AB203113

Anti-gamma Sarcoglycan 抗体 [EPR17862-7]

Anti-gamma Sarcoglycan antibody [EPR17862-7]

RabMAb
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(2 Publications)

Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) is a rabbit monoclonal antibody detecting gamma Sarcoglycan in Western Blot, IP, IHC-P. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

Gamma-sarcoglycan, Gamma-SG, 35 kDa dystrophin-associated glycoprotein, 35DAG, SGCG

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling gamma Sarcoglycan with ab203113 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. No staining is observed on Human colon tissue. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody with secondary ab97051 Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Blocking/Dilution buffer

5% NFDM/TBST.

This protein is expressed in skeletal and heart muscle.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/10000 dilution

All lanes:

Human skeletal muscle whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 15s

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling gamma Sarcoglycan with ab203113 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Cell membrane and weak cytoplasmic staining observed. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody with secondary ab97051 Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Unknown

Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Human gamma Sarcoglycan was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab203113 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203113 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1 : Human fetal kidney whole cell lysate 10 μg (Input). Lane 2 : ab203113 IP in Human fetal kidney whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203113 in Human fetal kidney whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 Seconds

Blocking/Dilution buffer 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)

Predicted band size: 32 kDa

false

Supplier Data

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Blocking/dilution buffer : 5% NFDM/TBST.

This protein is expressed in skeletal and heart muscle.

ab181602 Anti-GAPDH antibody [EPR16891] control used.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/2000 dilution

Lane 1:

Human fetal brain whole tissue lysate at 10 µg

Lane 2:

Human fetal heart whole tissue lysate at 10 µg

Lane 3:

Human fetal kidney whole tissue lysate at 10 µg

Lane 4:

Human fetal spleen whole tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Blocking/dilution buffer

5% NFDM/TBST.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/2000 dilution

All lanes:

MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/2000 dilution

All lanes:

Mouse heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 10s

Lab

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/1000 dilution

All lanes:

Mouse muscle lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 30s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR17862-7

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, IHC-P, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

製品の詳細

What is this antibody validated in?
Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse samples.

What is the molecular weight of gamma Sarcoglycan?
Anti-gamma Sarcoglycan [EPR17862-7] (ab203113) specifically detects a band for gamma Sarcoglycan (UniProt: Q13326) at a molecular weight of 32kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR17862-7] also available for your convenience: ab203113, Carrier free - ab251394, Alexa Fluor® 647 - ab315257

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Gamma sarcoglycan also known as γ-sarcoglycan is a transmembrane protein with a molecular mass of approximately 35-50 kDa. It plays a mechanical role by stabilizing muscle cell membranes. Filamentous and inelastic it integrates into the dystrophin-associated glycoprotein complex (DGC) within skeletal and cardiac muscles. Cells express gamma sarcoglycan primarily in muscle tissue making it vital for muscle integrity and function.

Biological function summary

Gamma sarcoglycan acts as a component of the sarcoglycan complex part of the larger dystrophin-glycoprotein complex. This complex connects the cytoskeleton of a muscle fiber to the extracellular matrix maintaining structural stability under mechanical stress. Its role is to ensure force transmission while preventing membrane damage along with proteins like dystrophin beta sarcoglycan and delta sarcoglycan. Gamma sarcoglycan's expression is important for proper muscle contraction and resilience.

Pathways

Gamma sarcoglycan associates with the dystrophin-glycoprotein complex pathway contributing to cellular stability and muscle fiber integrity. It also intersects with the actin cytoskeleton signaling pathway where it modulates structural organization. In these pathways it works alongside dystrophin to secure muscle fibers during contraction and relaxation cycles preventing damage from mechanical strain.

Mutations in the gamma sarcoglycan gene lead to limb-girdle muscular dystrophy type 2C (LGMD2C) characterized by progressive muscle weakness. This genetic disorder causes instability in the sarcoglycan complex resulting in muscle fiber degradation. In another condition dilated cardiomyopathy defects in gamma sarcoglycan disrupt heart muscle function. These diseases often involve interactions with proteins like dystrophin and other sarcoglycans which also contribute to muscle deterioration.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.

See full target information SGCG

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Methods & clinical development 28:284-299 PubMed36816759

2023

Systemic γ-sarcoglycan AAV gene transfer results in dose-dependent correction of muscle deficits in the LGMD 2C/R5 mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Young-Eun Seo,Stephen H Baine,Amber N Kempton,Oliver C Rogers,Sarah Lewis,Kaitlin Adegboye,Alex Haile,Danielle A Griffin,Ellyn L Peterson,Eric R Pozsgai,Rachael A Potter,Louise R Rodino-Klapac

Molecular therapy. Methods & clinical development 13:494-502 PubMed31194043

2019

An AAV-SGCG Dose-Response Study in a γ-Sarcoglycanopathy Mouse Model in the Context of Mechanical Stress.

Applications

Unspecified application

Species

Unspecified reactive species

David Israeli,Jérémie Cosette,Guillaume Corre,Fatima Amor,Jérôme Poupiot,Daniel Stockholm,Marie Montus,Bernard Gjata,Isabelle Richard

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-gamma Sarcoglycan 抗体 [EPR17862-7]

Anti-gamma Sarcoglycan antibody [EPR17862-7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise