Anti-galectin 9/Gal-9 抗体 [1G3] (ab153673)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-galectin 9/Gal-9 antibody [1G3] (ab153673)](https://www.abcam.co.jp/ps/products/153/ab153673/Images/ab153673-245169-anti-galectin-9-gal-9-antibody-1g3-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-galectin 9/Gal-9 antibody [1G3] (ab153673)")
Key features and details
- Mouse monoclonal [1G3] to galectin 9/Gal-9
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-galectin 9/Gal-9 antibody [1G3]
galectin 9/Gal-9 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1G3] to galectin 9/Gal-9 -
由来種
Mouse -
特異性
This antibody fails to detect endogenous natural samples in WB.
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human galectin 9/Gal-9 (C terminal). Recombinant fragment produced in E. coli
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ポジティブ･コントロール
- WB: HeLa transfected with galectin 9 / Gal-9 whole cell lysate IHC-P: Human normal Tonsil tissue
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team. -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
1G3 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153673の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 5 µg/ml.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 5 µg/ml. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. -
組織特異性
Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue. -
配列類似性
Contains 2 galectin domains. -
ドメイン
Contains two homologous but distinct carbohydrate-binding domains. -
細胞内局在
Cytoplasm. Secreted. May also be secreted by a non-classical secretory pathway. - Information by UniProt
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参照データベース
- Entrez Gene: 3965 Human
- Omim: 601879 Human
- SwissProt: O00182 Human
- Unigene: 81337 Human
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別名
- 36 kDa beta-galactoside-binding lectin antibody
- Ecalectin antibody
- Gal-9 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-galectin 9/Gal-9 antibody [1G3] (ab153673)
IHC image of galectin 9/Gal-9 staining in Human normal Tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab153673, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - Anti-galectin 9/Gal-9 antibody [1G3] (ab153673)](https://www.abcam.co.jp/ps/products/153/ab153673/Images/ab153673-245166-anti-galectin-9-gal-9-antibody-1g3-western-blot.jpg)
Western blot - Anti-galectin 9/Gal-9 antibody [1G3] (ab153673)All lanes : Anti-galectin 9/Gal-9 antibody [1G3] (ab153673) at 5 µg/ml
Lane 1 : HeLa transfected with galectin 9 / Gal-9 whole cell lysate
Lane 2 : HeLa (wild type control) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG polyclonal antibody (HRP conjugated) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Additional bands at: 18 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (10)
ab153673 は 10 報の論文で使用されています。
- Zhang W et al. Mevalonate improves anti-PD-1/PD-L1 efficacy by stabilizing CD274 mRNA. Acta Pharm Sin B 13:2585-2600 (2023). PubMed: 37425040
- Wang T et al. Integrating cell interaction with transcription factors to obtain a robust gene panel for prognostic prediction and therapies in cholangiocarcinoma. Front Genet 13:981145 (2022). PubMed: 36531216
- Yoshikawa K et al. Prognostic significance of the expression levels of T-cell immunoglobulin mucin-3 and its ligand galectin-9 for relapse-free survival in triple-negative breast cancer. Oncol Lett 23:197 (2022). PubMed: 35572493
- Wang X et al. An Integrated Systematic Analysis and the Clinical Significance of Hepcidin in Common Malignancies of the Male Genitourinary System. Front Genet 13:771344 (2022). PubMed: 35646093
- Curley J et al. Looking past PD-L1: expression of immune checkpoint TIM-3 and its ligand galectin-9 in cervical and vulvar squamous neoplasia. Mod Pathol 33:1182-1192 (2020). PubMed: 32139873
- Xu D et al. PD-L1 Expression Is Regulated By NF-?B During EMT Signaling In Gastric Carcinoma. Onco Targets Ther 12:10099-10105 (2019). PubMed: 31819504
- Schulz H et al. Overall Survival of Ovarian Cancer Patients Is Determined by Expression of Galectins-8 and -9. Int J Mol Sci 19:N/A (2018). IHC-P ; Human . PubMed: 29361803
- Enninga EAL et al. CD206-positive myeloid cells bind galectin-9 and promote a tumor-supportive microenvironment. J Pathol 245:468-477 (2018). PubMed: 29732570
- Feng J et al. Interferon-Stimulated Gene (ISG)-Expression Screening Reveals the Specific Antibunyaviral Activity of ISG20. J Virol 92:N/A (2018). PubMed: 29695422
- Barjon C et al. A novel monoclonal antibody for detection of galectin-9 in tissue sections: application to human tissues infected by oncogenic viruses. Infect Agent Cancer 7:16 (2012). WB, IHC ; Mouse . PubMed: 22805533
