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Anti-GALE 抗体 [OTI1C4] (ab118033)

Submit a review Submit a question References (1)
Western blot - Anti-GALE antibody [OTI1C4] (ab118033)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
  • Western blot - Anti-GALE antibody [OTI1C4] (ab118033)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
  • Western blot - Anti-GALE antibody [OTI1C4] (ab118033)

Key features and details

  • Mouse monoclonal [OTI1C4] to GALE
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Dog, Human, African green monkey
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-GALE antibody [OTI1C4]
    GALE 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [OTI1C4] to GALE

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Rat, Dog, Human, African green monkey

  • 免疫原

    Recombinant full length protein corresponding to Human GALE aa 1 to the C-terminus. Produced in HEK-293T cells (NP_000394).
    Database link: Q14376

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: HEK-293T cell lysate transfected with the pCMV6-ENTRY GALE cDNA; HepG2, HeLa, HT-29, A549, COS-7, Jurkat, MDCK, PC-12 and MCF7 cell extracts; Human testis, omentum, uterus, breast, brain, liver, ovary, thyroid gland, colon and spleen extracts. IHC-P: Human breast adenocarcinoma, colon adenocarcinoma, kidney, liver, ovary adenocarcinoma, prostate, prostate carcinoma, bladder carcinoma and lymph node tissues.

  • 特記事項

    The clone number has been updated from 1C4 to OTI1C4, both clone numbers name the same clone.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab118033の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/500 - 1/2000. Predicted molecular weight: 38 kDa.
特記事項
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/500 - 1/2000. Predicted molecular weight: 38 kDa.

ターゲット情報

  • 機能

    Catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine.

  • パスウェイ

    Carbohydrate metabolism; galactose metabolism.

  • 関連疾患

    Defects in GALE are the cause of epimerase-deficiency galactosemia (EDG) [MIM:230350]; also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation. There are two clinically distinct forms of EDG. (1) A benign, or 'peripheral' form with no detectable GALE activity in red blood cells and characterized by mild symptoms. Some patients may suffer no symptoms beyond raised levels of galactose-1-phosphate in the blood. (2) A much rarer 'generalized' form with undetectable levels of GALE activity in all tissues and resulting in severe features such as restricted growth and mental development.

  • 配列類似性

    Belongs to the sugar epimerase family.
  • Information by UniProt

  • 参照データベース

    • 別名

      • FLJ95174 antibody
      • FLJ97302 antibody
      • Galactose 4 epimerase UDP antibody
      • Galactowaldenase antibody
      • galE antibody
      • GALE_HUMAN antibody
      • OTTHUMP00000002991 antibody
      • OTTHUMP00000002994 antibody
      • OTTHUMP00000037931 antibody
      • OTTHUMP00000044857 antibody
      • SDR1E1 antibody
      • short chain dehydrogenase/reductase family 1E member 1 antibody
      • UDP galactose 4 epimerase antibody
      • UDP galactose 4' epimerase antibody
      • UDP glucose 4 epimerase antibody
      • UDP-galactose 4-epimerase antibody
      • UDP-glucose 4-epimerase antibody
      see all

    画像

    • Western blot - Anti-GALE antibody [OTI1C4] (ab118033)
      Western blot - Anti-GALE antibody [OTI1C4] (ab118033)
      All lanes : Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
      Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY GALE cDNA

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 38 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

      Paraffin-embedded human bladder carcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

      Paraffin-embedded human lymph node tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

    • Western blot - Anti-GALE antibody [OTI1C4] (ab118033)
      Western blot - Anti-GALE antibody [OTI1C4] (ab118033)
      All lanes : Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution

      Lane 1 : Human testis extract
      Lane 2 : Human omentum extract
      Lane 3 : Human uterus extract
      Lane 4 : Human breast extract
      Lane 5 : Human brain extract
      Lane 6 : Human liver extract
      Lane 7 : Human ovary extract
      Lane 8 : Human thyroid gland extract
      Lane 9 : Human colon extract
      Lane 10 : Human spleen extract

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 38 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

      Paraffin-embedded human prostate carcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

      Paraffin-embedded human prostate tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

      Paraffin-embedded human ovary adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

      Paraffin-embedded human liver tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

      Paraffin-embedded human kidney tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

      Paraffin-embedded human colon adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (ab118033)

      Paraffin-embedded human breast adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

    • Western blot - Anti-GALE antibody [OTI1C4] (ab118033)
      Western blot - Anti-GALE antibody [OTI1C4] (ab118033)
      All lanes : Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution

      Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
      Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) cell extract
      Lane 4 : A549 (human lung carcinoma cell line) cell extract
      Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
      Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
      Lane 7 : MDCK (Canine kidney cell line) cell extract
      Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract
      Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extract

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 38 kDa

    参考文献 (1)

    ab118033 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab118033 は 1 報の論文で使用されています。

    • Broussard A  et al. Human UDP-galactose 4'-epimerase (GALE) is required for cell-surface glycome structure and function. J Biol Chem 295:1225-1239 (2020). PubMed: 31819007

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