Anti-Galectin 1 抗体 (ab154351)
Key features and details
- Rabbit polyclonal to Galectin 1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Galectin 1 antibody
Galectin 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Galectin 1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Pig, Monkey
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免疫原
Recombinant fragment corresponding to a region within amino acids 1-135 of Human Galectin 1 (P09382).
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ポジティブ･コントロール
- NCI-H929 and BCL-1 whole cell lysate. A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154351の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/5000 - 1/20000. Predicted molecular weight: 15 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/5000 - 1/20000. Predicted molecular weight: 15 kDa. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. -
組織特異性
Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. -
配列類似性
Contains 1 galectin domain. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 326598 Cow
- Entrez Gene: 3956 Human
- Entrez Gene: 16852 Mouse
- Entrez Gene: 414915 Pig
- Entrez Gene: 56646 Rat
- Omim: 150570 Human
- SwissProt: P11116 Cow
- SwissProt: P09382 Human
see all -
別名
- 14 kDa laminin-binding protein antibody
- 14 kDa lectin antibody
- Beta galactoside binding lectin antibody
see all
画像
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Western blot - Anti-Galectin 1 antibody (ab154351)All lanes : Anti-Galectin 1 antibody (ab154351) at 1/500 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : Galectin 1 Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 15 kDa -
Western blot - Anti-Galectin 1 antibody (ab154351)Anti-Galectin 1 antibody (ab154351) at 1/10000 dilution + BCL-1 whole cell lysate at 30 µg
Predicted band size: 15 kDa
15% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody (ab154351)Immunofluorescence analysis of methanol-fixed A431 cells labeling Galectin 1 with ab154351 at 1/200 dilution. Bottom panel shows counterstaining with Hoechst 33342. -
Western blot - Anti-Galectin 1 antibody (ab154351)Anti-Galectin 1 antibody (ab154351) at 1/10000 dilution + NCI-H929 whole cell lysate at 30 µg
Predicted band size: 15 kDa
15% SDS PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab154351 は 2 報の論文で使用されています。
- Qin H et al. Pancreatic Cancer detection via Galectin-1-targeted Thermoacoustic Imaging: validation in an in vivo heterozygosity model. Theranostics 10:9172-9185 (2020). PubMed: 32802185
- Journo G et al. Modulation of Cellular CpG DNA Methylation by Kaposi's Sarcoma-Associated Herpesvirus. J Virol 92:N/A (2018). PubMed: 29899086