Anti-Galectin 10 抗体 [EPR11197] (ab157475)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 10 antibody [EPR11197] (ab157475)](https://www.abcam.co.jp/ps/products/157/ab157475/Images/ab157475-386552-antigalectin-10-antibody-epr11197-human-colon-carcinoma-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 10 antibody [EPR11197] (ab157475)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11197] to Galectin 10
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Galectin 10 antibody [EPR11197]
Galectin 10 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11197] to Galectin 10 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human spleen, Human bone marrow, Human tonsil and THP-1 lysates; THP-1 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR11197 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab157475の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 16 kDa.
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| ICC/IF |
1/100 - 1/250.
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 16 kDa. |
|
ICC/IF
1/100 - 1/250. |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
May have both lysophospholipase and carbohydrate-binding activities. -
組織特異性
Expressed exclusively by eosinophils and basophils. Not detected in monocytes and neutrophils. -
配列類似性
Contains 1 galectin domain. -
細胞内局在
Cytoplasmic granule. Localized in granules from where it may be secreted or transported to other locations in the cell. - Information by UniProt
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参照データベース
- Entrez Gene: 1178 Human
- Omim: 153310 Human
- SwissProt: Q05315 Human
- Unigene: 889 Human
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別名
- BSF3 antibody
- Charcot Leyden crystal protein antibody
- Charcot-Leyden crystal protein antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 10 antibody [EPR11197] (ab157475)
Positive staining on stromal cells of human colon carcinoma.
The section was incubated with ab157475 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 10 antibody [EPR11197] (ab157475)](https://www.abcam.co.jp/ps/products/157/ab157475/Images/ab157475-386550-antigalectin-10-antibody-epr11197-human-tonsil-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 10 antibody [EPR11197] (ab157475)Positive staining on human tonsil.
The section was incubated with ab157475 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. -
![Western blot - Anti-Galectin 10 antibody [EPR11197] (ab157475)](https://www.abcam.co.jp/ps/products/157/ab157475/Images/ab157475-164543-anti-galectin-10-antibody-epr11197-western-blot.jpg)
Western blot - Anti-Galectin 10 antibody [EPR11197] (ab157475)All lanes : Anti-Galectin 10 antibody [EPR11197] (ab157475) at 1/10000 dilution
Lane 1 : Human spleen lysate
Lane 2 : Human bone marrow lysate
Lane 3 : Human tonsil lysate
Lane 4 : THP-1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 16 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Galectin 10 antibody [EPR11197] (ab157475)](https://www.abcam.co.jp/ps/products/157/ab157475/Images/ab157475-164542-anti-galectin-10-antibody-epr11197-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Galectin 10 antibody [EPR11197] (ab157475)Immunofluorescent analysis of THP-1 cells labeling Galectin 10 with ab157475 at 1/100 dilution. -
![Anti-Galectin 10 antibody [EPR11197] (ab157475)](https://www.abcam.co.jp/ps/products/157/ab157475/Images/ab157475-5-benefits-of-recombinant-antibodies.png)
Anti-Galectin 10 antibody [EPR11197] (ab157475)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab157475 は 1 報の論文で使用されています。
- Delemarre T et al. Type 2 inflammation in chronic rhinosinusitis without nasal polyps: Another relevant endotype. J Allergy Clin Immunol 146:337-343.e6 (2020). PubMed: 32417132