Anti-gamma Actin 抗体 (ab84479)
Key features and details
- Rabbit polyclonal to gamma Actin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-gamma Actin antibody
gamma Actin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to gamma Actin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide corresponding to Human gamma Actin. A peptide corresponding to residues surrounding amino acid 365 of Human gamma Actin.
Database link: NM_001614 -
ポジティブ･コントロール
- WB: Human SW-620 cell lysates. IHC-P: Human fetal muscle tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84479の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/300 - 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
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| IHC-P |
1/100 - 1/500.
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| 特記事項 |
|---|
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WB
1/300 - 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
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IHC-P
1/100 - 1/500. |
ターゲット情報
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機能
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
関連疾患
Defects in ACTG1 are the cause of deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. -
配列類似性
Belongs to the actin family. -
翻訳後修飾
The methylhistidine determined by Bienvenut et al is assumed to be the tele-methylhistidine isomer by similarity to the mouse ortholog. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 71 Human
- Entrez Gene: 11465 Mouse
- Omim: 102560 Human
- SwissProt: P63261 Human
- SwissProt: P63260 Mouse
- Unigene: 514581 Human
- Unigene: 196173 Mouse
- Unigene: 426706 Mouse
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別名
- ACT antibody
- ACTB antibody
- ACTG antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Actin antibody (ab84479)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal muscle tissue labelling gamma Actin with ab84479 at a dilution of 1/100.
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Western blot - Anti-gamma Actin antibody (ab84479)Anti-gamma Actin antibody (ab84479) at 1/300 dilution + Human SW-620 cell lysates
Predicted band size: 42 kDa
Observed band size: 42 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab84479 は論文での使用が確認できていません。