, Human

アプリケーション

WB, IHC-P, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

ab251394 is the carrier-free version of ab203113.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Gamma sarcoglycan also known as γ-sarcoglycan is a transmembrane protein with a molecular mass of approximately 35-50 kDa. It plays a mechanical role by stabilizing muscle cell membranes. Filamentous and inelastic it integrates into the dystrophin-associated glycoprotein complex (DGC) within skeletal and cardiac muscles. Cells express gamma sarcoglycan primarily in muscle tissue making it vital for muscle integrity and function.

Biological function summary

Gamma sarcoglycan acts as a component of the sarcoglycan complex part of the larger dystrophin-glycoprotein complex. This complex connects the cytoskeleton of a muscle fiber to the extracellular matrix maintaining structural stability under mechanical stress. Its role is to ensure force transmission while preventing membrane damage along with proteins like dystrophin beta sarcoglycan and delta sarcoglycan. Gamma sarcoglycan's expression is important for proper muscle contraction and resilience.

Pathways

Gamma sarcoglycan associates with the dystrophin-glycoprotein complex pathway contributing to cellular stability and muscle fiber integrity. It also intersects with the actin cytoskeleton signaling pathway where it modulates structural organization. In these pathways it works alongside dystrophin to secure muscle fibers during contraction and relaxation cycles preventing damage from mechanical strain.

Mutations in the gamma sarcoglycan gene lead to limb-girdle muscular dystrophy type 2C (LGMD2C) characterized by progressive muscle weakness. This genetic disorder causes instability in the sarcoglycan complex resulting in muscle fiber degradation. In another condition dilated cardiomyopathy defects in gamma sarcoglycan disrupt heart muscle function. These diseases often involve interactions with proteins like dystrophin and other sarcoglycans which also contribute to muscle deterioration.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.

See full target information SGCG

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:800 PubMed39731111

2024

Role of PCBP2 in regulating nanovesicles loaded with curcumin to mitigate neuroferroptosis in neural damage caused by heat stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Guo,Yizhan Wu,Guangjun Wang,Jiangwei Liu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-gamma Sarcoglycan 抗体 [EPR17862-7] - BSA and Azide free

Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise