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AB254556

Anti-Galanin 抗体 [EPR22496-308]

Anti-Galanin antibody [EPR22496-308]

4

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal Galanin antibody. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

別名を表示する

GAL1, GALN, GLNN, GAL, Galanin peptides

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] (AB254556)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] (AB254556)

Immunohistochemical analysis of paraffin-embedded human pituitary gland tissue labeling Galanin with ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human pituitary gland (PMID : 10098517) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254556 for 10 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] (AB254556)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] (AB254556)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Galanin with ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining on human liver (PMID : 2448788) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254556 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] (AB254556)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] (AB254556)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Galanin with ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on myenteric nerves of human colon (PMID : 2417157) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254556 for 10 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] (AB254556)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] (AB254556)

Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue labeling Galanin with ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse pituitary gland (PMID : 10098517) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254556 for 10 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] (AB254556)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] (AB254556)

Immunohistochemical analysis of paraffin-embedded rat pituitary gland tissue labeling Galanin with ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat pituitary gland (PMID : 10098517) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254556 for 10 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] (AB254556)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] (AB254556)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Galanin with ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining on mouse spleen (PMID : 2448788) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254556 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Galanin antibody [EPR22496-308] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR22496-308

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB262924

Anti-Galanin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Galanin antibody (AB262924)

  • 0
  • 0
Primary Antibodies

AB255295

Anti-Galanin antibody [EPR22496-254]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-254] (AB255295)

  • 0
  • 0
Primary Antibodies

AB99452

Anti-Galanin antibody

Western blot - Anti-Galanin antibody (AB99452)

  • 3
  • 1
Primary Antibodies

AB255699

Anti-Galanin antibody [EPR22496-254] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-254] - BSA and Azide free (AB255699)

  • 0
  • 0
Primary Antibodies

AB245676

Anti-Galanin antibody [4B3]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [4B3] (AB245676)

  • 0
  • 0

組合せ製品

Assay Kits

AB270888

Human Galanin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Galanin ELISA Kit (AB270888)

  • 0
  • 0
Primary Antibodies

AB137869

Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

  • 5
  • 9
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 80
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB281583

Anti-CD31 antibody [RM1006]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM1006] (AB281583)

  • 5
  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Galanin also known as the galanin peptide is a neuropeptide that plays a role in various physiological processes. It has a molecular mass of approximately 3 kDa and is expressed widely throughout the central and peripheral nervous systems. Galanin is extensively distributed in the brain spinal cord and gut indicating its involvement in multiple neural functions.
Biological function summary

Galanin influences neurotransmitter release and modulates synaptic transmission. It often forms part of a receptor complex with galanin receptors GALR1 GALR2 and GALR3 which mediate its effects. Galanin exerts diverse actions including regulation of mood feeding behavior and modulation of nociception. These broad biological roles highlight its importance in maintaining homeostasis.

Pathways

Galanin takes part in the regulation of several key signaling pathways such as the MAPK/ERK and the cAMP-PKA pathways. It interacts with other neuropeptides and its activity can modulate the action of neurotransmitters like norepinephrine and serotonin which influence mood and stress response. These pathways reveal its integrative role in neurophysiological regulation and its connection with multiple proteins.

Galanin is linked with conditions such as depression and epilepsy. Changes in galanin expression have been observed in depressive disorders where it may interact with serotonin transporter proteins affecting mood regulation. In epilepsy galanin has a potential role in reducing seizure susceptibility possibly in relation to its interaction with the gamma-aminobutyric acid (GABA) system. Understanding these associations aids in exploring galanin as a potential therapeutic target.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Endocrine hormone of the central and peripheral nervous systems that binds and activates the G protein-coupled receptors GALR1, GALR2, and GALR3. This small neuropeptide may regulate diverse physiologic functions including contraction of smooth muscle of the gastrointestinal and genitourinary tract, growth hormone and insulin release and adrenal secretion.
See full target information GAL
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of veterinary research 68:633-642 PubMed39776677

2025

The effect of prenatal fumonisin B exposure on bone innervation in newborn Wistar rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ewa Tomaszewska,Piotr Dobrowolski,Aleksandra Dajnowska,Liwia Arbatowska,Iwona Puzio,Halyna Rudyk,Oksana Brezvyn,Ihor Kotsyumbas,Janine Donaldson,Jadwiga Śliwa,Marcin B Arciszewski,Siemowit Muszyński

The FEBS journal 289:2877-2894 PubMed34839587

2021

Distinct binding kinetics of E-, P- and L-selectins to CD44.

Applications

Unspecified application

Species

Unspecified reactive species

Linda Li,Qihan Ding,Jin Zhou,Yi Wu,Mingkun Zhang,Xingming Guo,Mian Long,Shouqin Lü
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

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