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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-gamma Adaptin 抗体 [EPR9875(B)] (ab167153)

Submit a review Submit a question References (1)
Western blot - Anti-gamma Adaptin antibody [EPR9875(B)] (ab167153)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Adaptin antibody [EPR9875(B)] (ab167153)
  • Western blot - Anti-gamma Adaptin antibody [EPR9875(B)] (ab167153)
  • Anti-gamma Adaptin antibody [EPR9875(B)] (ab167153)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9875(B)] to gamma Adaptin
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-gamma Adaptin antibody [EPR9875(B)]
    gamma Adaptin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR9875(B)] to gamma Adaptin

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide within Human gamma Adaptin (C terminal). The exact sequence is proprietary.

  • ポジティブ･コントロール

    • HeLa and 293T cell lysates. Human lung carcinoma tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20ºC.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR9875(B)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab167153の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 91 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 91 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 機能

    Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

  • 組織特異性

    Widely expressed.

  • 配列類似性

    Belongs to the adaptor complexes large subunit family.
    Contains 1 GAE domain.

  • 細胞内局在

    Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Adapter-related protein complex 1 subunit gamma-1 antibody
    • Adaptor protein complex AP 1 gamma 1 subunit antibody
    • Adaptor protein complex AP 1 subunit gamma 1 antibody
    • Adaptor protein complex AP-1 subunit gamma-1 antibody
    • Adaptor related protein complex 1 gamma 1 subunit antibody
    • ADTG antibody
    • AP 1 complex subunit gamma 1 antibody
    • AP-1 complex subunit gamma-1 antibody
    • AP1G1 antibody
    • AP1G1_HUMAN antibody
    • CLAPG1 antibody
    • Clathrin assembly protein complex 1 gamma 1 large chain antibody
    • Clathrin assembly protein complex 1 gamma large chain antibody
    • Clathrin assembly protein complex 1 gamma-1 large chain antibody
    • Clathrin associated/assembly/adaptor protein large gamma 1 antibody
    • Gamma1 adaptin antibody
    • Gamma1-adaptin antibody
    • Golgi adaptor HA1/AP1 adaptin gamma subunit antibody
    • Golgi adaptor HA1/AP1 adaptin subunit gamma 1 antibody
    • Golgi adaptor HA1/AP1 adaptin subunit gamma-1 antibody
    • MGC18255 antibody
    see all

画像

  • Western blot - Anti-gamma Adaptin antibody [EPR9875(B)] (ab167153)
    Western blot - Anti-gamma Adaptin antibody [EPR9875(B)] (ab167153)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: gamma Adaptin knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Human fetal brain tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab167153 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab167153 was shown to specifically react with gamma Adaptin when gamma Adaptin knockout samples were used. Wild-type and gamma Adaptin knockout samples were subjected to SDS-PAGE. ab167153 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Adaptin antibody [EPR9875(B)] (ab167153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Adaptin antibody [EPR9875(B)] (ab167153)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling gamma Adaptin with ab167153 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-gamma Adaptin antibody [EPR9875(B)] (ab167153)
    Western blot - Anti-gamma Adaptin antibody [EPR9875(B)] (ab167153)
    All lanes : Anti-gamma Adaptin antibody [EPR9875(B)] (ab167153) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 91 kDa

  • Anti-gamma Adaptin antibody [EPR9875(B)] (ab167153)
    Anti-gamma Adaptin antibody [EPR9875(B)] (ab167153)

参考文献 (1)

ab167153 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab167153 は 1 報の論文で使用されています。

  • Decara J  et al. The adiponectin promoter activator NP-1 induces high levels of circulating TNFa and weight loss in obese (fa/fa) Zucker rats. Sci Rep 8:9858 (2018). PubMed: 29959379

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