Anti-Galectin 8/Gal-8 抗体 (ab42879)
Key features and details
- Rabbit polyclonal to Galectin 8/Gal-8
- Suitable for: WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Galectin 8/Gal-8 antibody
Galectin 8/Gal-8 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Galectin 8/Gal-8 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human, Recombinant fragment
交差が予測される動物種: Mouse, Rat, Cynomolgus monkey -
免疫原
Synthetic peptide corresponding to Galectin 8/Gal-8 aa 150-250 conjugated to keyhole limpet haemocyanin.
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ポジティブ・コントロール
- This antibody gave a positive signal in Galectin 8/Gal-8 Tagged Recombinant Protein.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab42879の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 35 kDa).
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ICC/IF |
Use a concentration of 5 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 35 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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組織特異性
Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy. -
配列類似性
Contains 2 galectin domains. -
ドメイン
Contains two homologous but distinct carbohydrate-binding domains. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 3964 Human
- Entrez Gene: 56048 Mouse
- Entrez Gene: 116641 Rat
- Omim: 606099 Human
- SwissProt: O00214 Human
- SwissProt: Q9JL15 Mouse
- SwissProt: Q62665 Rat
- Unigene: 4082 Human
see all -
別名
- Gal 8 antibody
- Gal-8 antibody
- Gal8 antibody
see all
画像
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Anti-Galectin 8/Gal-8 antibody (ab42879) at 1 µg/ml + Galectin 8/Gal-8 Recombinant Protein at 0.1 µg
Secondary
Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?ab42879 recognizes the full length tagged recombinant Galectin 8/Gal-8 protein which has an expected molecular weight of 65.5 kDa.
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ICC/IF image of ab42879 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42879, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab42879 は 1 報の論文で使用されています。
- Pied N et al. TBK1 is part of a galectin 8 dependent membrane damage recognition complex and drives autophagy upon Adenovirus endosomal escape. PLoS Pathog 18:e1010736 (2022). PubMed: 35857795